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Protein Terminal Profiling

Methods and Protocols

  • Oliver Schilling

Part of the Methods in Molecular Biology book series (MIMB, volume 1574)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Håvard Foyn, Paul R. Thompson, Thomas Arnesen
    Pages 9-15
  3. Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, Pitter F. Huesgen
    Pages 35-50
  4. An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert et al.
    Pages 51-76
  5. Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, Christine Schaeffer-Reiss
    Pages 77-90
  6. Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, Petra Van Damme
    Pages 115-133
  7. Joanna Tucher, Andreas Tholey
    Pages 171-182
  8. Matej Vizovišek, Robert Vidmar, Marko Fonović
    Pages 183-195
  9. Mercedes Arguello Casteleiro, Robert Stevens, Julie Klein
    Pages 205-213
  10. Anita Chalasani$, Kyungmin Ji$, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin et al.
    Pages 215-225
  11. Angelina Hahlbrock, Dorothée Gößwein, Roland H. Stauber
    Pages 227-241
  12. Catharina Conrad, Miles A. Miller, Jörg W. Bartsch, Uwe Schlomann, Douglas A. Lauffenburger
    Pages 243-253
  13. Tim Van Kersavond, Minh T. N. Nguyen, Steven H. L. Verhelst
    Pages 255-266
  14. Back Matter
    Pages 267-268

About this book

Introduction

This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.

Keywords

N-terminal profiling ChaFRADIC COFRADIC N-CLAP PTAG protease specificity profiling N-terminal acetylation protein N- and C-termini

Editors and affiliations

  • Oliver Schilling
    • 1
  1. 1.Institute of Molecular Medicine and Cell ResearchUniversity FreiburgFreiburgGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6850-3
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6849-7
  • Online ISBN 978-1-4939-6850-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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