Overview
- Editors:
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Joe O’Connell
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Department of Medicine, National University of Ireland, Cork, Ireland
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Table of contents (27 protocols)
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In Situ Localization of mRNA Expression
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- Tatjana Crnogorac-Jurcevic, Torsten O. Nielsen, Nick R. Lemoine
Pages 197-204
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- Paul Ryan, Simon Aarons, Michael W. Bennett, Gary Lee, Gerald C. O’Sullivan, Joe O’Connell et al.
Pages 205-211
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Differential mRNA Expression
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Front Matter
Pages 225-225
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- Valentina I. Shustova, Stephen J. Meltzer
Pages 227-236
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- Benno Mann, Christoph Hanski
Pages 237-249
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Genetic Analysis
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Front Matter
Pages 251-251
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- Frank Waldron-Lynch, Claire Adams, Michael G. Molloy, Fergal O’Gara
Pages 253-263
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RT-PCR in Immunology
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Front Matter
Pages 265-265
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- Manae Suzuki Kurokawa, Kusuki Nishioka, Tomohiro Kato
Pages 267-280
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- Nadège Gruel, Beatrix Kotlan, Marie Beuzard, Jean-Luc Teillaud
Pages 281-300
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- Chang Hoon Nam, Sandrine Moutel, Jean-Luc Teillaud
Pages 301-327
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- Jean Marchand, Pierre Carayon
Pages 329-337
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RT-PCR in Anti-Sense Technology
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Front Matter
Pages 339-339
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- Michael C. Yeung, Allan S. Lau
Pages 341-346
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RT-PCR in cDNA Cloning
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Front Matter
Pages 347-347
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Back Matter
Pages 375-378
About this book
Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
Reviews
"...RT-PCR succeeds in its goal to provide the reader with useful applications relevant to the "wider research community." -Clinical Chemistry
"It is clearly written and can be easily understood by the readers. It is recommended for students, geneticists, immunologists and other specialists in biomedicine." -Folia Microbiologica
Editors and Affiliations
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Department of Medicine, National University of Ireland, Cork, Ireland
Joe O’Connell