Dynamic Analysis of QTLs for Green Leaf Area Duration and Green Leaf Number of Main Stem in Wheat

Abstract

Senescence in a wheat (Triticum aestivum L.) leaf is a programmed degeneration process leading to death. During this process, green leaf area duration (GLAD) and green leaf number of main stem (GLNMS) are gradually reduced. In this study, the two traits of Hanxuan10/Lumai14 DH population at different development stages after anthesis were evaluated under rainfed and irrigated conditions, and QTLs were detected. GLAD and GLNMS of two parents and DH population under rainfed condition were less than those under irrigated condition, and close correlations (P < 0 05) were found between GLAD and GLNMS after 25 DAA under both water conditions. GLAD and GLNMS were co-controlled by major and minor genes. QTLs for GLAD were stably expressed at different development stages after anthesis under both water conditions, such as QGlad22-1B-1, QGlad25-1B-1, QGlad28-1B-2 detected under irrigated condition and QGlad25-1B-3, QGlad28-1B-4 mapped under rainfed condition were located at a 20.7 cM marker interval of Xgwm273-EST122 on 1B chromosome. But QTLs for GLNMS were inducibly and specifically expressed at specific developmental stages after anthesis under both water conditions. The findings provide dynamic genetic information related to wheat senescence.

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References

  1. Bänziger, M., Edmeades, G.O., Lafitte, H.R. 1999. Selection for drought tolerance increases maize yields across a range of nitrogen levels. Crop Sci. 39:1035–1040.

    Article  Google Scholar 

  2. Bekavac, G. 1998. Path analysis of stay-green trait in maize. Cereal Res. Commun. 26:161–167.

    Google Scholar 

  3. Buchanan-Wollaston, V. 1997. The molecular biology of leaf senescence. J. Exp. Bot. 48:181–199.

    Article  Google Scholar 

  4. Fotovat, R., Valizadeh, M., Toorchi, M. 2007. Association between water-use efficiency components and total chlorophyll content (SPAD) in wheat (Triticum aestivum L.) under well-watered and drought stress conditions. Int. J. Food Agric. and Enviorn. 5:225–227.

    CAS  Google Scholar 

  5. Fracheboud, Y., Ribaut, J.M., Vargas, M., Mesamer, R. 2002. Identification of quantitative trait loci for cold tolerance of photosynthesis in maize (Zea mays L.). J. Exp. Bot. 53:1967–1977.

    CAS  Article  Google Scholar 

  6. Hafsi, M., Mechmeche, W., Bouamama, L., Djekoune, A. 2000. Flag leaf senescence as evaluated by numerical image analysis and its relationship with yield under drought in durum wheat. J. Agron. Crop Sci. 185:275–280.

    Article  Google Scholar 

  7. Hervé, D., Francoise, F., Ericka, F.B., Nadia, L. 2001. QTL analysis of photosynthesis and water status traits in sunflower (Helianthus annuus L.) under greenhouse condition. J. Exp. Bot. 52:1857–1864.

    Article  Google Scholar 

  8. Hu, S., Mei, H., Zou, G., Liu, H. 2006. Analysis of quantitative trait loci for chlorophyll content in rice leaves under drought stress. J. Plant Ecol. 30:479–486.

    CAS  Article  Google Scholar 

  9. Jing, R.L., Chang, X.P., Jia, J.Z., Hu, R.H. 1999. Establishing wheat doubled haploid population for genetic mapping by anther culture. Biotechnol. 9:4–8.

    Google Scholar 

  10. Joshi, A.K., Kumari, M., Singh, V.P., Reddy, C.M. 2007. Stay green trait: variation, inheritance and its association with spot blotch resistance in spring wheat. Euphytica 153:59–71.

    Article  Google Scholar 

  11. Kang, S., Zhang, J. 2004. Controlled alternate partial root-zone irrigation, its physiological consequences and impact on water use efficiency. J. Exp. Bot. 55:1–10.

    Google Scholar 

  12. Kumar, U., Joshi, A.K., Kumari, M., Paliwal, R. 2010. Identification of QTLs for stay green trait in wheat (Triticum aestivum L.) in the ‘Chirya 3’×’Sonalika’ population. Euphytica 174:437–445.

    Article  Google Scholar 

  13. Mariana, L., Checovich, Andrea Galatro, Jorge, I. Moriconi. 2016. The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity. Plant Physiol. Bioch. 104:257–265.

    Article  Google Scholar 

  14. Munné-Bosch, S., Alegre, L. 2000. Changes in carotenoids, tocopherols and diterpenes during drought and recovery and the biological significance of chlorophyll loss in Rosmarinus officinalis plants. Planta 210:925–931.

    Article  Google Scholar 

  15. Nooden, L.D., Guiamet, J.J., John, I. 1997. Senescence mechanisms. Physiol. Plant. 101:746–753.

    CAS  Article  Google Scholar 

  16. Pierce, R.O., Knowles, P.F., Phillips, D.A. 1984. Inheritance of delayed leaf senescence in soybean. Crop Sci. 24:515–518.

    Article  Google Scholar 

  17. Rosenow, D.T., Clark, L.E. 1981. Drought tolerance in sorghum. In Proceedings of the 36th Annual Corn and Sorghum Research Conference, Held at Chicago (eds) H.D. Loden, D. Wilkinson), pp. 18–30. Washington, DC: American Seed Trade Association.

  18. Rosenow, D.T., Quisenberry, J.E., Wendt, C.W., Clark, L.E. 1983. Drought tolerant sorghum and cotton germplasm. Agr. Water Manage. 7:207–222.

    Article  Google Scholar 

  19. Shen, B., Zhuang, J., Zhang, K., Dai, W. 2005. Analysis of interaction between QTL and environment on chlorophyll contents in rice. Scientia Agricultura Sinica 38:1937–1943.

    CAS  Google Scholar 

  20. Spano, G., Fonzo, N.Di., Perrotta, C., Platani, C. 2003. Physiological characterization of ‘stay green’ mutants in durum wheat. J. Exp. Bot. 54:1415–1420.

    CAS  Article  Google Scholar 

  21. Tanksley, S.D., Nelson, J.C. 1996. Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor. Appl. Genet. 92:191–203.

    CAS  Article  Google Scholar 

  22. Thomas, H., Howarth, C.J. 2000. Five ways to stay green. J. Exp. Bot. 51:329–337.

    CAS  Article  Google Scholar 

  23. Thomas, H., Smart, C.M. 1993. Crops that stay green. Ann. Appl. Biol. 123:193–219.

    Article  Google Scholar 

  24. Tuberosa, R., Salvi, S., Sanguineti, M.C., Landi, P. 2002. Mapping QTL regulating morpho-physiological traits and yield: Case studies, shortcomings and perspectives in drought stressed maize. Ann. Bot. 89:941–963.

    CAS  Article  Google Scholar 

  25. Verma, V., Foulkes, M.J., Worland, A.J., Sylvesterbradley. 2004. Mapping quantitative trait loci for flag leaf senescence as a yield determinant in winter wheat under optimal and drought stressed environments. Euphytica 135:255–263.

    CAS  Article  Google Scholar 

  26. Vijayalakshmi, K., Fritz, A.K., Paulsen, G.M., Bai, G. 2010. Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature. Mol. Breed. 26:163–175.

    CAS  Article  Google Scholar 

  27. Walulu, R.S., Rosenow, D.T., Wester, D.B., Nguyen, H.T. 1994. Inheritance of the stay-green trait in sorghum. Crop Sci. 34:970–972.

    Article  Google Scholar 

  28. Wang, D.L., Zhu, J., Li, Z.K., Paterson, A.H. 1999. Mapping QTLs with epistatic effects and QTL×environment interactions by mixed linear model approaches. Theor. Appl. Genet. 99:1255–1264.

    Article  Google Scholar 

  29. Wang, S., Liang, Z., Sun, D., Dong, F. 2015. Quantitative trait loci mapping for traits related to the progression of wheat flag leaf senescence. J. Agric. Sci. 153:1234–1245.

    CAS  Article  Google Scholar 

  30. Wu, X. 2008. Genetic analysis of quantitative trait loci for drought resistance in Wheat. Chinese Academy of Agricultural Sciences. PhD thesis, Beijing, China.

    Google Scholar 

  31. Xin, X.X., Li, M.F., Liu, Y. 2018. Genetic analysis of stay-green of flag leaf and thousand-grain weight in introgression lines of wheat under different water conditions. Agric. Res. Arid. Areas. 36:207–212.

    Google Scholar 

  32. Xu, W., Subudhi, P.K., Crasta, O.R., Rosenow, D.T. 2000. Molecular mapping of QTLs conferring stay-green in grain sorghum. Genome 43:461–469.

    CAS  Article  Google Scholar 

  33. Xue, H. 2010. Studies on the changes of endogenous hormones in stay-green wheat varieties and the relationship between the changes of endogenous hormones and stay-green and drought resistance. Master’s thesis, Northwest Agriculture and Forest Science and Technology University, Shaanxi, China.

    Google Scholar 

  34. Yang, D., Jing, R., Chang, X., Li, W. 2007. Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat. J. Integr. Plant Biol. 49:646–654.

    CAS  Article  Google Scholar 

  35. Zhou, X., Jing, R., Chang, X., Zhang, Z. 2005. QTL mapping for water use efficiency and related traits in wheat seedling. J. Plant Genet. Resour. 6:20–25.

    Google Scholar 

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Correspondence to S. G. Wang or D. Z. Sun.

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Communicated by P.S. Baenziger

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Shi, Y.G., Lian, Y., Shi, H.W. et al. Dynamic Analysis of QTLs for Green Leaf Area Duration and Green Leaf Number of Main Stem in Wheat. CEREAL RESEARCH COMMUNICATIONS 47, 250–263 (2019). https://doi.org/10.1556/0806.47.2019.06

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Keywords

  • double haploid population
  • senescence
  • major gene
  • minor gene
  • correlation