MHC Protocols

  • Stephen H. Powis
  • Robert W. Vaughan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 210)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Databases

    1. Front Matter
      Pages 1-1
    2. James Robinson, Steven G. E. Marsh
      Pages 3-21
    3. Roger Horton, Stephan Beck
      Pages 23-42
  3. Polymorphism in Classical and Nonclassical Hla Genes

    1. Front Matter
      Pages 43-43
    2. Lori L. Steiner, Priscilla V. Moonsamy, Teodorica L. Bugawan, Ann B. Begovich
      Pages 113-142
    3. J. Rafael, Martha Pérez-Rodríguez, Andrea Pay, Gaby Fisher, Alasdair McWhinnie, J. Alejandro Madrigal
      Pages 173-189
    4. Paul P. J. Dunn, Steven T. Cox, Ann-Margaret Little
      Pages 191-222
    5. Jorge Martinez-Laso, Eduardo Gomez-Casado, Antonio Arnaiz-Villena
      Pages 223-236
    6. Hélène Teisserenc
      Pages 237-246
  4. Polymorphism in Non-Hla Mhc Genes

    1. Front Matter
      Pages 247-247
    2. Stephen H. Powis
      Pages 249-258
    3. Zeng-Bian Zhu, John E. Volanakis
      Pages 259-268
    4. Peter M. Schneider, Gottfried Mauff
      Pages 269-295
    5. Anthony Gerard Wilson
      Pages 297-304
    6. R. W. M. Collins, Henry A. F. Stephens, Robert W. Vaughan
      Pages 305-321
  5. Microsatellites

    1. Front Matter
      Pages 323-323
    2. Mary Carrington
      Pages 325-332
  6. Back Matter
    Pages 333-340

About this book


Many of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease association and play a pivotal role in the control of immune response. In MHC Protocols, leading experts detail readily reproducible methods for identifying these polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP2, C2, C4, and TNF-a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is written by an investigator who has used the technique extensively and includes troubleshooting tips and notes on pitfalls to avoid.
Comprehensive and highly practical, MHC Protocols offers both experienced and novice investigators not only a state-of-the-art overview of this dramatically evolving area of immunology, but also a reliable guide to the identification of MHC gene polymorphisms.

Editors and affiliations

  • Stephen H. Powis
    • 1
  • Robert W. Vaughan
    • 2
  1. 1.Centre for Nephrology Royal Free and University College Medical SchoolUniversity College LondonLondonUK
  2. 2.South Thames Tissue Typing, Division of MedicineGuy’s Campus, King’s CollegeLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-548-5
  • Online ISBN 978-1-59259-291-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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