DNA Repair Protocols

Prokaryotic Systems

  • Pat Vaughan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 152)

Table of contents

  1. Front Matter
    Pages i-x
  2. Assays To Detect And Quantitate DNA Repair Activity

    1. Front Matter
      Pages 1-1
    2. Margarita Sandigursky, William A. Franklin
      Pages 39-48
    3. Amanda J. Watson, Geoffrey P. Margison
      Pages 49-61
    4. Claire G. Cupples, Georgina Macintyre
      Pages 63-73
    5. Frank G. Harmon, Stephen C. Kowalczykowski
      Pages 75-89
    6. Susana Gonzalez, James G. Wetmur
      Pages 107-118
  3. Use Of DNA Repair Enzymes As Tools In Molecular Biology

  4. Back Matter
    Pages 201-209

About this book

Introduction

DNA repair has assumed a new importance with the discovery that malfunctioning of the DNA repair pathways in humans can lead to many disease states. In DNA Repair Protocols: Prokaryotic Systems, well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Each readily reproducible protocol is presented by a hands-on expert in sufficient detail to ensure robust experimental results and is supplemented by chapter introductions, as well as notes offering a wealth of interesting and useful information.
Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology.

Editors and affiliations

  • Pat Vaughan
    • 1
  1. 1.HiberGen Ltd. and University CollegeCorkIreland

Bibliographic information

  • DOI https://doi.org/10.1385/1592590683
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-643-7
  • Online ISBN 978-1-59259-068-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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