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Advanced Technologies for Studies on Protein Interactomes

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Protein – Protein Interaction

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 110))

Abstract

One of the key challenges of biology in the post-genomic era is to assign function to the many genesrevealed by large-scale sequencing programmes, since only a small fraction of gene function can bedirectly inferred from the coding sequence. Identifying interactions between proteins is a substantialpart in understanding their function. The main technologies for investigating protein–protein interactionsand assigning functions to proteins include direct detection intermolecular interactions through proteinmicroarray, yeast two-hybrid system, mass spectrometry fluorescent techniques to visualize protein complexesor pull-down assays, as well as technologies detecting functional interactions between genes, such as RNAiknock down or functional screening of cDNA libraries. Over recent years, considerable advances have beenmade in the above techniques. In this review, we discuss some recent developments and their impact on thegene function annotation.

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  1. Cardiovascular Research Unit, University of Sheffield, Royal Hallamshire Hospital, Glossop road, S10 2JF, Sheffield, UK

    Hongtao Guan & Endre Kiss-Toth

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  1. Hongtao Guan
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Correspondence to Endre Kiss-Toth .

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Meike Werther Harald Seitz

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Guan, H., Kiss-Toth, E. (2008). Advanced Technologies for Studies on Protein Interactomes. In: Werther, M., Seitz, H. (eds) Protein – Protein Interaction. Advances in Biochemical Engineering/Biotechnology, vol 110. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_092

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