Overview
- Editors:
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Gérard Ailhaud
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Université de Nice-Sophia Antipolis, Nice, France
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Table of contents (26 protocols)
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- Hans Hauner, Thomas Skurk, Martin Wabitsch
Pages 239-247
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- Marie-Christine Alessi, Jérôme Aubert, Raymond Négrel
Pages 249-267
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- Keith N. Frayn, Simon W. Coppack
Pages 269-279
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- Barbara Cannon, Jan Nedergaard
Pages 295-303
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- Pierre Barbe, Christian Darimont, Perla Saint-Marc, Jean Galitzky
Pages 305-321
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- Martin Bidlingmaier, Matthias Tschoep
Pages 323-330
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Back Matter
Pages 331-334
About this book
Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.
Editors and Affiliations
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Université de Nice-Sophia Antipolis, Nice, France
Gérard Ailhaud