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Progress Towards the Anatomy of the Eukaryotic DNA Replication Fork

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Genome Integrity

Part of the book series: Genome Dynamics and Stability ((GENOME,volume 1))

Abstract

During cell growth before each division, cells have to accurately duplicate their genome. The processes associated with DNA replication are tightly controlled; failure thereof can result in genome instability, which is a hallmark of cancer (Marte 2004). A large number of proteins are involved in this ambitious task to replicate the chromosomal DNA once and only once at a given time (Table 1).

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Abbreviations

AAA:

ATPases associated with a variety of cellular activities

ATR:

Ataxia telangiectasia-mutated and Rad3-related

ATRIP:

ATR interacting protein

BRCA:

breast cancer associated

BRCT:

BRCA1 C-terminal

CAF:

chromatin assembly factor

Cdc:

cell division cycle

Cdt:

Cdc10-dependent

Cdk:

cyclin-dependent kinase

CKI:

Cdk inhibitor

Dbf:

dumbbell former

DBD:

DNA binding domain

DDK:

Dbf4-dependent kinase

DPB :

DNA polymerase epsilon subunit B

Drf:

Dbf4-related factor

dsDNA:

double-stranded DNA

FEN:

flap endonuclease

GINS:

Go Ichi, Nii, and San Japanese for five, one, two, and three; protein complex containing Sld5 and Psf1-3

Mcm:

minichromosome maintenance

Mus:

nitrogen mustard-sensitive

OB:

oligonucleotide/oligosaccharide binding

ORC:

origin recognition complex

PARP:

poly[ADP-ribose]polymerase

PCNA:

Proliferating cell nuclear antigen

pre-RC:

pre-replicative complex

Pol:

DNA polymerase

POL2 :

DNA polymerase epsilon catalytic subunit in S. cerevisiae

Psf:

partner of Sld five

Rad:

radiation-sensitive

rec:

recombination defective

RF-C:

replication factor C

RPA:

replication protein A [eukaryotic ssDNA binding protein]

Srs:

suppressor of radiation-sensitive mutations

ssDNA:

single-stranded DNA

Sld:

synthetically lethal with D PB11

SUMO:

small ubiquitin-related modifier protein

SV:

simian virus

TAg:

T antigen

TopBP1:

DNA topoisomerase II binding protein

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  1. National University of Ireland, Galway, Dept. of Biochemistry, Cell Cycle Control Laboratory, University Road, Galway, Ireland

    Heinz Peter Nasheuer

  2. University of Oulu, Department of Biochemistry, P.O. Box 3000, 90014, Oulu, Finland

    Helmut Pospiech

  3. University of Joensuu, Department of Biology, P.O. Box 111, 80101, Joensuu, Finland

    Juhani Syväoja

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Nasheuer, H.P., Pospiech, H., Syväoja, J. (2006). Progress Towards the Anatomy of the Eukaryotic DNA Replication Fork. In: Lankenau, DH. (eds) Genome Integrity. Genome Dynamics and Stability, vol 1. Springer, Berlin, Heidelberg . https://doi.org/10.1007/7050_016

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