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Preliminary Study of Simultaneous Multi-Anticoagulant Deficiency Diagnosis by Fiber Optic Multi-Analyte Biosensor

  • Liang Tang
  • Kyung A. Kang
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)

Abstract

Protein C (PC), protein S (PS), antithrombin III, and plasminogen are four important anticoagulants in blood plasma. Deficiency of any of these biomolecules may lead to thrombo-embolic complications including lung embolism, heart attack, and stroke. A multi-factor sensing system is beneficial for identifying the cause of abnormal blood clotting more effectively, rapidly, and cost-effectively. As an initial effort toward simultaneous multi-anticoagulant detection, a PC and PS dual-sensing system has been under development in our research group.

A fiberoptic PC biosensor utilizing fluorophore-mediated sandwich immunoassay was already developed for rapid (∼5 minutes) PC deficiency diagnosis. After a single PS sensor was developed for the PS deficiency diagnosis, the two sensors were connected in series to form a dual-sensing system. The cross-reactivity between the analytes and the sensors was found to be minimal. For easier sensing operation, a mixture of fluorophore-linked anti-PC and anti-PS was applied. The results showed that the mixture can be used with a slight signal reduction. When PC and PS was mixed in a sample, the signal intensity was decreased by approximately 5% for both sensors. A study is currently being performed to overcome the signal reduction by increasing the flow velocity and incubation time.

Keywords

Hemostatic Defect Deficiency Diagnosis Simultaneous Protein Human Factor Versus Optic Biosensing System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Liang Tang
  • Kyung A. Kang

There are no affiliations available

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