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E. coli Gene Expression Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Peter E. Vaillancourt

    1. Applied Molecular Evolution, San Diego, USA

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Part of the book series: Methods in Molecular Biology (MIMB, volume 205)

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Table of contents (23 protocols)

  1. Hyperphage

    • Olaf Broders, Frank Breitling, Stefan Dübel
    Pages 295-302

  2. Combinatorial Biosynthesis of Novel Carotenoids in E. coli

    • Gerhard Sandmann
    Pages 303-314

  3. Using Transcriptional-Based Systems for In Vivo Enzyme Screening

    • Steven M. Firestine, Frank Salinas, Stephen J. Benkovic
    Pages 315-327

  4. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis

    • Mary N. Burtnick, Paul J. Brett, Donald E. Woods
    Pages 329-338

  5. Back Matter

    Pages 339-347
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Keywords

About this book

Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.

Reviews

"...many will find it extremely useful to have this book on hand when trying to achieve high levels of pure, soluble recombinant protein; or incorporating an E. coli expression step into the procedures of functional genomics, proteomics and protein engineering." - Microbiology Today

Editors and Affiliations

  • Applied Molecular Evolution, San Diego, USA

    Peter E. Vaillancourt

Bibliographic Information

  • Book Title: E. coli Gene Expression Protocols

  • Editors: Peter E. Vaillancourt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593011

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-1-58829-008-3Published: 22 October 2002

  • Softcover ISBN: 978-1-61737-302-2Published: 29 December 2011

  • eBook ISBN: 978-1-59259-301-9Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 347

  • Number of Illustrations: 129 b/w illustrations

  • Topics: Biochemistry, general, Cell Biology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

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