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Protein Arrays and Fluorescence Detection: Applications and Limitations

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Standardization and Quality Assurance in Fluorescence Measurements II

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 6))

Abstract

Within genomics, spotted microarrays have become an increasingly important research tool for the biotechnology industry, molecular diagnostics, drug screening, nucleic acid sequencing, and mutation analysis. Working with proteins on arrays is especially difficult, because the native state of proteins is extremely sensitive to pH, ionic strength, temperature, and protein concentration. Since optimized conditions differ from protein to protein, the development of in vitro protein-based assays always presents a big challenge. In this chapter, the design and applications of peptide and protein arrays will be addressed. Different labeling chemistries for the analytes and signal detection procedures, with special emphasis dedicated to quality assurance related issues, are discussed. Possible applications of protein arrays, such as epitope mapping of autoantibodies, binding assays, analysis of protein expression, and protein modification, as well as monitoring of enzymatic activity, shed light on the high versatility of protein arrays. However, much more research and development will be necessary, until they meet the requirements of laboratories involved in high-throughput screenings and diagnostics.

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Authors and Affiliations

  1. University of Applied Sciences, Department of Biotechnology, Paul Wittsack Strasse 10, 68163, Mannheim, Germany

    Christian Maercker

  2. German Cancer Research Center, Genomics and Proteomics Core Facilities, Im Neuenheimer Feld 515, 69120, Heidelberg, Germany

    Christian Maercker

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  1. Christian Maercker
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Correspondence to Christian Maercker .

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Ute Resch-Genger

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© 2008 Springer-Verlag Berlin Heidelberg

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Maercker, C. (2008). Protein Arrays and Fluorescence Detection: Applications and Limitations. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements II. Springer Series on Fluorescence, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_040

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