Receptor Binding Techniques

  • Anthony P. Davenport

Part of the Methods in Molecular Biology book series (MIMB, volume 897)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Brinda K. Rana, Philip E. Bourne, Paul A. Insel
    Pages 1-13
  3. Chidochangu P. Mpamhanga, Joanna L. Sharman, Anthony J. Harmar, NC-IUPHAR
    Pages 15-29
  4. Janet J. Maguire, Rhoda E. Kuc, Anthony P. Davenport
    Pages 31-77
  5. Anke Teichmann, Antje Schmidt, Burkhard Wiesner, Alexander Oksche, Ralf Schülein
    Pages 139-169
  6. Daniel L. Bassoni, Qumber Jafri, Sunitha Sastry, Mahesh Mathrubutham, Tom S. Wehrman
    Pages 171-180
  7. Daniel L. Bassoni, William J. Raab, Philip L. Achacoso, Chin Yee Loh, Tom S. Wehrman
    Pages 181-203
  8. Peter Johnström, Joseph L. Bird, Anthony P. Davenport
    Pages 205-220
  9. Peter Johnström, Tim D. Fryer, Joseph L. Bird, Hugh K. Richards, Anthony P. Davenport
    Pages 221-237
  10. Alessandra P. Princivalle, Rachel M. C. Parker, Terri J. Dover, Nicholas M. Barnes
    Pages 261-302
  11. Back Matter
    Pages 303-309

About this book

Introduction

A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand  to undergo a conformational change, leading to a physiological response or change in cell function.  A ligand can be an endogenous neurotransmitter, hormone, paracrine/autocrine factor, or a synthetic drug that may function as an agonist or antagonist. The third edition of  Receptor Binding Techniques expands upon the methods and techniques used for studying receptors in silico, in vitro and in vivo. Comprehensive chapters describe how to use online resources for experimental research such as prediction of receptor-ligand interactions and mine the IUPHAR receptor database. Classical techniques of radioligand binding, quantitative autoradiography and their analyses are complemented by the use of  immunocytochemistry  for the cellular localization of receptor protein and hybridization to detect receptor mRNA.  Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and  green fluorescent protein to study  trafficking. Non-radioactive, chemiluminescent cAMP and arrestin assays facilitate the identification of novel ‘biased agonists’. Detailed methods are provided for in vivo imaging of receptors using positron emission tomography (PET).

Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Binding Techniques, Third Edition, aids scientists in continuing to study receptor binding.

Keywords

G-protein-coupled receptors Immunocytochemistry PET scanners Radioligand binding anatomical distribution image analysis ligand-receptor binding mRNA orphan receptors phosphor screens quantitative autoradiography

Editors and affiliations

  • Anthony P. Davenport
    • 1
  1. 1.Addenbrooke's Hospital, Box 110, Clinical Pharmacology UnitUniversity of CambridgeCambridgeUnited Kingdom

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-61779-909-9
  • Copyright Information Springer Science+Business Media, LLC 2012
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-61779-908-2
  • Online ISBN 978-1-61779-909-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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