Abstract
Bleomycin is a glycopeptide antibiotic with antineoplastic activity produced by cultures of Streptomyces verticillus. Although it was isolated by Umezawa and his collaboratorsS(1,2) as a copper chelate, it is used clinically as a metal-free preparation. Studies with bleomycin have suggested that its cytotoxic properties are related to effects of the compound on the physical integrity of DNA in cells. In cultures of bacterial or eukaryotic cells, a major effect of bleomycin is the introduction of strand breaks into DNA(3). Breakage of DNA may be demonstrated after incubation of bleomycin with purified DNA. If this in vitro degradation reaction is to proceed with greatest efficiency, it requires the presence of a reducing agent, although a small amount of DNA breakage may be observed in the absence of reducing agent(4,5).
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Horwitz, S.B., Sausville, E.A., Peisach, J. (1979). A Role for Iron in the Degradation of DNA by Bleomycin. In: Hect, S.M. (eds) Bleomycin: Chemical, Biochemical, and Biological Aspects. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-6191-9_13
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DOI: https://doi.org/10.1007/978-1-4612-6191-9_13
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