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  • © 2020

Transgenic Mouse

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2066)

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9837-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. The History of Transgenesis

    • Thomas L. Saunders
    Pages 1-26
  3. Pronuclear Microinjection of One-Cell Embryos

    • Melissa A. Larson
    Pages 27-33
  4. In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos

    • Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian
    Pages 47-57
  5. Targeted Mutations in the Mouse via Embryonic Stem Cells

    • Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, Lauryl M. J. Nutter
    Pages 59-82
  6. Embryo Transfer Surgery

    • Melissa A. Larson
    Pages 101-106
  7. Nuclear Transfer and Cloning

    • Ling Liu
    Pages 113-124
  8. Sperm-Mediated Genetic Modifications

    • Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni
    Pages 125-132
  9. Genotyping Genetically Modified (GM) Mice

    • Neeraj K. Aryal, Jan Parker-Thornburg
    Pages 133-148
  10. Nomenclature: Naming Your Gene-Modified Mouse

    • Alicia Valenzuela
    Pages 149-162
  11. Breeding Strategies for Genetically Modified Mice

    • Jan Parker-Thornburg
    Pages 163-169
  12. Cryobanking and Recovery of Genetically Modified Mice

    • Toru Takeo, Naomi Nakagata
    Pages 195-209
  13. Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques

    • Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata
    Pages 211-216
  14. Back Matter

    Pages 235-236

About this book

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. 

Keywords

  • pronuclear injection
  • embryonic stem cells
  • Enzymatic modifier systems
  • Spermatogonial
  • Nuclear transfer

Editors and Affiliations

  • Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA, Kansas City, USA

    Melissa A. Larson

Bibliographic Information

  • Book Title: Transgenic Mouse

  • Book Subtitle: Methods and Protocols

  • Editors: Melissa A. Larson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9837-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9836-4

  • Softcover ISBN: 978-1-4939-9839-5

  • eBook ISBN: 978-1-4939-9837-1

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 236

  • Number of Illustrations: 18 b/w illustrations, 27 illustrations in colour

  • Topics: Medical Genetics, Experimental Organisms, Stem Cell Biology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-9837-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 149.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)