Abstract
The identification of protein interaction partners can often elucidate the function of the proteinunder investigation based on the “guilty by association” concept. Furthermore, the bindingevent between two proteins can be used as a functional assay when no such assay is available. Despitethe large number of advanced techniques that are currently available for studying protein–proteininteractions, far-Westerns or blot overlays are still very commonly used in the average laboratory settingdue to their powerfulness. This is due to the simplicity and clarity in the results that they produce.Here, the details and mechanics of far-Westerns are discussed to help the reader choose amongst the differentvariations that exist depending on the question being investigated and the materials available to them.Some examples involving unique questions are also discussed in order to educate the reader on the versatilityof far-Westerns. Finally, a troubleshooting section provides the reader with an understanding of thecommon problems that can be encountered and how these problems can be circumvented.
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Chan, C.S., Winstone, T.M.L., Turner, R.J. (2008). Investigating Protein–Protein Interactions by Far-Westerns. In: Werther, M., Seitz, H. (eds) Protein – Protein Interaction. Advances in Biochemical Engineering/Biotechnology, vol 110. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2007_090
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DOI: https://doi.org/10.1007/10_2007_090
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