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RNAi Strategies for Pest Management

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Luis María Vaschetto

    1. Oncativo, Argentina

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2360)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Genome-Wide Identification and Functional Characterization of Noncoding RNAs (ncRNAs) Differentially Expressed During Insect Development

    • Yue Wang, Minsheng You, Weiyi He
    Pages 1-8

  3. Identification and Functional Characterization of Argonaute (Ago) Proteins in Insect Genomes

    • Bernard Moussian, Nicolas Casadei
    Pages 9-17

  4. Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi

    • Takashi Kiuchi, Susumu Katsuma
    Pages 19-31

  5. Guiding RNAi Design Through Characterization of Endogenous Small RNA Pathways

    • Jacob O. Peter, Yulica Santos-Ortega, Alex Flynt
    Pages 33-47

  6. Silencing of Molecular Targets with Relevance to Insecticide Resistance in Colorado Potato Beetle Using dsRNA

    • Raed Bouafoura, Mariem Ben Youssef, Pier Jr Morin
    Pages 49-58

  7. Visualization of RNA Transcripts in Western Corn Rootworm (Diabrotica virgifera virgifera) and Plants by In Situ Hybridization

    • Joseph P. Steimel, Xu Hu
    Pages 59-74

  8. In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control

    • Weilin Zhang
    Pages 75-83

  9. RNAi Feeding Bioassay: A Protocol for dsRNA Screening Against Asian Citrus Psyllid and Related Hemipteran Insects

    • Jonatha dos Santos Silva, Layanna Rebouças de Santana Cerqueira, Wayne Bextine Hunter, Eduardo Chumbinho de Andrade
    Pages 85-90

  10. A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte)

    • Xu Hu, Adane Kassa
    Pages 91-103

  11. Rapid Screening of Myzus persicae (Green Peach Aphid) RNAi Targets Using Tobacco Rattle Virus

    • Honglin Feng, Georg Jander
    Pages 105-117

  12. Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae

    • Sadia Iqbal, John Fosu-Nyarko, Frances Brigg, Michael G. K. Jones
    Pages 119-138

  13. Inhibition of Rhopalosiphum maidis (Corn Leaf Aphid) Growth on Maize by Virus-Induced Gene Silencing with Sugarcane Mosaic Virus

    • Seung Ho Chung, Georg Jander
    Pages 139-153

  14. RT-qPCR for Detection and Expression Monitoring of Core Genes Involved in the RNA Interference Pathways of Western Corn Rootworm (Diabrotica virgifera virgifera)

    • Courtney A. Davis-Vogel
    Pages 155-174

  15. Examination of the Suitability of Attractive Target Genes for RNAi-Based Pest Control

    • Weilin Zhang
    Pages 175-185

  16. Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae

    • John Fosu-Nyarko, Sadia Iqbal, Frances Brigg, Michael G. K. Jones
    Pages 187-208

  17. Plant-Mediated RNA Interference Expressing dsRNA in Cytoplasm for RNAi-Based Pest Control

    • Weilin Zhang
    Pages 209-216

  18. RNAi Expression in Cotton for Control of Herbivorous Insects

    • Ricardo Salvador, Laura Maskin, José Niz, Mariana Turica, Analía Pedarros, Esteban Hopp et al.
    Pages 217-233

  19. Transplastomic Tomato Plants Expressing Insect-Specific Double-Stranded RNAs: A Protocol Based on Biolistic Transformation

    • Emine Kaplanoglu, Igor Kolotilin, Rima Menassa, Cam Donly
    Pages 235-252Open Access

  20. Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri

    • Yulica Santos-Ortega, Alex Flynt
    Pages 253-277
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Keywords

About this book

This volume explores the latest techniques used for sustainable development of eco-friendly pest management systems. Chapters in this book look at the design of novel RNAi-mediated pest control strategies and related issues. Some of the topics covered in this book are identification and characterization of differentially expressed non-coding RNAs in insect genomes; functional small RNA design; analysis of endogenous RNAi pathways; RNAi screening; plant models of RNAi-mediated pest control; and dsRNA delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and comprehensive, RNAi Strategies for Pest Management: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. 


Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.



Editors and Affiliations

  • Oncativo, Argentina

    Luis María Vaschetto

Bibliographic Information

  • Book Title: RNAi Strategies for Pest Management

  • Book Subtitle: Methods and Protocols

  • Editors: Luis María Vaschetto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1633-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1632-1Published: 09 September 2021

  • Softcover ISBN: 978-1-0716-1635-2Published: 10 September 2022

  • eBook ISBN: 978-1-0716-1633-8Published: 08 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 369

  • Number of Illustrations: 14 b/w illustrations, 49 illustrations in colour

  • Topics: Genetics and Genomics, Agriculture

  • Industry Sectors: Biotechnology

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