Comparison of Hungarian and Bulgarian Isolates of Maize Dwarf Mosaic Virus
Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen. Maize dwarf mosaic virus (MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.
KeywordsMaize dwarf mosaic virus corn coat protein sequence similarity SCMV subgroup
Unable to display preview. Download preview PDF.
- Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L., Zurcher, E.J. (eds) 1996 onwards. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20 th August 1996. URLGoogle Scholar
- Janson, B.F., Ellett, C.W. 1963. A new corn disease in Ohio. Plant Dis. Rep. 47:1107–1108.Google Scholar
- Kovachevsky, I., Markov, M., Yankulova, M., Trifonov, D., Stoyanov, D., Kacharmazov, V. 1995. Virus and Virus-like Diseases of Crop Plants. PSSA, Sofia, pp. 143–148. (in Bulgarian)Google Scholar
- Markov, M. 1972. Studies on maize mosaic in Bulgaria. I. Identification of the virus. Plant Science 9:171–179. (in Bulgarian with English and Russian summary)Google Scholar
- Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. II. Establishing the host plants of the virus. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 55–67. (in Bulgarian with English and Russian summary)Google Scholar
- Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. III. Virus purification. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 139–145. (in Bulgarian with English and Russian summary)Google Scholar
- Shepherd, R.J. 1965. Properties of a mosaic virus of corn and Johnson grass and its relation to the sugarcane mosaic virus. Phytopathology 55:1250–1256.Google Scholar
- Shukla, D.D., Tosic, M., Jilka, J., Ford, R.E., Toler, R.W., Langham, M.A.C. 1989. Taxonomy of potyviruses infecting maize, sorghum, and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79:223–229.CrossRefGoogle Scholar
- Shukla, D.D., Ward, C.W., Brunt, A.A. 1994. The Potyviridae. CAB International, Wellingford, UK.Google Scholar
- Szirmai, J., Paizs, L-né 1963. Maize mosaic or stripe. Növénytermelés 12:43–50. (in Hungarian, with English summary)Google Scholar
- Williams, L.E., Alexander, L.J. 1965. Maize dwarf mosaic virus, a new corn disease. Phytopathology 58:802–804.Google Scholar