Advertisement

Liver Cancer pp 343-350 | Cite as

Hepatitis C Virus-Related Human Hepatocellular Carcinoma: Predictive Markers Using Proteomic Analysis (Methodology)

  • Yasuhiro Kuramitsu
  • Kazuyuki Nakamura
Chapter
  • 1.7k Downloads
Part of the Methods of Cancer Diagnosis, Therapy and Prognosis book series (HAYAT, volume 5)

It was reported by El-Serag and Mason (1999) that hepatocellular carcinoma (HCC) is one of the most common fatal cancers world-wide. From the repots by Okuda (1992, 2000) and Seow et al. (2001), the most apparent risk factor for HCC is thought to be the chronic infection with hepatitis B or C virus (HBV or HCV). Although in the other Asian countries HCCs developed from HBV-infection, the characteristic of HCCs in Japan is that they develop from the liver tissues infected with HCV or of liver cirrhosis. From the reports by Yano et al. (2003) and Yatsuhashi (2004), 80% of the death cases of HCC patients have HCV-infection, and the number of HCV-carriers in Japan is 2 million. The number of patients with HCC is still on the increase, although diagnostic and therapeutic techniques have become remarkably advanced. It was reported by Yatsuhashi (2004) that the incidence of HCC is increasing in many developed countries in parallel to an increase in chronic HCV infection. Therefore, it is important to identify the predictive marker proteins by which we can predict the carcinogenesis of HCC in HCV-infected patients.

Keywords

Glutamine Synthetase Protein Spot Ammonium Bicarbonate Noncancerous Tissue Triosephosphate Isomerase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. El-Serag, H. B., and Mason, A. C. 1999. Rising incidence of hepatocellular carcinoma in the United States. N. Eng. J. Med. 340: 745–750.CrossRefGoogle Scholar
  2. Ha, G.H., Lee, S.U., Kang, D.G., Ha, N.Y., Kim, S.H., Kim, J., Bae, J.M., Kim, J.W., and Lee, C.W. 2002. Proteome analysis of human stomach tissue: separation of soluble proteins by two-dimensional polyacrylamide gel electro-phoresis and identification by mass spectrom-etry. Electrophoresis 23: 2513–2524.PubMedCrossRefGoogle Scholar
  3. Kim, W., Lim, S.O., Kim, J-S., Ryu, Y.H., Byeon, J.Y., Kim, H.J., Kim, Y.I., Heo, J.S., Park, Y.M., and Jung, G. 2003. Comparison of pro-teome between hepatitis B virus- and hepatitis C virus-associated hepatocellular carcinoma. Clin. Cancer Res. 9: 5493–5500.PubMedGoogle Scholar
  4. Kuramitsu, Y., Harada, T., Takashima, M., Yokoyama, Y., Hidaka, I., Iizuka, N., Toda, T., Fujimoto, M., Zhang, X., Sakaida, I., Okita, K., Oka, M. and Nakamura, K. 2006. Increased expression, and phosphorylation of liver glutamine synthetase in well-differentiated hepatocellular carcinoma tissues of patients infected with hepatitis C virus. Electrophoresis 27: 1651–1658.PubMedCrossRefGoogle Scholar
  5. Li, C., Tan, Y-X., Zhou, H. Ding, S.J., Li, S.J., Ma, D.J., Man, X.B., Hong, Y., Zhang, L., Li, L., Xia, Q.C., Wu, J.R,, Wang, H.Y., and Zeng, R. 2005. Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers. Proteomics 5: 1125–1139.PubMedCrossRefGoogle Scholar
  6. Okuda, K. 1992. Hepatocellular carcinoma: recent progress. Hepatology 15: 948–963.PubMedCrossRefGoogle Scholar
  7. Okuda, K. 2000. Hepatocellular carcinoma. J. Hepatol. 32: 225–237.PubMedCrossRefGoogle Scholar
  8. Paradis, V., Degos, F., Dargere, D., Pham, N., Belghiti, J., Degott, C., Janeau, J.L., Bezeaud, A., Delforge, D., Cubizolles, M., Laurendeau, I., and Bedossa, P. 2005. Identification of a new marker of hepatocellular carcinoma by serum profiling of patients with chronic liver diseases. Hepatology 41: 40–47.PubMedCrossRefGoogle Scholar
  9. Seow, T. K., Liang, R. C. Y., Leow, C. K., and Chung, M. C. M., 2001. Hepatocellular carcinoma: from bedside to proteomics. Proteomics 1: 1249–1263.PubMedCrossRefGoogle Scholar
  10. Simpson, R.J., Connolly, L.M., Eddes, J.S., Pereira, J.J., Moritz, R.L., and Reid, G.E. 2000. Proteomic analysis of the human colon carcinoma cell line (LIM 1215): development of a membrane protein database. Electrophoresis 21: 1707–1732.PubMedCrossRefGoogle Scholar
  11. Steel, L.F., Mattu, T.S., Mehta, A., Hebestreit, H., Dwek, R., Evans, A. A., London, W.T., and Block, T. 2001. A proteomic approach for the discovery of early detection markers of hepato-cellular carcinoma. Dis. Markers 17: 179–189.PubMedGoogle Scholar
  12. Steel, L.F., Shumpert, D., Trotter, M., Seeholzer, S.H., Evans, A.A., London, W.T., Dwek, R., and Block, T.M. 2003. A strategy for the comparative analysis of serum proteomes for the discovery of biomarkers for hapatocellular carcinoma. Proteomics 3: 601–609.PubMedCrossRefGoogle Scholar
  13. Takashima, T., Kuramitsu, Y., Yokoyama, Y., Iizuka, N., Toda, T., Saiakida, I., Okita, K., Oka, M., and Nakamura, K. 2003. Proteomic profiling of heat shock protein 70 family members as biomarkers for hepatitis C virus-related hepatocellular carcinoma. Proteomics 3: 2487–2493.PubMedCrossRefGoogle Scholar
  14. Takashima, T., Kuramitsu, Y., Yokoyama, Y., Iizuka, N., Fujimoto, M., Nishisaka, T., Sakaida, I., Okita, K., Oka, M., and Nakamura, K. 2005. Overexpression of alpha enolase in hepatitis C virus-related hepatocellular carcinoma: association with tumor progression as determined by proteomic analysis. Proteomics 5: 1686–1692.PubMedCrossRefGoogle Scholar
  15. Takashima, T., Kuramitsu, Y., Yokoyama, Y., Iizuka, N., Fujimoto, M., Sakaida, I., Okita, K., Oka, M., and Nakamura, K. 2006. Proteomic analysis of autoantibodies in patients with hepa-tocellular carcinoma. Proteomics 6: 3894–3900.PubMedCrossRefGoogle Scholar
  16. Wasinger, V.C., Cordwell, S.J., Cerpa-Poljak, A., Yan, J.X., Gooley, A.A., Wilkins, M.R., Duncan, M.W., Harris, R., Williams, K.L., and Humphery-Smith, I. 1995. Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis 16: 1090–1094.PubMedCrossRefGoogle Scholar
  17. Yano, K., Yatsuhashi, H., and Yano, M., 2003. Hepatitis C. Nippon Rinsho 61: 241–244.PubMedGoogle Scholar
  18. Yatsuhashi, H., 2004. General remarks on chronic hepatitis C. Nippon Rinsho 62: 400–404.PubMedGoogle Scholar
  19. Yokoyama, Y., Kuramitsu, Y., Takashima, T., Iizuka, N., Toda, T., Terai, S., Sakaida, I., Oka, M., Nakamura, K., and Okita, K. 2004. Proteomic profiling of proteins decreased in hepatocellular carcinoma from patients infected with hepatitis C virus. Proteomics 4: 2111–2116.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V. 2009

Authors and Affiliations

  • Yasuhiro Kuramitsu
    • 1
  • Kazuyuki Nakamura
    • 1
  1. 1.Department of Biochemistry and Functional ProteomicsYamaguchi University Graduate School of MedicineMinami-Kogushi, UbeJapan

Personalised recommendations