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The Study of Transport and Enzymatic Processes in Reconstituted Biological Systems

  • Chapter
Physiology of Membrane Disorders

Abstract

Reconstitution of biological systems has proven a most valuable tool for the elucidation of multicomponent biochemical pathways. The study of a biological activity in both partially resolved or highly purified systems necessitates the assay of a specific, well-defined functional property of an enzyme or protein complex. In contrast to studies of soluble enzymatic complexes in homogeneous solution wherein the reactants and products may be readily distinguished from each other, the biological activity of a number of membrane processes is the translocation of a species from one compartment to another via passive, coupled, or active transport mechanisms. The biological activity may also be dependent upon a receptor-ligand interaction resulting in the change in a membrane permeability or the activation of an enzymatic complex. Each of these different functions, namely passive transport systems, receptor or voltage-dependent permeabilities, coupled transport, and the energy-transducing ATPases, is amenable to studies in reconstituted membranes.

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Authors and Affiliations

  1. Department of Physiology and Cell Biology, University of Texas Health Science Center, Houston, Texas, 77025, USA

    William P. Dubinsky

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Editors and Affiliations

  1. University of Texas Health Science Center at Houston, Houston, Texas, USA

    Thomas E. Andreoli M.D.  & Stanley G. Schultz M.D.  & 

  2. Yale University School of Medicine, New Haven, Connecticut, USA

    Joseph F. Hoffman Ph.D.

  3. University of California, San Diego La Jolla, California, USA

    Darrell D. Fanestil M.D.

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© 1986 Plenum Publishing Corporation

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Dubinsky, W.P. (1986). The Study of Transport and Enzymatic Processes in Reconstituted Biological Systems. In: Andreoli, T.E., Hoffman, J.F., Fanestil, D.D., Schultz, S.G. (eds) Physiology of Membrane Disorders. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2097-5_10

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  • DOI: https://doi.org/10.1007/978-1-4613-2097-5_10

  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4613-2097-5

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