Drugs of Abuse

Neurological Reviews and Protocols

  • John Q. Wang

Part of the Methods In Molecular Medicine™ book series (MIMM, volume 79)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Special Reviews

    1. Front Matter
      Pages 1-1
    2. Peter W. Kalivas, Shigenobu Toda, M. Scott Bowers, David A. Baker, M. Behnam Ghasemzadeh
      Pages 3-11
    3. John Q. Wang, Limin Mao, Yuen-Sum Lau
      Pages 33-42
    4. Gary A. Gudelsky, Bryan K. Yamamoto
      Pages 55-73
  3. Detection of MRNA Expression in the Striatum

  4. Detection of Protein Expression in the Striatum

    1. Front Matter
      Pages 261-261

About this book

Introduction

New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting.
State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches.

Editors and affiliations

  • John Q. Wang
    • 1
  1. 1.School of PharmacyUniversity of Missouri-Kansas CityKansasUSA

Bibliographic information

  • DOI https://doi.org/10.1385/1592593585
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-057-1
  • Online ISBN 978-1-59259-358-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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