Abstract
TAP1 and TAP2 (for transporter associated with antigen processing) are two ATP-binding bassette (ABC) transporter genes located within the class II region of the human major histocompatibility complex (MHC) (1). Their protein products form a heterodimer within the membrane of the endoplasmic reticulum (ER), which transports oligopeptides from the cytoplasm into the lumen of the ER. Once within the ER, peptides are bound by MHC class I molecules and then transported to the cell surface where they can be recognized by cytotoxic T cells (2). Because of their role in antigen processing and their location within the class II region of the MHC, these genes have attracted attention as possible disease susceptibility loci (3–8). In the rat, alleles of TAP2 transport different subsets of peptides (9), although this does not appear to be the case in humans.
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References
Van Edert P. M. (1999) Genes regulating MHC class I processing of antigen. Curr. Opin. Immunol. 11, 82–88
Koopman J. O., Hammerling G. J., and Momburg F. (1997) Generation, intracellular transport and loading of peptides associated with MHC class I molecules. Curr. Opin. Immunol. 9, 80–88
Powis S. H., Rosenberg W. M. C., Hall M., et al. (1993) TAP1 and TAP2 polymorphism in coeliac disease. Immunogenetics 38, 345–350.
Wordsworth B. P., Pile K. D., Gibson K., Burnley R. O., Mockridge I., and Powis S. H. (1993) Analysis of the MHC encoded transporters TAP1 and TAP2 in rheumatoid arthritis: linkage with DR4 accounts for the association with a minor TAP2 allele. Tissue Antigens 42, 153–155.
Powis S. H., Cooper M. D., Trowsdale J., Zhu, Z.-B., and Volanakis J. E. (1994) Major histocompatibility haplotyes associated with immunoglobulin A deficiency and common variable immunodeficiency: analysis of the peptide transporter genes TAP1 and TAP2. Tissue Antigens 43, 261–265.
Jackson D. G. and Capra J. D. (1995) TAP2 association with insulin-dependent diabetes mellitus is aecondary to HLA-DQB1. Hum. Immunol. 43, 57–65
Ma L., Penfornis A., Wang X., et al. (1997) Evaluation of TAP1 polymorphisms with insulin dependent diabetes mellitus in Finnish diabetic patients. The Childhood Diabetes in Finland (DiMe) Study Group. Hum. Immunol. 53, 159–166
Takeuchi F., Nakano K., Matsuta K., et al. (1997) Polymorphism of TAP1 and TAP2 in Japanese patients with rheumatoid arthritis. Tissue Antigens 49, 80–282
Powis S. J., Deverson E. V., Coadwell W. J., et al. (1992). Effect of polymorphism of an MHC-linked transporter on the peptides assembled in a class I molecule. Nature 357, 211–215.
Teisserenc H., Besnault L., Briaud I., et al. (1997) TAP, LMP and HLA-DM polymorphism: 12th International Histocompatibility Workshop study, in Genetic Diversity of HLA. Functional and Medical Implications. Volume 1 (Charron D., ed.), EDK, Paris, pp. 159–170.
Powis S. H. and Teisserenc H. (1997) TAP, LMP and HLADM typing protocols, in Genetic Diversity of HLA. Functional and Medical Implications. Volume 1 (Charron D., ed.), EDK, Paris, pp. 226–235.
Powis S. H., Tonks S., Mockridge I., Kelly A., Bodmer J. G., and Trowsdale J. (1993) Alleles and haplotypes of the MHC encoded ABC transporters TAP1 and TAP2. Immunogenetics 37, 373–380.
Newton C. R., Graham A., Heptinstall L. E., et al. (1989) Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17, 2503–2516.
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© 2003 Humana Press Inc.
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Powis, S.H. (2003). Typing Alleles of TAP1 and TAP2. In: Powis, S.H., Vaughan, R.W. (eds) MHC Protocols. Methods in Molecular Biology™, vol 210. Humana Press. https://doi.org/10.1385/1-59259-291-0:249
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DOI: https://doi.org/10.1385/1-59259-291-0:249
Publisher Name: Humana Press
Print ISBN: 978-0-89603-548-5
Online ISBN: 978-1-59259-291-3
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