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Increasing Monoclonal Antibody Productivity by Semicontinuous Substitution of Production Medium for Growth Medium

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New Developments and New Applications in Animal Cell Technology
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References

  1. Griffiths, IB. (1992) Animal cell culture processes-batch or continuous. J. Biotechnol. 22, 21–30.

    Article  CAS  PubMed  Google Scholar 

  2. Büntemayer, H., Wallerius, C., and Lehmann, J. (1992) Optimal medium use for continuous high density perfusion processes. Cytotechnology 9, 59–67.

    Google Scholar 

  3. Pörtner, R., Lüdemann, I. and Märkl, H. (1997) Dialysis cultures with immobilized hybridoma cells for effective production of monoclonal antibodies. Cytotechnology 23, 39–45.

    Article  Google Scholar 

  4. Hiller, G.W., Clark, D.S. and Blanch, H.W. (1993) Cell-retention-chemostat studies of hybridoma cells: Analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol. Bioeng. 42, 185–195.

    Article  CAS  Google Scholar 

  5. Franěk F. (1995) Starvation-induced programmed death of hybridoma cells: Prevention by amino acid mixtures. Biotechnol. Bioeng. 45, 86–90.

    Google Scholar 

  6. Franěk F., and Šrámková K. (1997) Unbalanced media for hybridoma cell culture: an alternative reality, in M.J.T. Carrondo et al. (eds.), Animal Cell Technology. From Vaccines to Genetic Medicine, Kluwer Academic Publishers, Dordrecht, pp. 675–680.

    Google Scholar 

  7. Franěk, F., and Chládková-Šrámková K. (1995) Apoptosis and nutrition: Involvement of amino acid transport system in repression of hybridoma cell death. Cytotechnology 18, 113–117.

    Google Scholar 

  8. Franěk, F. and Šrámková K. (1996) Cell suicide in starving hybridoma culture: survival-signal effect of some amino acids. Cytotechnology 21, 81–89.

    Google Scholar 

  9. Franěk, F., and Šrámková K. (1996) Protection of B lymphocyte hybridoma against starvation-induced apoptosis: survival-signal role of some amino acids, Immunol. Lett. 52, 139–144.

    PubMed  Google Scholar 

  10. Franěk, F., and Šrámková K. (1997) Amino acid signaling for growth or death, in K. Funatsu et al. (eds.), Animal Cell Technology: Basic & Applied Aspects, Vol.8, Kluwer Academic Publishers, Dordrecht, pp. 131–135.

    Google Scholar 

  11. Franěk, F., Vomastek, T. and Dolníková, J. (1992) Fragmented DNA and apoptotic bodies document the programmed way of cell death in hybridoma cultures. Cytotechnology 9, 117–123.

    PubMed  Google Scholar 

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Otto-Wilhelm Merten Pierre Perrin Bryan Griffiths

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© 1998 Kluwer Academic Publishers

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Franěk, F., Šrámková, K. (1998). Increasing Monoclonal Antibody Productivity by Semicontinuous Substitution of Production Medium for Growth Medium. In: Merten, OW., Perrin, P., Griffiths, B. (eds) New Developments and New Applications in Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/0-306-46860-3_9

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  • DOI: https://doi.org/10.1007/0-306-46860-3_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5016-3

  • Online ISBN: 978-0-306-46860-5

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