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Identification of EBV-Specific Antigens Following Microinjection of Subgenomic DNA Fragments

  • Ann Boyd
  • Jay Stoerker
  • Jane Holliday
  • Ronald Glaser
Part of the Developments in Medical Virology book series (DIMV, volume 1)

Abstract

The regions of the Epstein-Barr Virus (EBV) genome which code for proteins within the early antigen (EA) and viral capsid antigen (VCA) complex were identified by indirect immunofluorescence (IF) 2–4 days after microinjection of subgenomic cloned fragments of EBV DNA. Two new regions have been identified as part of the early antigen (EA) complex, namely, the Charon 4A cloned fragments which cover map units 38–47 and 83–93 respectively. One DNA fragment from map units 45–54, produces a protein in human cells after microinjection which reacts with EA-VCA+ human sera. Attempts to transform human B-lymphocytes from cord blood with a variety of EBV DNA fragments is described.

Keywords

Phate Buffer Saline Early Antigen Viral Capsid Antigen 15ml Conical Centrifuge Tube Subgenomic Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Ann Boyd
    • 1
  • Jay Stoerker
    • 2
  • Jane Holliday
    • 2
  • Ronald Glaser
    • 2
  1. 1.Department of BiologyHood College and Program Resources, Inc., NCI-Frederick Cancer Research FacilityFrederickUSA
  2. 2.Department of Medical Microbiology and ImmunologyThe Ohio State University College of Medicine and Comprehensive Cancer CenterColumbusUSA

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