Abstract
The cyclic nucleotide phosphodiesterases (PDEases) comprise a veiy complex class of enzymes. Numerous forms of the enzyme have been characterized partially using standard biochemical and immunological tools which has led us to a partial understanding of the various forms. The enzyme class has been subgrouped into at least six different families, based on substrate specificity and affinity, molecular mass, and sensitivity to cofactors and drugs (Beavo, 1987). However, the enzymes have been difficult to work with biochemically and the past problems, incomplete nature of the information gathered, and inconsistencies have indicated a need to approach an understanding of the enzymes in a new way. We have adopted the premise that the current insufficiency of information regarding the enzymes can best be filled by isolating the genes and cDNA clones which encode the enzymes. Thus, if every PDEase gene were cloned, we could classify the enzymes according to their gene and/or RNA. Insightful results have already been obtained from the nucleic acids approach.
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References
Beavo, J.A., 1987, Multiple isozymes of cyclic nucleotide phosphodiesterase, in Advances in Second Messenger and Phosphoprotein Research (eds. Robison, G.A. and Greengard, P.) (Raven Press, New York) 22:1–38.
Chen, C.-N., Denome, S., and Davis, R.L., 1986, Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase, Proc. Natl Acad. Sci. USA 83:9313–9317.
Chen, C.-N., et al., 1987, At least two genes reside within a large intron of the dunce gene of Drosophila, Nature (London) 329:721–724.
Colicelli, J., et al., 1989, Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase, Proc. Natl Acad. Sci. USA 86:3599–3603.
Davis, C.W., 1984, Assessment of selective inhibition of rat cerebral cortical calcium-independent and calcium-dependent phosphodiesterases in crude extracts using deoxycylic AMP and potassium ions, Biochim. Biophys. Acta 797:354–362.
Davis, R.L., and Kauvar, L.M., 1984a, Drosophila cyclic nucleotide phosphodiesterases, Adv. Cyclic Nuc. Prot. Phosphor. Res. 16:393–402.
Davis, R.L., and Davidson, N., 1984b, Isolation of the Drosophila melanogaster dunce chromosomal region and recombinational mapping of dunce sequences with restriction site polymorphisms as genetic markers, Mol. Cell. Biol. 4:358–367.
Davis, R.L., and Davidson, N., 1986, The memory gene dunce+ encodes a remarkable set of RNAs with internal heterogeneity, Mol. Cell. Biol. 6:1464–1470.
Davis, R.L., 1988, Mutational analysis of phosphodiesterase in Drosophila, Meth. Enzymol. 159:786–792.
Davis, R.L., et al., 1989, Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene, Proc. Natl Acad. Sci. USA 86:3604–3608.
Dudai, Y., et al., 1976, dunce, a mutant of Drosophila deficient in learning, Proc. Natl. Acad. Sci. USA 73:1684–1688.
Eckmann, F., et al., 1988, Rolipram in major depression: results of a double-blind comparative study with amitriptyline, Curr. Therap. Res. 38:23–39.
Horowski, R., and Sastre-Y-Hernandez, M., 1985, Clinical effects of the neurotropic selective cAMP phosphodiesterase inhibitor rolipram in depressed patients: global evaluation of the preliminary reports, Curr. Therap. Res. 38:23–39.
Pittler, S.J., et al., 1989, Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase α-subunit and chromosomal localization of the human gene, submitted.
Swinnen, J.V., Joseph, D.R., and Conti, M., 1989, Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes, Proc. Natl. Acad. Sci. USA 86:5325–5329.
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Davis, R.L., Henkel-Tigges, J., Qiu, Y., Nighorn, A. (1990). Phosphodiesterase Genes from Flies to Mammals. In: Jacobson, K.A., Daly, J.W., Manganiello, V. (eds) Purines in Cellular Signaling. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3400-5_42
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DOI: https://doi.org/10.1007/978-1-4612-3400-5_42
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