Modulation of tumor fatty acids, through overexpression or loss of thyroid hormone responsive protein spot 14 is associated with altered growth and metastasis
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Spot14 (S14), encoded by the THRSP gene, regulates de novo fatty acid synthesis in the liver, adipose, and lactating mammary gland. We recently showed that S14 stimulated fatty acid synthase (FASN) activity in vitro, and increased the synthesis of fatty acids in mammary epithelial cells in vivo. Elevated de novo fatty acid synthesis is a distinguishing feature of many solid tumors compared with adjacent normal tissue. This characteristic is thought to be acquired during tumor progression, as rapidly proliferating cells have a heightened requirement for membrane phospholipids. Further, overexpression of FASN is sufficient to stimulate cell proliferation. While many studies have focused on the FASN enzyme in cancer biology, few studies have addressed the roles of proteins that modify FASN activity, such as S14.
Tumor fatty acids were modulated using two mouse models, mouse mammary tumor virus (MMTV)-neu mice overexpressing S14 and MMTV-polyomavirus middle T antigen (PyMT) mice lacking S14, and associations between elevated or impaired fatty acid synthesis on tumor latency, growth, metastasis, and signaling pathways were investigated. We evaluated S14-dependent gene expression profiles in mouse tumors by microarray and used publicly available microarray datasets of human breast tumors.
S14 overexpression in the MMTV-Neu transgenic model is associated with elevated medium-chain fatty acids, increased proliferation and a shorter tumor latency, but reduced tumor metastasis compared to controls. Loss of S14 in the MMTV-PyMT model decreased FASN activity and the synthesis of medium-chain fatty acids but did not alter tumor latency. Impaired fatty acid synthesis was associated with reduced solid tumor cell proliferation, the formation of cystic lesions in some animals, and decreased phosphorylation of Src and protein kinase B (Akt). Analysis of gene expression in these mouse and human tumors revealed a relationship between S14 status and the expression of genes associated with luminal epithelial differentiation.
This study demonstrates a potential role for S14 in regulating mammary tumor growth and fatty acid synthesis in vivo. Furthermore, these results suggest that modulating the amount of medium chain fatty acids, by changing the levels of S14, has the potential to impact malignant mammary tumor phenotypes.
KeywordsMammary Tumor Fatty Acid Synthesis Medium Chain Fatty Acid PyMT Mouse Lactogenic Differentiation
acetyl Co-A carboxylase
Database for Annotation, Visualization and Integrated Discovery
Dulbecco's modified Eagle's medium
fatty acid synthase
gas chromatography-mass spectrometry
NCBI Gene Expression Omnibus
hematoxylin and eosin
Kyoto Encyclopedia of Genes and Genomes
medium chain fatty acids
mouse mammary tumor virus
polyomavirus middle T antigen
thyroid hormone responsive protein Spot 14
Spot14 (S14), the protein product of the thyroid hormone responsive protein spot14 (Thrsp) gene, is consistently associated with regulation of the de novo fatty acid synthesis pathway. S14 was originally discovered as a protein that was induced in the liver of fasted rats stimulated by lipogenic factors . Mice lacking the S14 gene produce milk with 60% lower levels of de novo synthesized fatty acids during lactation, compared to wild-type mice, and are resistant to diet-induced obesity -. We previously characterized gene expression profiles of the mouse mammary gland throughout pregnancy and lactation to identify factors that regulate the sharp increase in fatty acid synthesis observed at parturition . This study of the mammary epithelium revealed that S14 expression was associated with lactogenic differentiation, and its expression mirrored the pattern of fatty acid synthesis enzymes, acetyl CoA carboxylase (ACC), ATP-citrate lyase (ACLY) and fatty acid synthase (FASN) during the transition from pregnancy to lactation. In addition to its role in normal mammary function, S14 was shown to positively regulate de novo fatty acid synthesis and cell proliferation in human breast cancer cells ,. We have recently demonstrated that mammary epithelial cell overexpression of S14 in transgenic mice increases the de novo fatty acids present in milk . Furthermore, we showed that addition of recombinant S14 to the FASN enzyme in vitro reduces the apparent Km, increases the apparent Vmax, and increases the synthesis of medium chain fatty acids (MCFA) relative to FASN alone . These observations provide a mechanistic explanation for the described biological effects of S14, particularly in the mammary epithelium during lactation.
Many solid tumors display increased fatty acid synthesis compared to adjacent normal tissue . This is due in part to increased Fasn expression and presumably, increased activity of the FASN enzyme . While it appears that elevated levels of FASN are acquired during tumor progression, its exact role in tumorigenesis is not clear . Increased tumor de novo fatty acid synthesis is predicted to provide several advantages: First, cancer cells are rapidly dividing and have a heightened requirement for membrane phospholipid precursors. Additionally, certain fatty acids can regulate intracellular signaling pathways either by activating signaling molecules or via post-translational modification of pro-tumorigenic kinases, such as Ras and Src -. Finally, elevated fatty acid synthesis may provide a survival advantage for cancer cells, allowing them to store lipids, which could serve as an energy reserve during the metastatic process. Although the requirement of FASN for breast cancer cell survival and proliferation has been established in vitro and in xenograft models, it is not known whether elevated fatty acid synthesis is sufficient to promote cancer metastasis ,. S14 represents a family of proteins that does not have enzymatic activity, but that modulates the activity of de novo fatty acid synthesis pathway enzymes to alter cellular physiology ,,,.
In human tumors, elevated S14 protein was reported to associate with a poor outcome for patients with invasive breast cancer . Furthermore, reduced breast tumor expression of S14, but not FASN, was accompanied by a lower proliferation index in women treated with conjugated linolenic acid . Despite these studies, little consideration has been given to proteins, like S14, that can enhance FASN activity. Although elevated FASN expression and its activity are features of both the differentiated and the neoplastic mammary epithelium, the timing of FASN activation and its cellular context are critical to both the uses of newly synthesized fatty acids and their biological effects upon mammary cells. Our observation that S14 can stimulate FASN enzymatic activity in vitro led to the hypothesis that overexpression of S14 in the mammary epithelium would support tumorigenesis through its ability to enhance de novo fatty acid biosynthesis. To date, no studies have directly addressed a causal link between S14 activation of de novo fatty acid synthesis and mammary tumorigenesis in vivo. In the present study, we have modulated the levels of de novo synthesized fatty acids in mammary tumors using two transgenic mouse models that either overexpress Thrsp in the mammary gland or lack Thrsp. Overexpression of Thrsp in the mammary glands of MMTV-Neu mice shortened tumor latency and increased tumor proliferation, which was associated with increased levels of MCFA (carbon chain ≤16). Conversely, Thrsp loss from MMTV-PyMT mice resulted in slower tumor growth, reduced FASN activity, and lower levels of MCFA compared to controls. MMTV-PyMT tumors lacking Thrsp also had reduced levels of phosphorylated Src and Akt, suggesting a possible role for MCFA in potentiating signaling pathways in tumor cells. Surprisingly, tumors from MMTV-Neu mice overexpressing Thrsp had a significant increase the expression of genes associated with luminal differentiation, which was concomitant with decreased lung metastasis. Collectively, these data demonstrate a role for S14 in regulating fatty acid synthesis and mammary tumorigenesis in vivo.
The MMTV-Neu line 202 mice  (FVB/N-Tg(MMTVneu)202Mul/J; stock number 002376) and the MMTV-PyMT  (FVB/N-Tg(MMTVPyVT)634Mul/J; stock number 002374) were obtained from The Jackson Laboratory (Bar Harbor, MN, USA). The Thrsp -/- mice , herein referred to as S14 -/-, (C57Bl/6 background) were kindly provided by Cary Mariash (IU Health Hospital, Indianapolis, IN, USA). S14 -/- mice were backcrossed for ten generations to FVB/N mice using a speed congenic approach. All mice were genotyped by PCR amplification of DNA isolated from tail tissue. The MMTV-S14 mice have been described previously . Hemizygous MMTV-Neu males were crossed to hemizygous MMTV-S14 females and the resulting offspring genotyped for the presence of both transgenes. Female offspring carrying the Neu transgene or both the Neu and S14 transgenes were used for tumor studies. Mice were sacrificed when the largest tumor reached 0.5 cm3.
Male MMTV-PyMT hemizygous mice were crossed with female S14−/− mice and the progeny genotyped for the presence of the MMTV-PyMT transgene. The resulting MMTV-PyMT, S14+/− male mice were crossed with S14+/− females. The offspring used for the tumor studies were MMTV-PyMT/S14−/− and MMTV-PyMT/ S14+/+ females. MMTV-PyMT/Spot14+/− mice were not analyzed in this study. For brevity, the MMTV-PyMT mice are referred to as PyMT and the MMTV-PyMT/S14−/− mice are referred to as PyMT/S14−/− in the text. All animals were maintained in the Center for Comparative Medicine and were provided water and standard rodent chow ad libitum. These studies were approved by the University of Colorado Denver Institutional Animal Care and Use Committee.
For immunohistochemistry (IHC), dissected tumors were fixed in 10% neutral buffer formalin (NBF) and processed by the Pathology Shared Resource of the University of Colorado Cancer Center. Five-micrometer sections were processed for standard staining with hematoxylin and eosin. For IHC analysis of Ki67, antigen retrieval was performed in a pressure cooker using Dako Tissue Retrieval Solution, pH 6 (Dako, Carpinteria, CA, USA; S1699). Following incubation with 3% H2O2 (Sigma Aldrich, St. Louis, MO, USA)) in methanol, slides were blocked with Dako Protein Block (Dako, Carpinteria, CA, USA; X0909) and incubated overnight at 4C with rat anti-Ki67 antibody (Dako Carpinteria, CA, USA; M7249) diluted in Dako antibody diluent (Dako, Carpinteria, CA, USA; S0809). The ImmPRESS anti-rat Ig (Vector Labs, Burlingame, CA, USA; MP7444) was used at room temperature for 1 hour and slides were developed using the Dako DAB chromagen kit (Dako, Carpinteria, CA, USA; K3468). Slides were counterstained with Mayer’s hematoxylin. Ki67 staining was quantified using the Ventana Medical Systems (Tucson, AZ, USA) VIAS camera and software. Five to twelve non-overlapping 20x fields in five tissue sections of each group were used to determine the percent of Ki67-positive cells.
For mammary whole mounts, inguinal mammary glands were dissected, fixed in 10% NBF overnight, stained with carmine alum solution, dehydrated, and mounted between glass slides for visualization. A detailed description has been published elsewhere .
Gas chromatography-mass spectrometry (GC-MS) analysis
Tumor fatty acids were isolated and analyzed by GC-MS as previously described .
FASN activity assay
Tumors were pulverized in liquid nitrogen and 30 to 60 mg of tissue was lysed in FASN activity buffer to generate cytosolic lysates (250 mM sucrose, 20 mM HEPES pH 7.6, 2 mM MgCl2, 1 mM DTT, 1 mM EDTA, 50 mM NaF, 5 mM NaVO4, and 3x final concentration of Halt™ Protease Inhibitor cocktail (Thermo Scientific; Waltham, MA, USA)). FASN abundance was quantified for each sample and a volume of cytosolic lysate representing 5 μg of FASN per sample was analyzed for FASN activity as previously described ,.
Tumors were pulverized in liquid nitrogen and protein was extracted from a portion of the tissue by homogenizing in cell lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 1% Trition X-100, 1% DOC, 0.1% SDS, 1 mM DTT, 5 mM sodium orthovanadate) containing protease inhibitor cocktail (10 μg/μl leupeptin, 10 μg/μl aprotinin, 10 μM pepstatin A, 1 mM PMSF (L2023, A1153, P4265, and P7626, respectively; Sigma Aldrich, St. Louis, MO, USA)). The homogenates were centrifuged at 13,900 rpm for 15 minutes at 4°C, and supernatants were collected, snap frozen and stored at −20°C until use. Anti-phospho-Akt Ser473 (#9271), anti-Akt (#9272), anti-phospho-Src Tyr416 (#2101), anti-phospho-PI3K p85 (#4292), anti-PI3K p85 (#4257), anti-ACLY (#4332), anti-ACC (#3676), and anti-Actin (#5125) were obtained from Cell Signaling Technology (Beverly, MA, USA). Anti-PyMT (sc-53481), anti-Neu (sc-284), anti-FASN (sc-20140), anti-β-tubulin (sc-9104), and anti-Krt18 (sc-28264) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-S14 antibody (ab97504) was obtained from Abcam (Cambridge, MA, USA). The monoclonal antibody directed against amino acids 2 to 17 of Src was prepared by the University of Colorado Cancer Center Monoclonal Antibody, Protein Production, and Tissue Culture Shared Resource. Protein bands were visualized using SuperSignal West Pico chemiluminescent substrate (Thermo Scientific, Waltham, MA, USA) and film. ImageJ  was used to quantify protein bands. Alternatively, cytosolic extracts were prepared as described in the FASN Activity Assay section, analyzed by SDS-PAGE and imaged/quantified using the LiCOR Odyssey system as described previously .
Quantitative PCR (qPCR)
Gene expression was analyzed by qPCR using primers and FAM-labeled probes or SYBR green chemistry. Primers and probe sequences or catalog numbers are listed in Additional file 1. Unless otherwise indicated, all genes assayed are mouse. Transcript copy number was estimated using a standard curve generated from a known concentration of amplicon for each reaction. Additional details have been described elsewhere .
Human breast cancer cells were provided by the University of Colorado Cancer Center Protein Production, Monoclonal Antibody and Tissue Culture Shared Resource and used within 6 months. Cell line identity was validated by STR analysis prior to purchase. Cells were grown in DMEM (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Hyclone, Thermo Scientific, Waltham, MA, USA) and 1% antibiotic/anti-mycotic solution (Sigma Aldrich, St. Louis, MO, USA). RNA was isolated using the RNEasy Mini Kit (Qiagen, Valencia, CA, USA) and cDNA was prepared using the Verso cDNA synthesis kit (Thermo Scientific, Waltham, MA, USA).
Human microarray data analysis
Datasets of primary human breast tumors were obtained from the NCBI Gene Expression Omnibus (GEO) or from Oncomine . All data were normalized within datasets. Datasets were individually sorted by S14 expression, and the top and bottom 25% of the samples were categorized as high and low S14-expressing, respectively. Details of each dataset are listed in Additional file 2. Within the high and low S14-expressing groups, those samples for which either estrogen receptor (ER) IHC status (positive or negative) and intrinsic subtype classification (luminal or basal) were available were used to create 2 × 2 contingency tables. Fisher’s exact test was used to determine statistical significance for comparisons of tumor subtypes and ER status.
Tumor microarray analysis
Total RNA was isolated from tumor tissue using Trizol (Life Technologies, Carlsbad, CA, USA). The RNEasy Mini Kit (Qiagen, Valencia, CA, USA) with optional on-column DNAse digestion was used on the final RNA. One hundred micrograms (Neu and Neu/S14 tumors) or 250 micrograms (PyMT and PyMT/S14−/− tumors) of total RNA was prepared for microarray analysis using the Whole Transcript Expression RNA kit (Life Technologies, Carlsbad, CA, USA) and samples were hybridized to mouse 1.1 ST gene arrays (Affymetrix, Santa Clara, CA, USA). Arrays were washed, stained and imaged using the Affymetrix Gene Atlas Microarray System. Raw data were analyzed using Partek Genomic Suite. Select genes were validated by qPCR (see Additional file 3). These studies can be accessed the NCBI GEO database [GSE42708 and GSE55886]. GO enrichment analysis was performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) bioinformatics resource ,. Only genes with fold change of +/− 1.2 and P <0.05 were considered for analyses, and separate DAVID analyses were performed on genes that were either increased or decreased to allow an interpretation of networks that were enriched in either direction. Full gene lists are available upon request. We presented those GO terms that were enriched ≥2-fold with P ≤0.01. Four tumors from PyMT and PyMT, S14−/− mice were analyzed, thirteen tumors from Neu mice, and fourteen tumors from Neu/S14mice.
Unless otherwise stated, the unpaired, two-tailed t-test was performed to determine statistical significance.
Microarray analysis was performed to better characterize the differences between tumors from PyMT and PyMT/S14−/− mice and also between tumors from Neu and Neu/S14 mice. After identifying genes that were significantly increased or decreased between different experimental groups (PyMT versus PyMT/S14−/− or Neu versus Neu/S14), we performed gene ontology (GO) enrichment analyses to identify the biological processes that were impacted by S14 manipulation.
In PyMT tumors, the loss of S14 led to reduced expression of genes associated with glucose metabolism, protein kinase activity, and cell proliferation (Additional file 7). In this model, the only significantly enriched GO term for genes that increased in tumors lacking S14 was the cell cycle (fold enrichment = 2.81; P = 0.002). S14 overexpression in Neu tumors was associated with elevated levels of genes involved in protein translation, positive regulation of growth, and the electron transport chain (Additional file 8). Conversely, tumors overexpressing S14 had decreased levels of genes associated with the development of the heart, skeletal system, and blood vessels (Additional file 9). The genes in these networks appeared to generally represent two categories with regards to the mammary gland and breast cancer. They have been reported to function during early mammary placode formation in the developing embryo (that is, Msx2, Tbx3, Wnt3a, and Fgfr1) or they are involved in breast cancer cell migration, invasion, and proliferation (that is, Barx2, Pdgfrb, Bmp6, and Jak3) -.
When taken together, these data have several implications. First, we observe differential effects of S14 loss or overexpression on metabolic pathways. Loss of S14 from PyMT tumors is linked to decreased glycolytic gene expression, while S14 overexpression in Neu tumors is associated with increased expression of electron transport chain genes. Because PyMT-driven tumors grow so much more rapidly than those in Neu mice, we postulate that tumors from PyMT mice are highly dependent on glycolysis for ATP production and to generate building blocks for cell division. The observation that S14 loss from PyMT tumors leads to a decrease in cell proliferation is not surprising given that these tumors also have a decrease in glycolytic genes, as cell cycle progression and metabolism are tightly intertwined. Conversely, because S14 overexpression in Neu tumors is associated with elevated proliferation, those tumors are also likely more metabolically active, and this is reflected in greater expression of mitochondrial metabolism genes. Overall, the gene expression data are consistent with the differences in tumor growth rates and cell proliferation resulting from S14 loss or overexpression in both mouse models.
In addition to those related to metabolism, we observed differences in genes known to be associated with functional differentiation of the normal mammary gland in Neu/S14 tumors (Figure 5H). One of the most significantly elevated genes in Neu/S14 tumors was ETS-related transcription factor Elf5, which regulates alveolar differentiation during pregnancy in the mouse mammary gland . The expression of milk protein genes Csn2, Csn1s1, and Csn1s2a was also elevated in Neu/S14 versus Neu tumors (Figure 5H). Recently, Elf5 was shown to repress an EMT-like phenotype during normal mammary gland development and to prevent metastasis in two mouse models of mammary tumorigenesis . When we consider the overall gene expression profile of tumors from Neu/S14 mice, we postulate that overexpression of S14 is associated with reduced levels of genes associated with early mammary stem cells and with breast cancer invasion and metastasis, as well as the elevated expression of genes involved with alveolar epithelial differentiation. Altogether, this gene expression profile is consistent with reduced tumor metastasis, associated with S14 overexpression, observed in this model (Figure 5C). Expression of Csn genes was also evaluated in the tumors from PyMT and PyMT/S14−/− mice (Figure 5I). There was no significant difference in the expression of Csn2 and Csn1s2a between PyMT tumors and the solid tumors from PyMT/S14−/− mice (Figure 5I); however, the cystic tumors that formed in PyMT/S14−/− mice had lower expression of Csn2 and Csn1s2a than those from PyMT mice or than the solid tumors from PyMT/S14−/− mice, indicating an altered differentiation profile in a subset of PyMT/S14−/− tumors. Krt18 expression was not different between any tumor groups (Figure 5I).
In this study, the role of S14 in the regulation of mammary tumor growth was investigated in vivo using mouse models in which S14 levels were genetically modified. We and others have previously shown that S14 regulates de novo fatty acid synthesis in the normal mammary epithelium during lactation ,; and others have shown that S14 regulates de novo fatty acid synthesis in breast cancer cell lines ,,. We recently showed that S14 altered FASN enzyme catalysis in vitro by increasing the apparent Vmax and decreasing the apparent Km, which resulted in a S14-mediated enhancement of de novo fatty acid synthesis . Furthermore, we showed that S14 transgenic mice produced significantly greater quantities of MCFA in milk during lactation . Here, we have demonstrated a role for S14 in modulating the biology of mammary tumors, potentially through modulation of de novo fatty acid synthesis. Overexpression of S14 in Neu-induced mammary tumors shortened tumor latency and increased tumor cell proliferation (Figure 3); and these differences in tumor growth were associated with elevated tumor levels of MCFA relative to Neu controls (Figure 4). Conversely, the absence of S14 from PyMT-induced mammary tumors was associated with slower tumor growth and decreased proliferation (Figures 1 and 2), which was coincident with decreased FASN activity and lower levels of MCFA in tumors lacking S14 compared to controls (Figure 4). Combined, both tumor models suggest that S14 potentiates de novo fatty acid synthesis in vivo, which could result in altered mammary tumor growth.
Analysis of human breast tumors identified an association between high S14 expression and features of luminal differentiation (Figure 6). Specifically, tumors with high S14 expression were significantly more likely to represent the luminal versus basal subtype, and were more likely to be ER+ than ER−. Consistent with the observation from human breast tumors, tumors from Neu/S14 mice also had increased expression of many genes associated with luminal differentiation of mammary epithelial cells, compared to those from Neu mice. Contrary to our hypothesis, overexpression of S14 in Neu-induced tumors resulted in fewer lung metastases compared to controls. These results suggest that tumors from Neu/S14 mice may be less likely to metastasize, in part, because the cells are well differentiated. We have found that overexpression of S14 in normal mouse mammary epithelial cells did not increase the expression of genes associated with lactogenic differentiation (data not shown). Moreover, MMTV-S14 transgenic mice do not display features of lactogenic differentiation in either the gene expression profiles or gland morphology . The frequency of mammary tumor metastasis was also quantified in the PyMT mice with or without S14. While loss of S14 expression was associated with reduced tumor cell proliferation, it did not appear to affect tumor metastasis to the lungs in PyMT mice (Figure 6), suggesting that the effects of S14 on tumor cell proliferation could be independent of processes that govern tumor metastasis.
S14 (reported as Thrsp), one of several genes associated with luminal epithelial differentiation, was found to be downregulated as PyMT-driven mammary tumors progressed from adenomas to carcinomas , indicating that S14 may be expressed in a relatively well-differentiated tumor cell type in the PyMT model. Notably, in the PyMT model, only mice lacking S14 expression produced cystic lesions, while PyMT mice formed solid tumors, and the cystic lesions appearing in PyMT/S14−/− mice had reduced expression of casein genes compared to the solid lesions from either group of mice (Figure 5). This observation is consistent with the observed gene expression profile in tumors from Neu/S14 mice . The cystic lesions that developed in the absence of S14 were reminiscent of those that formed in the same model when the mice were treated with the Src inhibitor SKI606 ; however, Hebbard et al. reported expression of β-Casein in the lumens of the lesions from SKI606-treated mice. Although the lesions that form in PyMT mice treated with the Src inhibitor and those from PyMT/S14−/− mice appear similar in morphology, they likely do not have the same molecular characteristics with regards to cellular differentiation. Cumulatively, these data indicate that S14 itself is not sufficient to promote a differentiation program in mammary tumors; rather, S14 may support the proliferation or survival of a differentiated mammary tumor cell type.
Both Src and Akt rely on cellular lipids for their activity -. Src-family kinases are modified by myristate and palmitate, both of which are MCFA, and myristoylation of c-Src enhances its kinase activity . The lipid-dependent activation of Akt occurs through the binding of its plextrin-homology (PH) domain to phosphatidyl-inositol-trisphosphate (PIP3) present in the plasma membrane ,. The phosphorylation of Src and Akt at their active sites was decreased in PyMT tumors lacking S14 (Figure 7), suggesting that signal transduction through these kinases may be diminished; however, we did not examine the in vivo activities of these enzymes. In addition, levels of PyMT were reduced in tumors lacking S14. Currently, it is unclear how modulating S14 levels or de novo fatty acid synthesis affects the membrane lipid profiles in either PyMT- or Neu-induced mammary tumors. Furthermore, it is not clear how changes in tumor cell lipid profiles impact the post-translational modification or activation of critical signaling molecules, or how changes in membrane phospholipids affect the stability of the PyMT oncogene. Our results suggest that changing the tumor fatty acid profile may be a way to alter pro-tumorigenic signaling pathways and reduce the growth and survival of breast cancer cells. Indeed, several studies have reported direct relationships between expression and activity of FASN and HER2 signaling pathway activation, which notably affected Akt and Erk activity in cancer cells -.
Chemical inhibition of de novo fatty acid synthesis decreases the proliferation of cultured breast cancer cells, reduces activation of signaling pathways, and in some cases, slows the growth of xenograft tumors ,,-; and direct inhibition of FASN has been explored for cancer therapy ,-. Clearly, modulating fatty acid synthesis activity alters tumor biology. Our study raises several important questions. Are there unique roles for MCFA versus long chain fatty acids in intracellular signaling and tumor cell proliferation? What role do dietary fatty acids play in modulating the growth of mammary tumors that have reduced FASN activity? This work highlights the potential of studying molecules that influence FASN activity as contributors to tumorigenesis and as potential therapeutic targets for the treatment of breast cancer. Furthermore, it suggests FASN activity need not be completely inhibited; instead, novel therapeutics that influence the spectrum of products produced by FASN may be sufficient to alter tumor properties such as signaling pathway activation, cell proliferation, and metastasis.
S14 enhances FASN catalysis in vitro and the S14 overexpression in the mammary epithelium during lactation facilitated the synthesis of MCFA . Here, we have demonstrated an in vivo role for S14 in modulating de novo fatty acid synthesis in two genetic mouse mammary tumor models. Our finding that altered levels of tumor MCFA are associated with changes in tumor cell proliferation, tumor metastasis, and intracellular signal transduction pathway activation of Src and Akt highlights the importance of studying those molecules that influence FASN activity as potential targets for the treatment of breast cancer. Finally, the potential relationship between S14 expression and luminal differentiation in both mouse and human tumors warrants further investigation.
This work was supported in part by DODBCRP Postdoctoral Fellowship BC098051 awarded to EAW, DOD BCRP Predoctoral Fellowship BC810596 awarded to MCR, and NIH PO1 HD38129PO1 awarded to SMA. The following Shared Resources of the University of Colorado Cancer Center, funded by NCI P30CA046934, were used in support of this research: Pathology Shared Resource and the Protein Production, Monoclonal Antibody and Tissue Culture Shared Resource. The authors would also like to thank Dr Robert Murphy and Christopher Johnson for use of their mass spectrometer and expertise, supported by Colorado Clinical and Translational Sciences Institute 5UL1RR025780, as well as Dr Aik-Choon Tan at the University of Colorado Anschutz Medical Campus for assistance with microarray data analysis.
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