Cell Cycle Checkpoint Control Protocols

  • Howard B. Lieberman

Part of the Methods in Molecular Biology™ book series (MIMB, volume 241)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Induction and Detection of Changes in cell Cycle Progression

    1. Front Matter
      Pages 1-1
    2. Howard B. Lieberman, Kevin M. Hopkins
      Pages 3-10
    3. Michael H. Fox
      Pages 11-16
    4. Haiying Hang, Michael H. Fox
      Pages 23-35
    5. Wei-Hsin Sun, Melvin L. DePamphilis
      Pages 37-53
    6. Audra Day, Colette Schneider, Brandt L. Schneider
      Pages 55-76
    7. Eliana B. Gómez, Susan L. Forsburg
      Pages 93-111
  3. Analysis of Genes Involved in Checkpoint Control

    1. Front Matter
      Pages 113-113
    2. Sally A. Amundson, Albert J. Fornace Jr.
      Pages 125-141
    3. Jian Zhang, Lisa Ottmers, Brandt L. Schneider
      Pages 143-161
    4. Todd A. Waldman
      Pages 163-174
    5. Migadlisel Colòn, Nancy C. Walworth
      Pages 175-187
    6. Jian Qin, Zhe Peng, Maureen V. McLeod
      Pages 189-194
    7. Erik K. Flemington, Antonio Rodriguez
      Pages 195-206
    8. Chenguang Wang, Maofu Fu, Richard G. Pestell
      Pages 207-216
  4. Analysis of Proteins Involved in Checkpoint Control

    1. Front Matter
      Pages 217-217

About this book

Introduction

In recent years, cell cycle checkpoints, cellular mechanisms that control the cell cycle and ensure genomic stability, have emerged as significant factors in carcinogenesis and in cancer cells. In Cell Cycle Checkpoint Control Protocols, leading investigators present their best methodologies to probe the mechanisms underlying cell cycle regulation and checkpoint control. Using mammalian, yeast, and frog systems, these experts describe readily reproducible methods to induce cell cycle checkpoints, detect changes in cell cycle progression, identify and analyze genes and proteins that regulate the process, and characterize chromosomal status as a function of cell cycle phase and progression. Each fully tested technique includes step-by-step instructions written by an investigator who performs it frequently, an introduction explaining the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Taken as a whole, the collection describes the major methodologies used by researchers in the field.
Cutting-edge and highly practical, Cell Cycle Checkpoint Control Protocols provides an extensive array of detailed protocols by which both experienced and novice investigators may successfully illuminate questions concerning cell cycle control.

Editors and affiliations

  • Howard B. Lieberman
    • 1
  1. 1.Center for Radiological ResearchColumbia UniversityNew York

Bibliographic information

  • DOI https://doi.org/10.1385/1592596460
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-115-8
  • Online ISBN 978-1-59259-646-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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