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Birkhäuser

The Polymerase Chain Reaction

  • Book
  • © 1994

Overview

Editors:
  1. Kary B. Mullis

    1. La Jolla, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. François Ferré

    1. The Immune Response Corporation, Carlsbad, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Richard A. Gibbs

    1. Institute for Molecular Genetics, Baylor College of Medicine, Houston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

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Table of contents (35 chapters)

  1. Front Matter

    Pages i-xxii
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  2. Methodology

    1. Front Matter

      Pages 1-1
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    2. Basic Methodology

      1. Manipulation of DNA by PCR
        • Kenshi Hayashi
        Pages 3-13
      2. Cloning PCR Products
        • Michael A. Frohman
        Pages 14-37
      3. Optimization of Multiplex PCRs
        • Jeffrey S. Chamberlain, Joel R. Chamberlain
        Pages 38-46
      4. Preparation of Nucleic Acids for Archival Material
        • Darryl Shibata
        Pages 47-54
      5. PCR Amplification of Viral DNA and Viral Host Cell mRNAs <i>in Situ</i>
        • Janet Embretson, Katherine Staskus, Ernest Retzel, Ashley T. Haase, Peter Bitterman
        Pages 55-64

    3. Quantitation

      1. Quantitative PCR: An Overview
        • F. Ferré, A. Marchese, P. Pezzoli, S. Griffin, E. Buxton, V. Boyer
        Pages 67-88
      2. Quantification of DNAs by the Polymerase Chain Reaction Using an Internal Control
        • Donald M. Coen
        Pages 89-96
      3. RT-PCR and mRNA Quantitation
        • Jamel Chelly, Axel Kahn
        Pages 97-109
      4. Analysis of Human T-Cell Repertoires by PCR
        • Dominic G. Spinella, James M. Robertson
        Pages 110-120

    4. Nonisotopic Detection

      1. Ultrasensitive Nonradioactive Detection of PCR Reactions: An Overview
        • Richard H. Tullis
        Pages 123-133
      2. Fluorescent Detection Methods for PCR Analysis
        • Michael J. Heller
        Pages 134-141
      3. Enzyme-Labeled Oligonucleotides
        • Eugene Tu, Edward Jablonski
        Pages 142-150
      4. Application of the Hybridization Protection Assay (HPA) to PCR
        • Norman C. Nelson, Sherrol H. McDonough
        Pages 151-161

    5. Instrumentation

      1. PCR Instrumentation: Where Do We Stand?
        • Christian C. Oste
        Pages 165-173
      2. Rapid Cycle DNA Amplification
        • Carl T. Wittwer, Gudrun B. Reed, Kirk M. Ririe
        Pages 174-181
      3. Automating the PCR Process
        • H. R. Garner
        Pages 182-198

    6. Sequencing

      1. PCR and DNA Sequencing
        • Bjorn Andersson, Richard A. Gibbs
        Pages 201-213
      2. Phage Promoter-Based Methods for Sequencing and Screening for Mutations
        • Steve S. Sommer, Erica L. Vielhaber
        Pages 214-221
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Keywords

About this book

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc­ ture we had divined from a minimum of experimental data and on theoretical argu­ ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Editors and Affiliations

  • La Jolla, USA

    Kary B. Mullis

  • The Immune Response Corporation, Carlsbad, USA

    François Ferré

  • Institute for Molecular Genetics, Baylor College of Medicine, Houston, USA

    Richard A. Gibbs

Bibliographic Information

  • Book Title: The Polymerase Chain Reaction

  • Editors: Kary B. Mullis, François Ferré, Richard A. Gibbs

  • DOI: https://doi.org/10.1007/978-1-4612-0257-8

  • Publisher: Birkhäuser Boston, MA

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Boston 1994

  • Hardcover ISBN: 978-0-8176-3607-4Published: 01 April 1994

  • Softcover ISBN: 978-0-8176-3750-7Published: 01 March 1994

  • eBook ISBN: 978-1-4612-0257-8Published: 02 February 2012

  • Edition Number: 1

  • Number of Pages: XXII, 458

  • Number of Illustrations: 85 b/w illustrations

  • Topics: Pharmacy

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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