Abstract
The ability of fluorescence to obtain temporal information on chemical and physical processes in molecules and supramolecular systems is of major importance. A time resolution of a few tens of picoseconds can nowadays be easily achieved by pulse and phase fluorometries, and the time resolution can be extended to femtosecond phenomena by more recent techniques based on fluorescence up-conversion.
In this chapter the most common methods used in time-resolved fluorometry will be discussed. The purpose is to introduce the techniques and highlight their application range, typical problems to be solved and the limitations imposed by each method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Macklin JJ, Trautman JK, Harris TD, Brus LE (1996) Science 272:255
Vallee RAL, Marsal P, Braeken E, Habuchi S, De Schryver FC, Van der Auweraer M, Beljonne D, Hofkens J (2005) J Am Chem Soc 127:12011
Zewail AH (2000) J Phys Chem A 104:5660
Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer/Plenum, New York
Valeur B (2002) Molecular fluorescence: principles and applications. Wiley-VCH, Weinheim
Tkachenko NV (2006) Optical spectroscopy: methods and instrumentations. Elsevier, Amsterdam
Maus M, Cotlet M, Hofkens J, Gensch T, De Schryver FC, Schaffer J, Seidel CAM (2001) Anal Chem 73:2078
Sash J (1988) IEEE J Quant Electron 24:276
Kesti TJ, Tkachenko NV, Vehmanen V, Yamada H, Imahori H, Fukuzumi S, Lemmetyinen H (2002) J Am Chem Soc 124:8067
Zhao L, Lustres JLP, Farztdinov V, Ernsting NP (2005) Phys Chem Chem Phys 7:1716
Kinoshita S, Ozawa H, Kanematsu Y, Tanaka I, Sugimoto N, Fujiwara S (2000) Rev Sci Instrum 71:3317
Schmidt B, Laimgruber S, Zinth W, Gilch P (2003) Appl Phys B 76:809
Booth MJ, Wilson T (2004) J Microsc 214:36
Philip J, Carlsson K (2003) J Opt Soc Am A 20:368
Gulbinas V, Chachisvilis M, Valkunas L, Sundström V (1996) J Phys Chem 100:2219
Kalinin S, Johansson LB-Å (2004) J Phys Chem B 108:3092
Dhaka VDS, Tkachenko NV, Lemmetyinen H, Pavelescu E-M, Suomalainen S, Pessa M, Arsila K, Nordlund K, Keinonen J (2006) J Phys D Appl Phys 39:2659
Author information
Authors and Affiliations
Corresponding author
Editor information
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Tkachenko, N.V., Lemmetyinen, H. (2008). Time-Resolved Fluorometry: Typical Methods, Challenges, Applications and Standards. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements I. Springer Series on Fluorescence, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_031
Download citation
DOI: https://doi.org/10.1007/4243_2008_031
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-75206-6
Online ISBN: 978-3-540-75207-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)