Heterologous Expression of Membrane Proteins

Methods and Protocols

  • Isabelle Mus-Veteau

Part of the Methods in Molecular Biology book series (MIMB, volume 1432)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, Frank Bernhard
    Pages 1-21
  3. Stephanie P. Cartwright, Lina Mikaliunaite, Roslyn M. Bill
    Pages 23-35
  4. Federica Angius, Oana Ilioaia, Marc Uzan, Bruno Miroux
    Pages 37-52
  5. Kiavash Mirzadeh, Stephen Toddo, Morten H. H. Nørholm, Daniel O. Daley
    Pages 53-61
  6. Juni Andréll, Patricia C. Edwards, Fan Zhang, Maria Daly, Christopher G. Tate
    Pages 63-78
  7. Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, François André et al.
    Pages 79-101
  8. Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, Soraia Attie Calil Jorge
    Pages 103-118
  9. Lucie Hartmann, Valérie Kugler, Renaud Wagner
    Pages 143-162
  10. Rebba C. Boswell-Casteel, Jennifer M. Johnson, Robert M. Stroud, Franklin A. Hays
    Pages 163-186
  11. Ravi C. Kalathur, Marinela Panganiban, Renato Bruni
    Pages 187-202
  12. Sarika Chaudhary, Sukanya Saha, Sobrahani Thamminana, Robert M. Stroud
    Pages 203-221
  13. Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zöhre Ucurum, Marc J. Bergeron et al.
    Pages 223-242
  14. Katia Duquesne, Valérie Prima, James N. Sturgis
    Pages 243-260
  15. Alice J. Rothnie
    Pages 261-267

About this book

Introduction

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

Keywords

Integral membrane proteins Recombinant IMPs G protein-coupled receptors GPCRs Purification Pharmaceuticals Drug targets

Editors and affiliations

  • Isabelle Mus-Veteau
    • 1
  1. 1.Université Côte d’Azur CNRS, IPMCValbonneFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3637-3
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3635-9
  • Online ISBN 978-1-4939-3637-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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