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Cereal Research Communications

, Volume 35, Issue 4, pp 1643–1651 | Cite as

Comparison of Hungarian and Bulgarian Isolates of Maize Dwarf Mosaic Virus

  • I. TóbiásEmail author
  • N. Bakardjieva
  • L. Palkovics
Pathology

Abstract

Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen. Maize dwarf mosaic virus (MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.

Keywords

Maize dwarf mosaic virus corn coat protein sequence similarity SCMV subgroup 

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References

  1. Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L., Zurcher, E.J. (eds) 1996 onwards. Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20 th August 1996. URLGoogle Scholar
  2. Frenkel, M.J., Jilka, J.M., McKern, N.M., Strike, P.M., Clark Jr., J.M., Shukla, D.D., Ward, C.W. 1991. Unexpected sequence diversity in the amino-terminal ends of the coat proteins of strains of sugarcane mosaic virus. Journal of General Virology 72:237–242.CrossRefGoogle Scholar
  3. Janson, B.F., Ellett, C.W. 1963. A new corn disease in Ohio. Plant Dis. Rep. 47:1107–1108.Google Scholar
  4. Jarjees, M.M., Uyemoto, J.K. 1984. Serological relatedness of strains of maize dwarf mosaic and sugarcane mosaic viruses as determined by microprecipitin and enzyme-linked immunosorbent assays. Ann. Appl. Biol. 104:497–501.CrossRefGoogle Scholar
  5. Kong, P., Steinbiss, H.-H. 1998. Complete nucleotide sequence and analysis of the putative polyprotein of maize dwarf mosaic virus genomic RNA (Bulgarian isolate). Archives of Virology 143:1791–1799.CrossRefGoogle Scholar
  6. Kovachevsky, I., Markov, M., Yankulova, M., Trifonov, D., Stoyanov, D., Kacharmazov, V. 1995. Virus and Virus-like Diseases of Crop Plants. PSSA, Sofia, pp. 143–148. (in Bulgarian)Google Scholar
  7. Markov, M. 1972. Studies on maize mosaic in Bulgaria. I. Identification of the virus. Plant Science 9:171–179. (in Bulgarian with English and Russian summary)Google Scholar
  8. Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. II. Establishing the host plants of the virus. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 55–67. (in Bulgarian with English and Russian summary)Google Scholar
  9. Markov, M. 1974. Studies on Maize Mosaic in Bulgaria. III. Virus purification. Plant virus diseases. Publishing House of the Bulgarian Academy of Sciences, Sofia, pp. 139–145. (in Bulgarian with English and Russian summary)Google Scholar
  10. Pirone, T.P., Blanc, S. 1996. Helper-dependent vector transmission of plant viruses. Ann. Rev. Phytopathology 34:227–247.CrossRefGoogle Scholar
  11. Rosenkranz, E. 1987. New hosts and taxonomic analysis of the Mississippi native species tested for reaction to maize dwarf mosaic and sugarcane mosaic viruses. Phytopathology 77:598–607.CrossRefGoogle Scholar
  12. Seifers, D.L., Salomon, R., Marie-Jeanne, V., Alliot, B., Signoret, P., Haber, S., Loboda, A., Ens, W., She, Y.-M., Standing, K.G. 2000. Characterization of a novel potyvirus isolated from maize in Israel. Phytopathology 90:505–513.CrossRefGoogle Scholar
  13. Shepherd, R.J. 1965. Properties of a mosaic virus of corn and Johnson grass and its relation to the sugarcane mosaic virus. Phytopathology 55:1250–1256.Google Scholar
  14. Shukla, D.D., Tosic, M., Jilka, J., Ford, R.E., Toler, R.W., Langham, M.A.C. 1989. Taxonomy of potyviruses infecting maize, sorghum, and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79:223–229.CrossRefGoogle Scholar
  15. Shukla, D.D., Frenkel, M.J., McKern, M.N., Ward, C.W., Jilka, J., Tosic, M., Ford, R.E. 1992. Present status of sugarcane mosaic subgroup of potyviruses. Archives of Virology Suppl. 5:363–373.CrossRefGoogle Scholar
  16. Shukla, D.D., Ward, C.W., Brunt, A.A. 1994. The Potyviridae. CAB International, Wellingford, UK.Google Scholar
  17. Snazelle, T.E., Bancroft, J.B., Ullstrup, A.J. 1971. Purification and serology of maize dwarf mosaic and sugarcane mosaic viruses. Phytopathology 61:1059–1063.CrossRefGoogle Scholar
  18. Szirmai, J., Paizs, L-né 1963. Maize mosaic or stripe. Növénytermelés 12:43–50. (in Hungarian, with English summary)Google Scholar
  19. Tóbiás, I., Palkovics, L. 2004. An unusual feature at the N-terminal end of the coat protein of Maize dwarf mosaic virus isolated in Hungary. Journal of Phytopathology 152:445–447.CrossRefGoogle Scholar
  20. White, J.L., Kaper, J.M. 1989. A simple method for detection of viral satellite RNAs in small tissue samples. Journal of Virological Methods 23:83–94.CrossRefGoogle Scholar
  21. Williams, L.E., Alexander, L.J. 1965. Maize dwarf mosaic virus, a new corn disease. Phytopathology 58:802–804.Google Scholar

Copyright information

© Akadémiai Kiadó, Budapest 2007

Authors and Affiliations

  1. 1.Plant Protection Institute of the Hungarian Academy of SciencesBudapestHungary
  2. 2.Plant Protection InstituteKostinbrodBulgaria
  3. 3.Department of Plant Pathology, Faculty of Horticultural ScienceCorvinus University of BudapestBudapestHungary

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