Cryopreservation of Stem Cells

Institutional achievements in research of low temperature preservation of stem cells derived from fetal and adult sources are presented in the report. Special attention is attended to cryopreservation of pretenders on hemopoietic stem cells from human cord blood and fetal liver. Examining of viability of cryopreserved with DMSO fetal liver cells of specific phenotype by parallel determining with vital dye has demonstrated that CD133+ and CD34+cell candidates occurred to be more sensitive to programmed cryopreservation in comparison with more differentiated erythroid precursors (glycophorin-A – positive cells). No differences in viabilities between CD 45-, CD 133- and CD 34- positive cells after cryopreservation of primary suspension of fetal liver cells was revealed. Cryopreservation of cord blood nucleated cells with PEO-1500 allowed to obtain higher viability of hemopoietic stem CD 34+-cells in respect of CD45+-cells. Presented data demonstrated that hemopoietic cells of human fetal liver and cord blood of various phenotypes were characterized with different sensitivity to cryopreservation. Candidates to stem hemopoietic cells, obtained from two different sources: human fetal liver and cord blood, demonstrate quite a high viability after cryopreservation with various methods using cryoprotectants with different mechanisms of action.

Keywords: HSC; cryopreservation; viability