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Classical Conditioning in Aplysia: Neuronal Circuits Involved in Associative Learning

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Conditioning

Part of the book series: Advances in Behavioral Biology ((ABBI,volume 26))

Summary

Recent analyses of the neural control of learning and memory suggest that one needs to identify and examine the neuronal circuitry specific to the behavior that is modified by the learning in order to study the cellular mechanisms underlying these processes. In this paper we describe an example of a simple form of classical conditioning in the gastropod mollusk Aplysia californica. This example of associative learning offers considerable promise for a cellular analysis because it involves relatively simple and well-analyzed neuronal circuits. Knowledge of the neuronal circuits involved in the conditioning pathways is reviewed, and a preliminary hypothesis for the mechanisms of this form of classical conditioning is considered.

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References

  • Bailey, C.H., Castellucci, V.F., Koester, J., and Kandel, E.R., 1979, Cellular studies of peripheral neurons in siphon skin of Aplysia californica, J. Neurophysiol. 42:530.

    PubMed  CAS  Google Scholar 

  • Brunelli, M., Castellucci, V., and Kandel, E.R., 1976, Presynaptic facilitation as a mechanism for behavioral sensitization in Aplysia, Science 194:1176.

    Article  Google Scholar 

  • Byrne, J.H., and Koester, J., 1978, Respiratory pumping: Neuronal control of a centrally commanded behavior in Aplysia, Brain Res., 143:87.

    Article  PubMed  CAS  Google Scholar 

  • Byrne, J.H. Castellucci, V., and Kandel, E.R., 1974, Receptive fields and response properties of mechanoreceptor neurons innervating siphon skin and mantle shelf in Aplysia, J. Neurophysiol. 37:1041.

    PubMed  CAS  Google Scholar 

  • Byrne, J.H., Castellucci, V., and Kandel, E.R., 1978, Contribution of individual mechanoreceptor sensory neurons to defensive gill-withdrawal reflex in Aplysia, J. Neurophysiol. 41:418.

    PubMed  CAS  Google Scholar 

  • Camardo, J.S., Klein, M., and Kandel, E.R., 1981, Sensitization irk Aplysia: Serotonin elicits a decrease in sensory neuron K current not related to IK early or IK Ca++, Soc. Neurosci. Abstr. 7:836.

    Google Scholar 

  • Carew, T.J., Walters, E.T., and Kandel, E.R., 1981a, Associative learning in Aplysia: Cellular correlates supporting a conditioned fear hypothesis, Science 211: 501.

    CAS  Google Scholar 

  • Carew, T.J., Walters, E.T., and Kandel, E.R., 1981b, Associative learning in a simple reflex in Aplysia, Soc. Neurosci. Abstr. 7:353.

    Google Scholar 

  • Carew, T.J., Walters, E.T., and Kandel, E.R., 1981c, Classical conditioning in a simple withdrawal reflex in Aplysia californica, J. Neurosci. in press.

    Google Scholar 

  • Carew, T.J. and Kandel, E.R., 1982, Some conceptual issues in the study of simple forms of learning, in Princeton Symposium on Primary Neural Substrates of Learning and Behavior Change in preparation.

    Google Scholar 

  • Castellucci, V.F. and Kandel, E.R., 1974, A quantal analysis of the synaptic depression underlying habituation of the gill-withdrawal reflex in Aplysia, Proc. Natl. Acad. Sci. USA, 71:5004.

    Google Scholar 

  • Castellucci, V.F. and Kandel, E.R., 1976, Presynaptic facilitation as a mechanism for behavioral sensitization in Aplysia, Science 194:1176.

    Google Scholar 

  • Castellucci, V.F., Pinsker, H., Kupfermann, H.I., and Kandel, E.R., 1970, Neuronal mechanisms of habituation and dishabituation of the gill-withdrawal reflex in Aplysia, Science 167:1745.

    Google Scholar 

  • Castellucci, V., Schwartz, J.H., Kandel, E.R., Nairn, A., and Greengard, P., 1981, Protein inhibitor of the cyclic AMP-dependent protein kinase can block the onset of, as well as reverse the electrophysiological correlates of sensitization of the gill-withdrawal in Aplysia Soc. Neurosci. Abstr. 7:836.

    Google Scholar 

  • Chang, J.J. and Gelperin, A., 1980, Rapid taste aversion learning by an isolated molluscan central nervous system, Proc. Natl. Acad. Sci. USA 77:6204.

    Google Scholar 

  • Crow, T. and Alkon, D.L., 1978, Retention of an associative behavioral change in Hermissenda, Science 201:1239.

    Google Scholar 

  • Crow, T.J. and Alkon, D.L., 1980, Associative behavioral modification in Hermissenda: Cellular correlates, Science 209: 412.

    CAS  Google Scholar 

  • Davis, W.J. and Gillette, R., 1978, Neural correlate of behavioral plasticity in command neurons of Pleurobranchaea, Science 199:801.

    Google Scholar 

  • Davis, W.J., Villet, J., Lee, D., Rigler, M., Gillette, R., and Prince, E., 1980, Selctive and differential avoidance learning in the feeding and withdrawal behavior of Pleurobranchaea californica, J. Comp. Physiol. A 138:157.

    Google Scholar 

  • Frazier, W.T., Kandel, E.R., Kupfermann, I., Waziri, R., and Coggeshall, R.E., 1967, Morphological and functional properties of identified neurons in the abdominal ganglion of Aplysia californica, J. Neurophysiol. 30:1288.

    Google Scholar 

  • Gelperin, A., 1975, Rapid food-aversion learning by a terrestrial mollusk, Science 189: 567.

    CAS  Google Scholar 

  • Hawkins, R.D., 1981, Identified facilitating neurons are excited by cutaneous stimuli used in sensitization and classical conditioning of Aplysia, Soc. Neurosci. Abstr. 7:354.

    Google Scholar 

  • Hawkins, R.D., Castellucci, V.F., and Kandel, E.R., 1981a, Interneurons involved in mediation and modulation of the gill-withdrawal reflex in Aplysia. I. Identification and characterization, J. Neurophysiol. 45:304.

    Google Scholar 

  • Hawkins, R.D., Castellucci, V.F., and Kandel, E.R., 1981b, Interneurons involved in mediation and modulation of the gill-withdrawal reflex in Aplysia. II. Identified neurons produce heterosynaptic facilitation contributing to behavioral sensitization, J. Neurophysiol. 45:315.

    Google Scholar 

  • Kandel, E.R., 1967, Cellular studies of learning, in: “The Neurosciences: A Study Program,” G.C. Quarton, T. Melnechuk, F.O. Schmitt, ed., Rockefeller Univ. Press, N.Y.

    Google Scholar 

  • Kandel, E.R., 1976, “Cellular Basis of Behavior,” Freeman and Co., San Francisco.

    Google Scholar 

  • Kandel, E.R. and Schwartz, J.H., 1981, Molecular biology of an elementary form of learning in Aplysia: Modulation of transmitter release by cyclic nucleotides, Science in press.

    Google Scholar 

  • Kanz, J.E., Eberly, L.B., Cobbs, J.S., and Pinsker, H.M., 1979, Neuronal correlates of siphon withdrawal in freely-behaving Aplysia, J. Neurophysiol. 42:1538.

    Google Scholar 

  • Kennedy, D. and Davis, W.J., 1977, Organization of invertebrate motor systems, in “Handbook of Physiology: The Nervous System I,” E.R. Kandel, ed., Williams and Wilkins, Baltimore.

    Google Scholar 

  • Klein, M. and Kandel, E.R., 1978, Presynaptic modulation of voltage-dependent Ca++ current: Mechanism for behavioral sensitization in Aplysia californica, Proc. Natl. Acad. Sci. USA, 75:3512.

    Google Scholar 

  • Klein, M. and Kandel, E.R., 1980, Mechanism of calcium current modulation underlying presynaptic facilitation and behavioral sensitization in Aplysia, Proc. Natl. Acad. Sci. USA, 77:6912.

    Google Scholar 

  • Klein, M. and Kandel, E.R., 1982, On the mechanistic relationship of sensitization to classical conditioning, in Princeton Symposium on Primary Neural Substrates of Learning and Behavioral Change in preparation.

    Google Scholar 

  • Krasne, F.B., 1969, Excitation and habituation of the crayfish escape reflex: The depolarizing response in lateral giant fibres of the isolated abdomen, J. Exp. Biol. 50:29.

    Google Scholar 

  • Kupfermann, I. and Kandel, E.R., 1969, Neuronal controls of a behavioral response mediated by the abdominal ganglion of Aplysia, Science 164:847.

    Google Scholar 

  • Kupfermann, I., Carew, T.J., and Kandel, E.R., 1974, Local, reflexive and central commands controlling gill and siphon movements in Aplysia californica, J. Neurophysiol. 37:990.

    Google Scholar 

  • Lukowiak, K. and Sahley, C., 1981, The in vitro classical conditioning of the gill withdrawal reflex of Aplysia californica, Science 212:1516.

    Google Scholar 

  • Mpitsos, G.J. and Collins, S.D., 1975, Learning: rapid aversion conditioning in the gastropod mollusc Pleurobranchaea, Science 188:954.

    Google Scholar 

  • Perlman, A.J., 1979, Central and peripheral control of siphon-withdrawal reflex in Aplysia californica, J. Neurophysiol. 42:510.

    Google Scholar 

  • Razran, G., 1971, “Mind in Evolution: An East-West Synthesis of Learning Behavior and Cognition,” Houghton Mifflin, Boston.

    Google Scholar 

  • Sahley, C.L., Gelperin, A., and Rudy, J.W., 1981, One-trial associative learning in a terrestrial mollusc, Proc. Natl. Acad. Sci. USA 78:640.

    Google Scholar 

  • Tsukahara, N., 1981, Synaptic plasticity in the mammalian central nervous system, Ann. Rev. Neurosci. 4:351.

    Google Scholar 

  • Walters, E.T., Carew, T.J., and Kandel, E.R., 1979, Associative learning in Aplysia californica, Proc. Natl. Acad. Sci. USA Vol. 76, No. 12:6675.

    Google Scholar 

  • Walters, E.T., Carew, T.J., and Kandel, E.R., 1981a, Associative learning in Aplysia: Evidence for conditioned fear in an invertebrate, Science 211: 504.

    CAS  Google Scholar 

  • Walters, E.T., Carew, T.J., and Kandel, E.R., 1981b, Identification of sensory neurons involved in two forms of classical conditioning in Aplysia, Soc. Neurosci. Abstr. 7:353.

    Google Scholar 

  • Wells, M.J., 1968, “Lower Animals,” McGraw-Hill, New York.

    Google Scholar 

  • Zucker, R.S., 1972, Crayfish escape behavior and central synapses. II. Physiological mechanisms underlying behavioral habituation, J. Neurophysiol. 35:621.

    PubMed  CAS  Google Scholar 

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Walters, E.T., Carew, T.J., Hawkins, R.D., Kandel, E.R. (1982). Classical Conditioning in Aplysia: Neuronal Circuits Involved in Associative Learning. In: Woody, C.D. (eds) Conditioning. Advances in Behavioral Biology, vol 26. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-0701-4_45

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  • DOI: https://doi.org/10.1007/978-1-4757-0701-4_45

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4757-0703-8

  • Online ISBN: 978-1-4757-0701-4

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