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Characterization of Scaptotrigona mexicana Pot-Pollen from Veracruz, Mexico

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Pot-Pollen in Stingless Bee Melittology

Abstract

The stingless bee Scaptotrigona mexicana is distributed from Mexico to Costa Rica. In Mexico, this species is often found in wet lowlands but more commonly in forests not higher than 1000 m in the states of Tamaulipas, San Luis Potosí, Veracruz, Hidalgo, Puebla, State of Mexico, Morelos, Guerrero, Oaxaca, and Chiapas. This bee commonly nests in tree cavities and is kept in wood or ceramic clay hives by traditional stingless beekeepers. Pot-pollen from three hives managed in central Veracruz, Mexico, at Cañada Blanca, Manuel León, and Fortín de las Flores was analyzed in duplicates. Chemical parameters including ash, proteins, electrical conductivity, pH, phosphorus, and potassium were similar among pot-pollen samples, whereas the highest water and total soluble sugars were in samples from Fortín de las Flores. The lowest lipid content occurred in Cañada Blanca pot-pollen. Although botanical origin of the pot-pollen differed among sites, the number of plant species was nearly the same, with 13 found in Cañada Blanca and Fortín de las Flores and 11 at Manuel León. Most pollen types belonged to Tiliaceae and Burseraceae in Cañada Blanca, Fabaceae and Asteraceae in Manuel León, and Solanaceae and Asteraceae in Fortín de las Flores.

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References

  • Albores-González ML, García-Guerra TG, Durán-Olguín L, Aguliar-Ayón A. 2011. Experiencia de la Unión de Cooperativas Tosepan en el fomento a la cría de las abejas nativas Pitsilnejmej (Scaptotrigona mexicana). pp. 95-99. In: Memorias del VII Seminario Mesoamericano sobre Abejas Nativas. San Cristóbal de las Casas, Chiapas, México. 242 p.

    Google Scholar 

  • Almeida-Muradian LB, Pamplona LC, Coimbra S, Barth OM. 2005. Chemical composition and botanical evaluation of dried bee pollen pellets. Journal of Food Composition and Analysis 18: 105-111. DOI:10.1016/j.jfca.2003.10.008

    Article  CAS  Google Scholar 

  • Almeida-Muradian LB, Stramm KM, Horita A, Barth OM, da Silva de Freitas A, Estevinho LM. 2013. Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera. International Journal of Food Science and Technology 48: 1698-1706. DOI:10.1111/ijfs.12140

    Article  CAS  Google Scholar 

  • Anderson KE, Sheehan TH, Eckholm BJ, Mott BM, DeGrandi-Hoffman G. 2011. An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux 58: 431-444. DOI:10.1007/s00040-011-0194-6

    Article  Google Scholar 

  • AOAC. 1996. Official methods of analysis of the Association of Official Analytical Chemists. Association of Official Analytical Chemists. Arlington, VA, USA. 937 pp.

    Google Scholar 

  • Arzaluz A, Obregón F, Jones R. 2002. Optimum brood size for artificial propagation of the stingless bee Scaptotrigona mexicana. Journal of Apicultural Research 41: 62-63. DOI:10.1080/00218839.2002.11101070

    Article  Google Scholar 

  • Ayala R. 1999. Revisión de las abejas sin aguijón de México (Hymenoptera: Apidae: Meliponini). Folia Entomológica Mexicana 106: 1-123.

    Google Scholar 

  • Ayala R, González V, Engel M. 2013. Mexican stingless bees (Hymenoptera: Apidae): diversity, distribution, and indigenous knowledge. pp. 135-152. In Vit P, Pedro-Silvia RM, Roubik D, eds. Pot-Honey: A Legacy of Stingless Bees. Springer; New York, NY, USA. 654 pp.

    Google Scholar 

  • Barajas J, Cortes-Rodriguez M, Rodríguez-Sandoval E. 2012. Effect of temperature on the drying process of bee pollen from two zones of Colombia. Journal of Food Process Engineering 35: 134-148. DOI:10.1111/j.1745-4530.2010.00577.x

    Article  Google Scholar 

  • Camargo JMF, Pedro SRM. 2013. Meliponini Lepeletier, 1836. In Moure JS, Urban D, Melo GAR, orgs. Catalogue of bees (Hymenoptera, Apoidea) in the Neotropical Region – online version. Available at: http://moure.cria.org.br/catalogue?id=34932

  • Campos MGR, Bogdanov S, De Almeida-Muradian LB, Szczesna T, Mancebo Y, Frigerio C, Ferreira F. 2008. Pollen composition and standardisation of analytical methods. Journal of Apicultural Research 47: 154-161. DOI:10.3896/IBRA.1.47.2.12

    Article  CAS  Google Scholar 

  • Cano-Contreras EJ, Martínez-Martínez C, Balboa-Aguilar CC. 2013. La “Abeja de Monte” (Insecta: Apidae, Meliponini) de los Choles de Tacotalpa, Tabasco: Conocimiento local, presente y futuro. Etnobiología 11: 47-57.

    Google Scholar 

  • Cimpoiu C, Hosu A, Miclaus V, Puscas A. 2013. Determination of the floral origin of some Romanian honeys on the basis of physical and biochemical properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 100: 149-154. DOI:10.1016/j.saa.2012.04.008

    Article  CAS  Google Scholar 

  • Codex Alimentations. 2001. Draft revised standard for standard for honey (at step 10 of the Codex procedure). Alinorm 01/25 (2001). pp. 19-26.

    Google Scholar 

  • Cornman RS, Otto CR, Iwanowicz D, Pettis JS. 2015. Taxonomic characterization of honey bee (Apis mellifera) pollen foraging based on non-overlapping paired-end sequencing of nuclear ribosomal loci. PLoS One 10: e0145365. DOI:10.1371/journal.pone.0145365

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cortopassi-Laurino M, Imperatriz-Fonseca VL, Roubik DW, Dollin A, Heard T, Aguilar I, Venturieri GC, Eardley C, Nogueira-Neto P. 2006. Global meliponiculture: challenges and opportunities. Apidologie 37: 275-292. DOI:10.1051/apido:2006027

    Article  Google Scholar 

  • Da Costa Leite JM, Trugo LC, Costa LSM, Quinteiro LMC, Barth OM, Dutra VML. 2000. Determination of oligosaccharides in Brazilian honeys of different botanical origin. Food Chemistry 70:93-98. DOI:10.1016/S0956-7135(99)00115-2

    Article  Google Scholar 

  • Dardón MJ, Maldonado-Aguilera C, Enríquez E. 2013. The Pot-Honey of Guatemalan Bees. pp. 395-408. In: Vit P, Pedro-Silvia RM, Roubik D, eds. Pot-Honey: A Legacy of Stingless Bees. Springer; New York, NY, USA. 654 pp.

    Google Scholar 

  • Decourtye A, Mader E, Desneux N. 2010. Landscape enhancement of floral resources for honey bees in agro-ecosystems. Apidologie 41:264-277. DOI:10.1051/apido/2010024

    Article  Google Scholar 

  • Di Pasquale G, Salignon M, Le Conte Y, Belzunces LP, Decourtye A, Kretzschmar A, Suchail S, Brunet JL, Alaux C. 2013. Influence of pollen nutrition on honey bee health: Do pollen quality and diversity matter? PLoS ONE 8: e72016. DOI:10.1371/journal.pone.0072016

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Domínguez-Valhondo D, Bohoyo Gil D, Hernández MT, González-Gómez D. 2011. Influence of the commercial processing and floral origin on bioactive and nutritional properties of honeybee-collected pollen. International Journal of Food Science and Technology 46: 2204-2211. DOI:10.1111/j.1365-2621.2011.02738.x

    Article  CAS  Google Scholar 

  • El Sohaimy SA, Masry SHD, Shehata MG. 2015. Physicochemical characteristics of honey from different origins. Annals of Agricultural Sciences 60: 279-287. DOI:10.1016/j.aoas.2015.10.015

    Article  Google Scholar 

  • Eltz T, Brühl CA, Van der Kaars S, Linsenmair KE. 2001. Assessing stingless bee pollen diet by analysis of garbage pellets: a new method. Apidologie 32:341-353. DOI:10.1051/apido:2001134

    Article  Google Scholar 

  • Erdtman, G. 1969. Handbook of Palynology (Morphology-Taxonomy-Ecology). An Introduction to the Study of Pollen Grains and Spores. Munksgaard, Copenhagen. 486 pp.

    Google Scholar 

  • Estevinho LM, Rodrigues S, Pereira AP, Feás X. 2012. Portuguese bee pollen: palynological study, nutritional and microbiological evaluation. International Journal of Food Science and Technology 47: 429-435. DOI:10.1111/j.1365-2621.2011.02859.x

    Article  CAS  Google Scholar 

  • Feás X, Vázquez-Tato MP, Estevinho L, Seijas JA, Iglesias A. 2012. Organic bee pollen: botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality. Molecules 17:8359-8377. DOI:10.3390/molecules17078359

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • García-Flores A, del Amo Rodríguez S, Hernández-Colorado MR. 2013. Taxkat, la abeja nativa de Mesoamérica. La Ciencia y el Hombre 26: Enero-Abril 2013. Available at: https://www.uv.mx/cienciahombre/revistae/vol26num1/articulos/las-abejas.html

  • González-Acereto J. 2008. Cría y manejo de abejas nativas sin aguijón en México. Universidad Autónoma de Yucatán. Planeta Impresores. Mérida, Yucatán, México. 177 pp.

    Google Scholar 

  • Grembecka M, Szefer P. 2013. Evaluation of honeys and bee products quality based on the mineral composition using multivariate techniques. Environmental Monitoring and Assessment 185: 4033-4047. DOI:10.1007/s10661-012-2847-y

    Article  PubMed  CAS  Google Scholar 

  • Guzmán M, Balboa C, Vandame R, Albores MA, González-Acereto J. 2011. Manejo de las abejas nativas sin aguijón en México Melipona beecheii y Scaptotrigona mexicana. El Colegio de la Frontera Sur. 64 p. Available at: file:///C:/Users/Usuario/Downloads/ECO%20Manual%20meliponicultura%202011ecosur%20(1).pdf

    Google Scholar 

  • Hurtado-Burillo M, Ruiz C, May-Itzá WJ, Quezada-Eúan JJG, de la Rúa P. 2013. Barcoding stingless bees: genetic diversity of the economically important genus Scaptotrigona in Mesoamerica. Apidologie 44:1-10. DOI:10.1007/s13592-012-0146-9

    Article  Google Scholar 

  • Hurtado-Burillo M. 2015. Caracterización molecular y morfométrica del género Scaptotrigona (Apidae: Meliponini) en Mesoamérica. Tesis de Doctorado. Universidad de Murcia-Facultad de Biología. Murcia, España. 167 p. Available at: http://www.tesisenred.net/bitstream/handle/10803/349217/TMHB.pdf?sequence=1

  • Kaškonienė V, Venskutonis PR, Čeksterytė V. 2010. Carbohydrate composition and electrical conductivity of different origin honeys from Lithuania. LWT – Food Science and Technology 43: 801-807. DOI:10.1016/j.lwt.2010.01.007

    Article  CAS  Google Scholar 

  • Kostić AŽ, Barać MB, Stanojević SP, Milojković-Opsenica DM, Tešić ŽL, Šikoparija B, Radišić P, Prentović M, Pešić MB. 2015. Physicochemical composition and techno-functional properties of bee pollen collected in Serbia. LWT – Food Science and Technology 2015:1-9. DOI:10.1016/j.lwt.2015.01.031

    Article  CAS  Google Scholar 

  • Krell R. 1996. Value-added products from beekeeping. FAO Agricultural Services Bulletin No. 124. Food and Agriculture Organization of the United Nations. Rome, Italy. Available at: http://www.fao.org/docrep/w0076e/w0076e00.htm

  • Kroyer G, Hegedus N. 2001. Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innovative Food Science and Emerging Technologies 2: 171-174. DOI:10.1016/S1466-8564(01)00039-X

    Article  CAS  Google Scholar 

  • Leonhardt SD, Dworschak K, Eltz T, Blüthgen N. 2007. Foraging loads of stingless bees and utilisation of stored nectar for pollen harvesting. Apidologie 38:125-135. DOI:10.1051/apido:2006059

    Article  CAS  Google Scholar 

  • Menezes C, Vollet-Neto A, Imperatriz-Fonseca VL. 2012. A method for harvesting unfermented pollen from stingless bees (Hymenoptera, Apidae, Meliponini). Journal of Apicultural Research 51:240-244. DOI:10.3896/IBRA.1.51.3.04

    Article  Google Scholar 

  • Menezes C, Vollet-Neto A, León-Contrera FA, Venturieri GC, Imperatriz-Fonseca VL. 2013. The role of useful microorganisms to stingless bees and stingless beekeeping. pp. 153-171. In: Vit P, Pedro SRM, Roubik D, eds. Pot-Honey: A legacy of stingless bees. Springer. New York, NY, USA. 654 p.

    Chapter  Google Scholar 

  • Mueller MY, Moritz RF, Kraus FB. 2012. Outbreeding and lack of temporal genetic structure in a drone congregation of the Neotropical stingless bee Scaptotrigona mexicana. Ecology and Evolution 2: 1304-1311. DOI: 10.1002/ece3.203

    Article  PubMed  PubMed Central  Google Scholar 

  • Nogueira C. Iglesias A, Feas X, Estevinho LM. 2012. Commercial bee pollen with different geographical origins: a comprehensive approach. International Journal of Molecular Sciences 13: 11173-11187. DOI:10.3390/ijms130911173

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Padilla-Vargas PJ, Vásquez-Dávila MA, García-Guerra TG, Albores-González ML. 2014. Pisilnekmej: una mirada a la cosmovisión, conocimientos y prácticas nahuas sobre Scaptotrigona mexicana en Cuetzalan, Puebla, México. Etnoecológica 10:37-40.

    Google Scholar 

  • Palacios-Chávez R, Ludlow-Wiechers B, Villanueva-Gutiérrez R. 1991. Flora palinológica de la Reserva de la Biosfera de Sian ka’an, Quintana Roo, México. Centro de Investigaciones de Quintana Roo. Chetumal, Quintana Roo, Mexico. 321 pp.

    Google Scholar 

  • Palmer KA, Oldroyd BP, Quezada-Euán JJ, Paxton RJ, May-Itza Wde J. 2002. Paternity frequency and maternity of males in some stingless bee species. Molecular Ecology 11:2107-2113.

    Article  CAS  PubMed  Google Scholar 

  • Pascoal A, Rodrigues S, Teixeira A, Feás X, Estevinho LM. 2014. Biological activities of commercial bee pollens: antimicrobial, antimutagenic, antioxidantand anti-inflammatory. Food and Chemical Toxicology 63:233-239. DOI:10.1016/j.fct.2013.11.010

    Article  PubMed  CAS  Google Scholar 

  • Patlán-Martínez E, Kañetas OKJ, Guerrero FH, López MS. 2015. Las abejas nativas: tradición totonaca en el cuidado de la Naturaleza. pp. 1525-1530. In Memorias del V Congreso Latinoamericano de Agroecología. La Plata, Argentina.

    Google Scholar 

  • Rebelo KS, Ferreira AG, Carvalho-Zilse GA. 2016. Physicochemical characteristics of pollen collected by Amazonian stingless bees. Ciencia Rural 46. DOI:10.1590/0103-8478cr20150999

    Article  Google Scholar 

  • Reyes-González A, Camou-Guerrero A, Reyes-Salas O, Argueta A, Casas A. 2014. Diversity, local knowledge and use of stingless bees (Apidae: Meliponini) in the municipality of Nocupétaro, Michoacán, Mexico. Journal of Ethnobiology and Ethnomedicine 10: 47. DOI:10.1186/1746-4269-10-47

    Article  PubMed  PubMed Central  Google Scholar 

  • Rosa CA, Lachance MA, Silva JOC, Teixeira ACP, Marini MM, Antonini Y, Martins RP. 2003 Yeast communities associated with stingless bees. FEMS Yeast Research 4:271-275. DOI:10.1016/S1567-1356(03)00173-9

    Article  PubMed  CAS  Google Scholar 

  • Rosso JML, Imperatriz-Fonseca VL, Cortopassi-Laurino, M. 2001. Meliponicultura en Brasil I: situación en 2001 y perspectivas. pp. 28-35. In Memorias del II Seminario Mexicano sobre Abejas sin Aguijón. Mérida, Yucatán, Mexico. 136 p.

    Google Scholar 

  • Rzepecka-Stojko A, Stojko J, Kurek-Górecka A, Górecki M, Kabała-Dzik A, Kubina R, Moździerz A, Buszman E. 2015. Polyphenols from bee pollen: structure, absorption, metabolism and biological activity. Molecules 20:21732-21749. DOI: 10.3390/molecules201219800.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sánchez D, Nieh JC, Hénaut Y, Cruz L, Vandame R. 2004. High precision during food recruitment of experienced (reactivated) foragers in the stingless bee Scaptotrigona mexicana (Apidae, Meliponini). Naturwissenschaften 91:346-349. DOI:10.1007/s00114-004-0536-6

    Article  PubMed  CAS  Google Scholar 

  • Sánchez D, Vandame R. 2012. Color and shape discrimination in the stingless bee Scaptotrigona mexicana Guérin (Hymenoptera, Apidae). Neotropical Entomology 41: 171-177. DOI:10.1007/s13744-012-0030-3

    Article  PubMed  Google Scholar 

  • Sánchez D, Solórzano-Gordillo E, Vandame R. 2016. A Study on Intraspecific Resource Partitioning in the Stingless bee Scaptotrigona mexicana Guérin (Apidae, Meliponini) Using Behavioral and Molecular Techniques. Neotropical Entomology DOI: 10.1007/s13744-016-0404-z

    Article  PubMed  Google Scholar 

  • Simeão CM, Silveira FA, Sampaio IB, Bastos EM. 2016. Pollen analysis of honey and pollen collected by Apis mellifera Linnaeus, 1758 (Hymenoptera, Apidae), in a mixed environment of Eucalyptus plantation and native cerrado in Southeastern Brazil. Brazilian Journal of Biology 75:821-829. DOI:10.1590/1519-6984.23513.

    Article  Google Scholar 

  • Souza BA, Lopes MTR, Pereira FM. 2012. Cultural aspects of meliponiculture. pp. 1-6. In Vit P, Roubik DW, eds. Stingless bees process honey and pollen in cerumen pots. SABER-ULA. Universidad de Los Andes. Mérida, Venezuela. Available at: http://www.saber.ula.ve/handle/123456789/35619

    Google Scholar 

  • Vit P, Herrera P, Rodríguez D, Carmona J. 2008. Characterization of fresh bee pollen collected in Cacute, in Venezuelan Andes. Revista del Instituto Nacional de Higiene “Rafael Rangel” 39: 7-11.

    Google Scholar 

  • Vit P, Medina M, Enríquez E. 2004. Quality standards for medicinal uses of Meliponinae honey in Guatemala, Mexico and Venezuela. Bee World 85:2-5. DOI: 10.1080/0005772X.2004.11099603

    Article  Google Scholar 

  • Vit P, Santiago B, Pedro SRM, Perez-Perez E, Pena-Vera M. 2016. Chemical and bioactive characterization of pot-pollen produced by Melipona and Scaptotrigona stingless bees from Paria Grande, Amazonas State, Venezuela. Emirates Journal of Food and Agriculture 28:78-84. DOI:10.9755/ejfa.2015-05-245

    Article  Google Scholar 

  • Vossler FG. 2015. Broad Protein Spectrum in Stored Pollen of Three Stingless Bees from the Chaco Dry Forest in South America (Hymenoptera, Apidae, Meliponini) and Its Ecological Implications. Psyche 2015: Article ID 659538. DOI:10.1155/2015/659538

    Article  Google Scholar 

  • Yang K, Wu D, Ye X, Liu D, Chen J, Sun P. 2013. Characterization of chemical composition of bee pollen in China. Journal of Agricultural and Food Chemistry 61: 708-718. DOI:10.1021/jf304056b

    Article  PubMed  CAS  Google Scholar 

  • Yáñez-Ordóñez O, Trujano-Ortega M, Llorente-Bousquets J. 2008. Patrones de distribución de las especies de la tribu Meliponini (Hymenoptera: Apoidea: Apidae) en México. Interciencia 33:41–45.

    Google Scholar 

  • Ziska LH, Pettis JS, Edwards J, Hancock JE, Tomecek MB, Clark A, Dukes JS, Loladze I, Polley HW. 2016. Rising atmospheric CO2 is reducing the protein concentration of a floral pollen source essential for North American bees. Proceedings of the Royal Society B: Biological Sciences 283. pii: 20160414. DOI:10.1098/rspb.2016.0414

    Article  PubMed  PubMed Central  CAS  Google Scholar 

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Acknowledgments

We acknowledge the Mexico’s National Council of Science and Technology (CONACYT) for the M.Sc. scholarship No. 372038 granted to LALG. ACO also thanks the financial support from Colegio de Postgraduados through the Management and Investment Trust No. 167304. We are especially thankful to Dr. David W. Roubik for his helpful comments and suggestions; his expert advice and constructive criticism have been invaluable in ensuring the most careful scrutiny, which certainly served to improve this chapter substantially. We also are very much grateful to Dr. Patricia Vit, not only for her work as an editor but also for her sustained encouragement, interest, support, and valuable advice throughout the editorial process.

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Contreras-Oliva, A. et al. (2018). Characterization of Scaptotrigona mexicana Pot-Pollen from Veracruz, Mexico. In: Vit, P., Pedro, S., Roubik, D. (eds) Pot-Pollen in Stingless Bee Melittology. Springer, Cham. https://doi.org/10.1007/978-3-319-61839-5_23

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