Overview
- Includes cutting-edge methods and protocols for adenovirus research
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1089)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents (16 protocols)
-
Front Matter
-
Back Matter
Keywords
About this book
Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.
Editors and Affiliations
Bibliographic Information
Book Title: Adenovirus
Book Subtitle: Methods and Protocols
Editors: Miguel Chillón, Assumpció Bosch
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-679-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-678-8Published: 17 October 2013
Softcover ISBN: 978-1-4939-6325-6Published: 27 August 2016
eBook ISBN: 978-1-62703-679-5Published: 16 October 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XI, 247
Number of Illustrations: 22 b/w illustrations, 15 illustrations in colour
Topics: Microbiology, Virology, Laboratory Medicine
Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Pharma