Summary
Enzymatic methods provide a convenient alternative for overcoming technical disadvantages of mechanical disruption. Protocols for protein extraction from bacteria and Saccharomyces cerevisiae using lytic enzymes are presented in this chapter. Adaptation of the yeast protocol to a microtiter plate format makes this protocol amenable for proteomic applications and high-throughput screening of libraries expressing genetic variants in yeast. This methodology can also be applied to bacteria.
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Acknowledgment
The authors would like to thank the CONICYT (Project 1030797) and the Millennium Scientific Initiative (Millennium Institutes) (ICM –P99-031) for financial support.
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Salazar, O. (2008). Bacteria and Yeast Cell Disruption Using Lytic Enzymes. In: Posch, A. (eds) 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular Biology™, vol 424. Humana Press. https://doi.org/10.1007/978-1-60327-064-9_2
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DOI: https://doi.org/10.1007/978-1-60327-064-9_2
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