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Functional Disulphide Bonds

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Philip Hogg

    1. The Centenary Institute, NHMRC Clinical Trials Centre, Sydney Medical School, University of Sydney, Sydney, Australia

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1967)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Classification of Protein Disulphide Bonds

    • Aster E. Pijning, Philip Hogg
    Pages 1-8

  3. Assessing the Evolutionary Conservation of Protein Disulphide Bonds

    • Jason W. H. Wong
    Pages 9-19

  4. A Proteomics Workflow for the Identification of Labile Disulphide Bonds at the Cell Surface

    • Clive Metcalfe
    Pages 21-43

  5. Quantification of the Redox State of Protein Disulphide Bonds

    • Joyce Chiu
    Pages 45-63

  6. Determining the Redox Potential of a Protein Disulphide Bond

    • Kristina M. Cook
    Pages 65-86

  7. Studying Functional Disulphide Bonds by Computer Simulations

    • Frauke Gräter, Wenjin Li
    Pages 87-113

  8. Dynamic Force Spectroscopy Analysis on the Redox States of Protein Disulphide Bonds

    • Lining Ju
    Pages 115-131

  9. Assays of Thiol Isomerase Enzymatic Activity

    • Roelof H. Bekendam, Robert Flaumenhaft
    Pages 133-148

  10. Functional Assays of Thiol Isomerase ERp5

    • Alexander Dupuy, Freda Passam
    Pages 149-163

  11. Identification of PDI Substrates by Mechanism-Based Kinetic Trapping

    • Oskar Eriksson, Jack Stopa, Bruce Furie
    Pages 165-182

  12. Site-Specific Proteomic Mapping of Modified Cysteine Residues

    • Neal S. Gould
    Pages 183-195

  13. Quantitation of Glutathione, Glutathione Disulphide, and Protein-Glutathione Mixed Disulphides by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

    • Michael R. Bukowski, Matthew J. Picklo Sr.
    Pages 197-210

  14. Interrogation of Functional Mitochondrial Cysteine Residues by Quantitative Mass Spectrometry

    • Daniel W. Bak, Eranthie Weerapana
    Pages 211-227

  15. Oxidative Protein Folding Using trans-3,4-Dihydroxyselenolane Oxide

    • Kenta Arai, Michio Iwaoka
    Pages 229-244

  16. Methodology for Identification of Cysteine-Reactive Covalent Inhibitors

    • Stefan G. Kathman, Alexander V. Statsyuk
    Pages 245-262

  17. In Vivo Measurement of Redox-Regulated TG2 Activity

    • Arek V. Melkonian, Nielson Weng, Brad A. Palanski, Chaitan Khosla
    Pages 263-274

  18. Quantitation of Total and Free Thiol β2-Glycoprotein I Levels for Diagnostic and Prognostic Purposes in the Antiphospholipid Syndrome

    • M. Qi, James C. Weaver, S. A. Krilis, B. Giannakopoulos
    Pages 275-283

  19. Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia

    • M. Qi, James C. Weaver, S. Rahgozar, B. Giannakopoulos, S. A. Krilis
    Pages 285-293

  20. Preparation of a Dithiol-Reactive Probe for PET Imaging of Cell Death

    • Ivan Ho Shon, Michael P. Gotsbacher, Jennifer Guille, Divesh Kumar, Rachel Codd, Philip Hogg
    Pages 295-304
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Keywords

About this book

This book describes the current techniques used to study functional disulphides and to exploit them for therapeutic outcomes. Beginning with how disulphide bonds are classified and how to study their evolution, the volume then continues with protein chemical, molecular dynamics, and force spectroscopy techniques used to identify functional disulphides and/or study their behavior, then focuses on techniques employed in the study of the factors that cleave and/or form disulphide bonds. Methods to study how particular functional disulphides are assayed in mouse and human tissues are likewise examined, amongst many other subjects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, FunctionalDisulphide Bonds: Methods and Protocols serves as a vital guide to this evolving area of study.

Editors and Affiliations

  • The Centenary Institute, NHMRC Clinical Trials Centre, Sydney Medical School, University of Sydney, Sydney, Australia

    Philip Hogg

Bibliographic Information

  • Book Title: Functional Disulphide Bonds

  • Book Subtitle: Methods and Protocols

  • Editors: Philip Hogg

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9187-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9186-0Published: 09 May 2019

  • eBook ISBN: 978-1-4939-9187-7Published: 08 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 327

  • Number of Illustrations: 55 b/w illustrations, 69 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

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