Identification of EBV-Specific Antigens Following Microinjection of Subgenomic DNA Fragments
The regions of the Epstein-Barr Virus (EBV) genome which code for proteins within the early antigen (EA) and viral capsid antigen (VCA) complex were identified by indirect immunofluorescence (IF) 2–4 days after microinjection of subgenomic cloned fragments of EBV DNA. Two new regions have been identified as part of the early antigen (EA) complex, namely, the Charon 4A cloned fragments which cover map units 38–47 and 83–93 respectively. One DNA fragment from map units 45–54, produces a protein in human cells after microinjection which reacts with EA-VCA+ human sera. Attempts to transform human B-lymphocytes from cord blood with a variety of EBV DNA fragments is described.
KeywordsPhate Buffer Saline Early Antigen Viral Capsid Antigen 15ml Conical Centrifuge Tube Subgenomic Fragment
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