Abstract
Embryo transfer surgery is an essential step in the process of generating gene-modified mice, regardless of whether the embryos were modified by DNA, RNA CRISPR components, or embryonic stem cells, or whether they were microinjected or electroporated. Transfer is also a necessary step for assisted reproductive techniques such as rederivation and reanimation by in vitro fertilization. The manipulated embryos must be returned to the reproductive tract of a pseudopregnant recipient female mouse, wherein the transplanted embryos develop to term. Embryos may be transferred to either the oviduct or uterine horn, depending upon the developmental status of the embryos and the stage of the recipient. This chapter will describe the process of transferring embryos surgically to a recipient female.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gunnet JW, Freeman ME (1984) Hypothalamic regulation of mating-induced prolactin release. Effect of electrical stimulation of the medial preoptic area in conscious female rats. Neuroendo 38:12–16
Osada T, Watanabe G, Sakaki Y, Takeuchi T (2001) Puromycin-sensitive aminopeptidase is essential for the maternal recognition of pregnancy in mice. Mol Endocrinol 15(6):882–893
McLaren A, Michie D (1956) Studies on the transfer of fertilized mouse eggs to uterine foster mothers 1. Factors affecting the implantation and survival of native and transferred eggs. J Exp Biol 33:394–416
Byers SL, Wiles MV, Dunn SL, Taft RA (2012) Mouse estrous cycle identification tool and images. PLoS One 7(4):e35538. PMID: 22514749
Zeller W, Meier G, Burki K, Panoussis B (1998) Adverse effects of tribromoethanol as used in the production of transgenic mice. Lab Anim 32(4):407–413
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Larson, M.A. (2020). Embryo Transfer Surgery. In: Larson, M. (eds) Transgenic Mouse. Methods in Molecular Biology, vol 2066. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9837-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9837-1_8
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9836-4
Online ISBN: 978-1-4939-9837-1
eBook Packages: Springer Protocols