2D PAGE: Sample Preparation and Fractionation

  • Anton Posch

Part of the Methods in Molecular Biology™ book series (MIMB, volume 424)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sample Preparation Basics

  3. Protein Labeling Techniques

    1. Front Matter
      Pages 71-71
    2. Reiner Westermeier, Burghardt Scheibe
      Pages 73-85
    3. Pier Giorgio Righetti, Roberto Sebastiano, Attilio Citterio
      Pages 87-99
    4. s Gruhler, Irina Kratchmarova
      Pages 101-111
    5. Josef Kellermann
      Pages 113-123
    6. Hélian Boucherie, Aurélie Massoni, Christelle Monribot-Espagne
      Pages 125-135
  4. Fractionation of Proteins by Chemical Reagents and Chromatography

About this book

Introduction

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher.

 

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Keywords

Amino acid Escherichia coli Lipid Organelle Polyacrylamid-Gelelektrophorese Proteomics biochemistry biomedicine enzymes glycoprotein membrane proteins polymer protein protein complexes proteins

Editors and affiliations

  • Anton Posch
    • 1
  1. 1.Bio-Rad Laboratories GmbHGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-60327-064-9
  • Copyright Information Humana Press 2008
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-722-8
  • Online ISBN 978-1-60327-064-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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