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A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte)

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RNAi Strategies for Pest Management

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2360))

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Abstract

Identification of active target genes in bioassay screening is the first important step for application of RNA interference (RNAi) for pest control. Here, we describe the methodology for performing high-throughput RNAi target screening against important agriculture pest, Western corn rootworm in 96-well microplate. Two approaches are presented to identify active targets from random-cDNA library or testing a certain group of specific targets via in silico sequence analysis. Methods of PCR primer design, DNA template preparation, and dsRNA production described here can be applied for other pests.

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Acknowledgments

We thank Nina M. Richtman and Bliss Kernodle for DNA and RNA preparations and Lisa Procyk for insect bioassay support.

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Authors and Affiliations

  1. Corteva AgriScience, Johnston, IA, USA

    Xu Hu & Adane Kassa

Authors
  1. Xu Hu
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  2. Adane Kassa
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Corresponding author

Correspondence to Xu Hu .

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Editors and Affiliations

  1. Oncativo, Argentina

    Luis María Vaschetto

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Hu, X., Kassa, A. (2022). A Random-Screening Approach to Identify RNAi Targets for the Control of Western Corn Rootworm (Diabrotica. virgifera virgifera Le Conte). In: Vaschetto, L.M. (eds) RNAi Strategies for Pest Management. Methods in Molecular Biology, vol 2360. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1633-8_9

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  • DOI: https://doi.org/10.1007/978-1-0716-1633-8_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1632-1

  • Online ISBN: 978-1-0716-1633-8

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