Preliminary Study of Simultaneous Multi-Anticoagulant Deficiency Diagnosis by Fiber Optic Multi-Analyte Biosensor

  • Liang Tang
  • Kyung A. Kang
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 566)


Protein C (PC), protein S (PS), antithrombin III, and plasminogen are four important anticoagulants in blood plasma. Deficiency of any of these biomolecules may lead to thrombo-embolic complications including lung embolism, heart attack, and stroke. A multi-factor sensing system is beneficial for identifying the cause of abnormal blood clotting more effectively, rapidly, and cost-effectively. As an initial effort toward simultaneous multi-anticoagulant detection, a PC and PS dual-sensing system has been under development in our research group.

A fiberoptic PC biosensor utilizing fluorophore-mediated sandwich immunoassay was already developed for rapid (∼5 minutes) PC deficiency diagnosis. After a single PS sensor was developed for the PS deficiency diagnosis, the two sensors were connected in series to form a dual-sensing system. The cross-reactivity between the analytes and the sensors was found to be minimal. For easier sensing operation, a mixture of fluorophore-linked anti-PC and anti-PS was applied. The results showed that the mixture can be used with a slight signal reduction. When PC and PS was mixed in a sample, the signal intensity was decreased by approximately 5% for both sensors. A study is currently being performed to overcome the signal reduction by increasing the flow velocity and incubation time.


Hemostatic Defect Deficiency Diagnosis Simultaneous Protein Human Factor Versus Optic Biosensing System 
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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Liang Tang
  • Kyung A. Kang

There are no affiliations available

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