Abstract
A reporter once asked, “isn’t it true that human gene therapy would not have been possible without the development of polymerase chain reaction?” The investigator said no. Afterward, the investigator realized that the reporter was partially correct. The power of PCR technology (Mullis and Faloona, 1987; Saiki et al., 1988) has allowed some remarkable accomplishments, e.g., the detection of one marked cell in a million unmarked cells (Crescenzi et al., 1988), direct gene isolation and sequencing from bulk cellular DNA (Engelke et al., 1988), the analysis of DNA from a single human sperm (Honghua et al., 1988), and, perhaps, aspects of the development of human gene therapy. We must acknowledge that PCR was essential to the first human gene therapy experiments, not conceptually, but because only PCR permitted the unambiguous detection of gene-engineered cells from biological specimens and this technical ability was key to approval of the first gene therapy protocol.
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Morgan, R.A., Anderson, W.F. (1994). Gene Therapy. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_30
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DOI: https://doi.org/10.1007/978-1-4612-0257-8_30
Publisher Name: Birkhäuser, Boston, MA
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