Abstract
The precision of analytical methods using fluorescent probes or biomolecular labels often is compromised of a variety of conceivable interferences that may originate from the instrumental system, the sample, or the underlying sensor chemistry. Instrumental drifts of the optoelectronic system, photobleaching of luminophores, or high intrinsic color and background fluorescence of the sample cannot be eliminated even by extensive calibration procedures. As a result, intrinsically referenced methods are preferred to improve optical chemo- or biosensor technology, and fluorescent bioimaging. Intrinsic referencing is often accomplished by ratiometric techniques. These include (1) dual wavelength probes, (2) dual luminophore sensors, (3) lifetime-based assays, (4) dual lifetime referencing (in the time and frequency-domain), and (5) fluorescence anisotropy. Applications and advantages of the various approaches are outlined in this review, with a focus on widely used sensing methods for oxygen, pH, carbon dioxide, calcium, glucose, or temperature, and on biomolecular screening. In addition to ratiometric methods, fluorescence correlation spectroscopy represents another attractive tool to determine analyte concentrations via fluorescent probes. Many of these ratiometric approaches have the potential to pave the way for the development of calibration-free sensor and imaging schemes.
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References
Wolfbeis OS, Böhmer M, Dürkop A, Enderlein J, Gruber M, Klimant I, Krause C, Kürner J, Liebsch G, Lin Z, Oswald B, Wu M (2002) Advanced Luminescent Labels, Probes and Beads, and Their Application to Luminescence Bioassay and Imaging. In: Kraayenhof R, Visser AJWG, Gerritsen HC (eds) Springer Series on Fluorescence 2 (Fluorescence Spectroscopy, Imaging and Probes). Springer, Berlin Heidelberg New York, pp 3–42
Schäferling M (2005) Luminescence Lifetime-Based Imaging of Sensor Arrays for High-Throughput Screening Applications. In: Orellana G, Moreno-Bondi MC (eds) Springer Series on Chemical Sensors and Biosensors 3 (Frontiers in Chemical Sensors). Springer, Berlin Heidelberg New York, pp 45–92
Orellana G, Moreno-Bondi MC, Garcia-Fresnadillo D, Marazuela MD (2005) The Interplay of Indicator, Support and Analyte in Optical Sensor Layers. In: Orellana G, Moreno-Bondi MC (eds) Springer Series on Chemical Sensors and Biosensors 3 (Frontiers in Chemical Sensors). Springer, Berlin Heidelberg New York, pp 189–225
Mingoarranz FJ, Moreno-Bondi MC, Garcia-Fresnadillo D, de Dios C, Orellana G (1995) Mikrochim Acta 121:107
Amao Y (2003) Microchim Acta 143:1
Lin J (2000) Trends Anal Chem 19:541
Haugland RP (2002) Handbook of Fluorescent Probes and Research Products. Molecular Probes. http://probes.invitrogen.com/handbook. Accessed Oktober 2007
Amersham Biosciences (2007) Reactive cyanine dyes. http://www.amershambiosciences.com. Accessed Oktober 2007
Horobin RW (1980) J Microsc 119:345
Nagl S, Schäferling M, Wolfbeis OS (2005) Microchim Acta 151:1
Chan WCW, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S (2002) Curr Opin Biotechnol 13:40
Costa-Fernandez JM (2006) Anal Bioanal Chem 384:37
Swanson JA (2002) Ratiometric fluorescence microscopy. In: Sansonetti P, Zychlinsky A (eds) Methods in Microbiology, 31 (Molecular Cellular Microbiology). Elsevier, Amsterdam, pp 1–18
Kostov Y, Rao G (1999) Rev Sci Instrum 70:4466
Wang E, Chow KF, Kwan V, Chin T, Wong C, Bocarsly A (2003) Anal Chim Acta 495:45
Shahriari MR, Ding JY (1994) In: Klein LC (ed) sol–gel Optics: Processing and Applications, chap 13. Kluwer, Boston, p 279
Lobnik A, Oehme I, Murkovic I, Wolfbeis OS (1998) Anal Chim Acta 367:159
Draxler S, Lippitsch ME (1995) Sens Actuators B 29:199
Wroblewski W, Roznicka E, Dybko A, Brzozka Z (1998) Sens Actuators B 48:471
Schulman SG, Chen S, Bai F, Leiner MJP, Weis L, Wolfbeis OS (1995) Anal Chim Acta 304:165
Precision Sensing (2007) http://www.presens.de. Accessed Oktober 2007
Hulth S, Aller RC, Engstrom P, Selander E (2002) Limnol Oceanogr 47:212
Paradiso AM, Tsien RY, Machen TE (1984) Proc Natl Acad Sci USA 81:7436
Muller-Borer BJ, Yang H, Marzouk SAM, Lemasters JJ, Cascio WE (1998) Am J Physiol 275:H1937
Ishaque A, Al-Rubeai M (1998) J Immunol Methods 221:43
Boyer MJ, Hedley DW (1994) Methods Cell Biol 41:135
Qian T, Nieminen AL, Herman B, Lemasters JJ (1997) Am J Physiol 273:C1783
Xu Z, Rollins A, Alcala R, Marchant RE (1998) J Biomed Mater Res 39:9
Parker JW, Laksin O, Yu C, Lau ML, Klima S, Fisher R, Scott I, Atwater BW (1993) Anal Chem 65:2329
Schroeder C, Weidgans BM, Klimant I (2005) Analyst 130:907
Poenie M, Alderton J, Tsien RY, Steinhardt RA (1985) Nature 315:147
Silver RB (1998) Methods Cell Biol 56:237
Xu T, Yang W, Huo XL, Song T (2004) J Biochem Biophys Methods 58:219
Valet G, Raffael A, Rüssmann L (1985) Naturwissenschaften 72:600
Sanchez-Martin RM, Cuttle M, Mittoo S, Bradley M (2006) Angew Chem 118:5598
Lee SK, Lee JY, Lee MY, Chung SM, Chung JH (1999) Anal Biochem 273:186
Sun XP, Callamaras N, Marchant JS, Parker I (1998) J Physiol 509:67
Minta A, Kao JP, Tsien RY (1989) J Biol Chem 264:8171
Oheim M, Naraghi M, Muller TH, Neher E (1998) Cell Calcium 24:71
Lakowicz JR, Szmacinski H, Johnson ML (1992) J Fluoresc 2:47
Budinger GR, Duranteau J, Chandel NS, Schumacker PT (1998) J Biol Chem 273:3320
Meuwis K, Boens N, De Schryver FC, Gallay J, Vincent M (1995) Biophys J 68:2469
Minta A, Tsien RY (1989) J Biol Chem 264:19449
Henary MM, Wu Y, Fahrni CJ (2004) Chem Eur J 10:3015
Peng X, Wu Y, Fan J, Tian M, Han K (2005) J Org Chem 70:10524
Stromberg N, Hulth S (2003) Sens Actuators B 90:308
Jang YJ, Jun EJ, Lee YJ, Kim YS, Kim JS, Yoon J (2005) J Org Chem 70:9603
Deng G, James TD, Shinkai S (1994) J Am Chem Soc 116:4567
Badugu R, Lakowicz JR, Geddes CD (2005) Bioorg Med Chem 13:113
Kaur G, Fang H, Gao X, Li H, Wang B (2006) Tetrahedron 62:2583
Zhao J, James TD (2005) J Mater Chem 15:2896
Yoon J, Czarnik AW (1992) J Am Chem Soc 114:5874
Zhang Y, Gao X, Hardcastle K, Wang B (2006) Chem Eur J 12:1377
Gao X, Zhang Y, Wang B (2005) New J Chem 29:1
Badugu R, Lakowicz JR, Geddes CD (2005) Talanta 66:569
Badugu R, Lakowicz JR, Geddes CD (2004) Anal Biochem 327:82
DiCesare N, Lakowicz JR (2001) J Photochem Photobiol 143:39
Wolfbeis OS, Klimant I, Werner T, Huber C, Kosch U, Krause C, Neurauter G, Duerkop A (1998) Sens Actuators B 51:17
Kaur G, Lin N, Fang H, Wang B (2006) Boronic Acid-Based Fluorescence Sensors for Glucose Monitoring. In: Geddes CD, Lakowicz JR (eds) Topics in Fluorescence Spectroscopy, vol 11 Glucose Sensing. Springer, Berlin Heidelberg New York, pp 377–397
Mingoarranz FJ, Moreno-Bondi MC, Garcia-Fresnadillo D, de Dios C, Orellana G (1995) Microchim Acta 121:107
Amao Y (2003) Microchim Acta 143:1
Molls M, Vaupel P (1998) The Impact of the Tumor Environment on Experimental and Clinical Radiation Oncology and other Therapeutic Modalities. In: Molls M, Vaupel P (eds) Blood Perfusion and Microenvironment of Human Tumors – Implications for Clinical Radiooncology. Springer, Berlin Heidelberg New York
John GT, Klimant I, Wittman C, Heinzle E (2003) Biotechnol Bioeng 81:829
Precision Sensing (2007) Presens OxoPlate. http://www.presens.de. Accessed Oktober 2007
BD Biosciences (2007) BD Oxygen Biosensor System. http://www.bd.com. Accessed Oktober 2007
Deshpande RR, Koch-Kirsch Y, Maas R, John GT, Krause C, Heinzle E (2005) Assay Drug Develop Technol 3:299
Wodnicka M, Guarino RD, Hemperly JJ, Timmins RM, Stitt D, Pitner JB (2000) J Biomol Screen 5:141
Arain S, John GT, Krause C, Gerlach J, Wolfbeis OS, Klimant I (2006) Sens Actuators B 113:639
O'Mahony FC, Papkovsky DB (2006) Appl Environ Microbiol 72:1279
Brasuel M, Kopelman R, Aylott JW, Clark H, Xu H, Hoyer M, Miller TJ, Tjalkens R, Philbert MA (2002) Sens Mater 14:309
Cao Y, Koo Y-EL, Kopelman R (2004) Analyst 129:745
Koo Y-EL, Cao Y, Kopelman R, Koo SM, Sang M, Brasuel M, Philbert MA (2004) Anal Chem 76:2498
Cao Y, Koo Y-EL, Koo SM, Kopelman R (2005) Photochem Photobiol 81:1489
Sumner JP, Westerberg NM, Stoddard AK, Fierke CA, Kopelman R (2006) Sens Actuators B 113:760
McEvoy AK, McDonagh CM, MacCraith BD (1996) Analyst 121:785
Klimant I, Ruckruh F, Liebsch G, Stangelmayer A, Wolfbeis OS (1999) Microchim Acta 131:35
Park EJ, Reid KR, Tang W, Kennedy RT, Kopelman R (2005) J Mater Chem 15:2913
Song A, Parus S, Kopelman R (1997) Anal Chem 69:863
Wolfbeis OS, Weidgans BM (2006) Fiber Optic Chemical Sensors and Biosensors: A View Back. In: Baldini F, Chester AN, Homola J, Martellucci S (eds) Optical Chemical Sensors. Springer, Berlin Heidelberg New York, pp 17–44
Bell JB, Schairer ET, Hand LA, Mehta RD (2001) Annu Rev Fluid Mech 33:155
Engler RH, Merienne MC, Klein C, LeSant Y (2002) Aerospace Sci Technol 6:313
Kingsley-Rowe JR, Lock GD, Davies AG (2003) Royal Aeronaut J 107:1
Asai K, Amao Y, Iijima Y (2002) J Thermophys Heat Transf 16:109
Klein C, Engler RH, Henne U, Sachs WE (2005) Exp Fluids 39:475
Khalil GE, Costin C, Crafton J, Jones G, Grenoble S, Gouterman M, Callis JB, Dalton LR (2004) Sens Actuators B 97:13
Oheim M, Naraghi M, Muller TH, Neher E (1998) Cell Calcium 24:71
Ren Y, Ridsdale A, Coderre E, Stys PK (2000) J Neurosci Methods 102:165
Selvin PR (2000) Nat Struct Biol 7:730
Jares-Erijman EA, Jovin TM (2003) Nat Biotech 21:1387
Kircher MF, Weissleder R, Josephson L (2004) Bioconj Chem 15:242
Takakusa H, Kikuchi K, Urano Y, Kojima H, Nagano T (2003) Chem Eur J 9:1479
Gonzalez-Gomez F, Vergara F, Fernandez A, Pedrosa C, Ramirez JP, Castilla JA, Ruiz R, Galan JJ, Ruiz A, Real LM (2003) Clin Chem Lab Med 41:392
Selvin PR (2002) Ann Rev Biophys Biomol Struct 31:275
Bustin SA (2002) J Mol Endocrinol 29:23
Mere L, Bennett T, Coassin P, England P, Hamman B, Rink T, Zimmerman S, Negulescu P (1999) Drug Discovery Today 4:363
Boute N, Jockers R, Issad T (2002) Trends Pharmacol Sci 23:351
Augustin CM, Oswald B, Wolfbeis OS (2002) Anal Biochem 305:166
Deniz AA, Laurence TA, Dahan M, Chamla DS, Schultz PG, Weiss S (2001) Ann Rev Phys Chem 53:233
Suzuki Y, Yasunaga T, Ohkura R, Wakabayashi T, Sutoh K (1998) Nature 396:380
Hudgins RR, Huang F, Gramlich G, Nau WM (2002) J Am Chem Soc 124:556
Sharma A, Wolfbeis OS (1988) Anal Chim Acta 212:261
Miura M, Miyahara T, Kato M, Toyo'oka T (2004) Anal Chim Acta 501:45
Cano-Raya C, Fernandez-Ramos MD, Capitan-Vallvey LF (2006) Anal Chim Acta 555:299
Valeur B (2002) Molecular Fluorescence. Wiley-VCH, Weinheim
Schneider PC, Clegg RM (1997) Rev Sci Instrum 68:4107
Herman P, Lin HJ, Lakowicz JR (2003) Lifetime-Based Imaging. In: Vo-Dinh T (ed) Biomedical Photonics. CRC, Boca Raton, pp 9.1–9.30
Wang XF, Uchida T, Coleman DM, Minami S (1991) Appl Spectrosc 45:360
Wang XF, Uchida T, Minami S (1989) Appl Spectrosc 43:840
Hartmann P, Ziegler W, Holst G, Lübbers DW (1997) Sens Actuators B 38:110
Woods RJ, Scypinski S, Cline Love LJ, Ashworth HA (1984) Anal Chem 56:1395
Ballew RM, Demas JN (1989) Anal Chem 61:30
Liebsch G, Klimant I, Frank B, Holst G, Wolfbeis OS (2000) Appl Spectrosc 54:548
Hartmann P, Ziegler W (1996) Anal Chem 68:4512
Babilas P, Schacht V, Liebsch G, Wolfbeis OS, Landthaler M, Szeimies R-M, Abels C (2003) Br J Cancer 88:1462
Kellner K, Liebsch G, Klimant I, Wolfbeis OS, Blunk T, Schulz MB, Göpferich A (2002) Biotech Bioeng 80:73
Zhong W, Urayama P, Mycek M-A (2003) J Phys D Appl Phys 36:1689
Hanson KM, Behne MJ, Barry NP, Mauro TM, Gratton E, Clegg RM (2002) Biophys J 83:1682
Sanders R, Draaijer A, Gerritsen HC, Houpt PM, Levine YK (1995) Anal Biochem 227:302
Vroom JM, De Grauw KJ, Gerritsen HC, Bradshaw DJ, Marsh PD, Watson GK (1999) Appl Environ Microbiol 65:3502
Schäferling M, Wu M, Enderlein J, Bauer H, Wolfbeis OS (2003) Appl Spectrosc 57:1386
Lin Z, Wu M, Schäferling M, Wolfbeis OS (2004) Angew Chem Int Ed 43:1735
Duerkop A, Turel M, Lobnik A, Wolfbeis OS (2006) Anal Chim Acta 555:292
Zelelow B, Khalil GE, Phelan G, Carlson B, Gouterman M, Callis JB, Dalton LR (2003) Sens Actuators B 96:304
Schäferling M, Wu M, Wolfbeis OS (2004) J Fluoresc 14:561
Duerkop A, Schäferling M, Wolbeis OS (2006) In: Geddes CD, Lakowicz JR (eds) Topics in Fluorescence Spectroscopy, vol 11 Glucose Sensing. Springer, Berlin Heidelberg New York, pp 351–375
Wu M, Lin Z, Schäferling M, Dürkop A, Wolfbeis OS (2005) Anal Biochem 340:66
Wu M, Lin Z, Wolfbeis OS (2003) Anal Biochem 320:129–135
Lin Z, Wu M, Wolfbeis OS, Schäferling M (2006) Chem Eur J 12:2730
Arakawa T, Akamine M (2003) Sens Acuators B 91:252
Kessler MA (1998) Anal Chim Acta 364:125
Cano-Raya C, Fernandez Ramos MD, Capitan Vallvey LF, Wolfbeis OS, Schäferling M (2005) Appl Spectrosc 59:1209
Liebsch G, Klimant I, Krause C, Wolfbeis OS (2001) Anal Chem 73:4354
Mayr T, Igel C, Liebsch G, Klimant I, Wolfbeis OS (2003) Anal Chem 75:4389
Bambot SB, Sipior J, Lakowicz JR, Rao G (1994) Sens Actuators B 22:181
Von Bültzingslöwen C, McEvoy AK, McDonagh C, MacCraith BD (2003) Anal Chim Acta 480:275
Huber C, Werner T, Krause C, Klimant I, Wolfbeis OS (1998) Anal Chim Acta 364:143
Chan Q, Sipior J, Lakowicz JR, Rao G (1995) Anal Biochem 232:92
Pfeifer L, Stein K, Fink U, Welker A, Wetzl B, Bastian P, Wolfbeis OS (2005) J Fluoresc 15:423
Hoefelschweiger BK, Pfeifer L, Wolfbeis OS (2005) J Biomol Screen 10:687
Hartmann P, Ziegler W, Holst G, Lübbers DW (1997) Sens Actuators B 38/39:110
Szmacinski H, Lakowicz JR (1999) Appl Spectrosc 531:490
Herman P, Maliwal BP, Lakowicz JR (2002) Anal Biochem 309:19
Tolosa L, Gryczynski I, Eichhorn LR, Dattelbaum JD, Castellano FN, Rao G, Lakowicz JR (1999) Anal Biochem 267:114
Chang Q, Randers-Eichhorn L, Lakowicz JR, Rao G (1998) Biotechnol Prog 14:326
Klimant I, Huber C, Liebsch G, Neurauter G, Stangelmayer A, Wolfbeis OS (2002) In: Valeur B and Brochon JC (eds) New Trends in Fluorescence Spectroscopy, Applications to Chemical and Life Sciences (Springer Series on Fluorescence). Springer, Berlin Heidelberg New York, pp 257–274
Klimant I (1997) German Patent Application DE 198 29 657
Lakowicz JR, Castellano JD, Dattelbaum L, Tolosa L, Rao G, Gryczynski I (1998) Anal Chem 70:5115
Kosch U, Klimant I, Werner T, Wolfbeis OS (1998) Anal Chem 70:3892
Dürkop A, Lehmann F, Wolfbeis OS (2002) Anal Bioanal Chem 372:688
Wolfbeis OS, Klimant I, Werner T, Huber C, Kosch U, Krause C, Neurauter G, Dürkop A (1998) Sens Actuators B 51:17
Huber C, Klimant I, Krause C, Werner T, Mayr T, Wolfbeis OS (2000) Fres J Anal Chem 369:196
Huber C, Klimant I, Krause C, Wolfbeis OS (2001) Anal Chem 73:2097
Huber C, Klimant I, Krause C, Werner T, Wolfbeis OS (2001) Anal Chim Acta 449:81
Von Bültzingslöwen C, McEvoy AK, McDonagh C, MacCraith BD, Klimant I, Krause C, Wolfbeis OS (2002) Analyst 127:1478
Mohr GJ, Klimant I, Spichiger-Keller U, Wolfbeis OS (2001) Anal Chem 73:1053
Mayr T, Klimant I, Wolfbeis OS, Werner T (2002) Anal Chim Acta 462:1
Borisow SM, Krause C, Arain S, Wolfbeis OS (2006) Adv Mater 18:1511
Vasylevska GS, Borisov SM, Krause C, Wolfbeis OS (2006) Chem Mater 18:4609
Vedvik KL, Eliason HC, Hoffmann RL, Gibson JR, Kupcho KR, Somberg RL, Vogel KW (2004) Assay Drug Dev Technol 2:193
Latif S, Bauer-Sardina I, Ranade K, Livak KJ, Kwok PY (2001) Genome Res 11:436
Murakami A, Nakaura M, Naktatsuji Y, Nagahara S, Tran-Cong Q, Makino K (1991) Nucleic Acids Res 19:4097
Crane BL, Hogan NC, Sudo H, Thilly WG, Hunter IW (2005) Anal Chem 77:5129
Yishai Y, Fixler D, Cohen-Kashi M, Zurgil N, Deutsch M (2003) Phys Med Biol 48:2255
Augustin CM, Wolfbeis OS (2003) J Mol Liq 107:141
Lakowicz JR (1999) Principles of Fluorescence Spectroscopy, 2nd edn. Kluwer Academic/Plenum, New York, pp 291–304
Terpetschnig E, Szmacinski H, Malak H, Lakowicz JR (1995) Biophys J 68:342
Guo X-Q, Castellano FN, Li L, Lakowicz JR (1998) Anal Chem 70:632
Perrin F (1926) J Phys Radium 7:390
Jabłonsky A (1960) Bull Acad Pol Sci 8:529
Kawski A (1993) Crit Rev Anal Chem 23:459
Steiner RF (1991) In: Lakowicz JR (ed) Topics in Fluorescence Spectroscopy, vol 2, Principles. Plenum, New York, pp 1–52
Lapari G, Szabo A (1980) Biophys J 30:489
Lentz BR (1989) Chem Phys Lipid 50:171
Neilson RDM, Soutar I, Steedman W (1977) Macromolecules 10:1193
Cogan U, Shinitzky M, Weber G, Nishida T (1973) Biochemistry 12:521
Nobbs JH, Bower DI, Ward IM, Patterson D (1974) Polymer 15:287
Scust J, Berg T (2004) Anal Biochem 330:114
McCauley TG, Hamaguchi N, Stanton M (2003) Anal Biochem 319:244
Blommel P, Hanson GT, Vogel KW (2004) J Biomol Screen 9:294
Webb WW (1976) Quart Rev Biophys 9:49
Gadella TWJ, Clegg RM, Jovin TM (1994) Bioimaging 2:139
Van Munster EB, Gadella TWJ (2004) Cytometry A 58:185
Thompson RL (1991) Correlation Spectroscopy. In: Lakowicz JR (ed) Topics in Fluorescence Spectroscopy, vol 1, Techniques. Plenum, New York, pp 337–378
Madge D (1976) Quart Rev Biophys 9:35
Charier S, Meglio A, Alcor D, Cogne-Laage E, Allemand J-F, Julien L, Lemarchand A (2005) J Am Chem Soc 127:15491
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Schäferling, M., Duerkop, A. (2008). Intrinsically Referenced Fluorimetric Sensing and Detection Schemes: Methods, Advantages and Applications. In: Resch-Genger, U. (eds) Standardization and Quality Assurance in Fluorescence Measurements I. Springer Series on Fluorescence, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/4243_2008_023
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