The histone deacetylase inhibitor, sodium butyrate, exhibits neuroprotective effects for ischemic stroke in middle-aged female rats
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Sodium butyrate (NaB) is a histone deacetylase (HDAC) inhibitor exhibiting anti-inflammatory and neuroprotective effects in a rat ischemic model of stroke as well as a myocardial ischemia model. Although clinical evidence shows that older women are at higher risk for stroke occurrence and greater stroke severity, no studies have evaluated the effectiveness of NaB either in females or in older animals.
To determine the effects of NaB on stroke in older females, acyclic middle-aged Sprague-Dawley female rats (9–11 months old, constant diestrus) were subject to middle cerebral artery occlusion (MCAo) by intracerebral injection of recombinant endothelin-1. Rats were treated with NaB (300 mg/kg, i.p.) at 6 and 30 h following ET-1 injection. Animals were sacrificed at the early (2 days) or late (5 days) acute phase after MCAo. Serum and tissue lysates were collected for biochemical analyses.
NaB treatment reduced infarct volume and ameliorated sensory motor impairment in middle-aged female rats, when measured at 2 and 5 days post MCAo. At the early acute phase (2 days post stroke), NaB treatment decreased brain lipid peroxides, and reduced serum levels of GFAP, a surrogate marker of blood-brain barrier (BBB) permeability. NaB also reduced expression of the inflammatory cytokine IL-1beta in circulation and IL-18 in the ischemic hemisphere. At the late acute phase (5 days post stroke), NaB treatment further suppressed MCAo-induced increase of IL-1beta, IL-17A, and IL-18 in brain lysates (cortex and striatum) from the ischemic hemisphere, and decreased ischemia-induced upregulation of IL-1beta and IL-18 in circulation, indicating a potent anti-inflammatory effect of the HDAC inhibitor. Moreover, NaB treatment also increased expression of IGF-1, a known neuroprotectant, in peripheral tissue including serum, liver, and spleen at the late acute phase.
These data provide the first evidence that delayed (>6 h) NaB treatment post-stroke is neuroprotective in older female rats. Additionally, these data also show that in addition to its well-known anti-inflammatory actions, NaB may exert a biphasic effect after stroke, operating initially to reduce BBB permeability and oxidative stress in the brain, and later, elevating IGF-1 expression in peripheral tissues.
KeywordsIschemic stroke Inflammation Oxidative stress Histone deacetylase inhibitor Sodium butyrate Insulin-like growth factor-1 Cytokines Middle-aged female
Brain-derived neurotrophic factor
Glial fibrillary acidic protein
Insulin-like growth factor-1
IGF binding protein 3
Middle cerebral artery
Middle cerebral artery occlusion
Thiobarbituric reactive species
Tropomyosin receptor kinase B
Stroke is the fifth-leading cause of death in the USA and the most common cause of disability . Ischemic stroke is more prevalent in the elderly, and among this population, postmenopausal women have a higher risk for stroke occurrence, greater stroke severity, and slower recovery [2, 3]. Recent preclinical studies have accurately replicated the demographic differences in which aging female mice present increased stroke damage as compared to aging male mice ; and acyclic middle-aged female rats show greater infarct volume and behavioral deficit as compared to young female rats .
Histone acetylation and deacetylation are the two major players in epigenetic mechanisms to regulate transcription and other functions in cells including neurons, microglia, and astrocytes. Protein acetylation, catalyzed by histone acetyltransferases (HATs), at histone proteins results in an open chromatin conformation, thus stimulating transcription and activating gene expression. The other modifier, histone deacetylases (HDACs), catalyzes deacetylation of histone proteins at lysine (Lys, K) residues, hence inhibiting transcription and gene expression. The levels of histone acetylation (thereby remodeling chromatin structure) is determined by the balance between HATs and HDACs. In general, HDACs function as a transcriptional repressor to silence gene expression and induce chromatin compaction. Therefore, HDAC inhibition alters the balance towards enhancing histone acetylation, chromatin relaxation and gene expression [6, 7]. Histone hypoacetylation and chromatin compaction have been reported in rodent studies of middle cerebral artery occlusion (MCAo). A fatty acid derived HDAC inhibitor, sodium butyrate (NaB) blocks class I and IIa HDACs and is known to readily cross the BBB . NaB exhibits anti-inflammatory and neuroprotective effects in a rat ischemia model of stroke as well as myocardial infarction through multiple mechanisms including reducing infarct volume, enhancing neurogenesis, and reducing pro-inflammatory cytokines in the ischemic brain [9, 10, 11, 12, 13, 14]. Administration of NaB immediately after MCAo (and at various time points) presents neurogenic effects mediated by the BDNF-TrkB signaling pathways and anti-inflammatory effects by blocking inducible nitric oxide and COX-2 induction in a male rodent model of ischemia [11, 13].
Unfortunately, no studies have evaluated the effectiveness of the HDAC inhibitors in animal models that approximate stroke prone groups such as elderly females. Older women are more likely to suffer a stroke and have worse stroke impairment, thus the present study tested the effectiveness of delayed NaB administration in middle-aged female rats during the early and late acute phase after cerebral ischemia. The early acute phase of stroke spans minutes to hours (24–48 h) after ischemia, and is marked by excitotoxicity including increased reactive oxygen species, glutamate release, with activation of local inflammatory cells and rapid necrotic cell death of neurons [15, 16]. During the late acute phase, inflammation persists and apoptotic cell death is observed as well as repair and regeneration such as neurogenesis, angiogenesis and sprouting [17, 18]. Our data shows that NaB treatment to middle age female rats after stroke modifies key features of the acute phase of ischemic stroke.
All experimental protocols were approved by the Texas A&M University Institutional Animal Care and Use Committee. All animal care and use was conducted in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council).
Animals and estrous cycle determination
Female Sprague-Dawley rats (n = 75) were purchased from Envigo (Houston, TX) as middle-aged animals (9–11 months, 280–360 g). All animals were maintained in temperature (22 °C) and humidity (45–55%) controlled environment with a 12/12 h dark-light cycle (0700 to 1900 h). Rats were fed pelleted food (Harlan, Teklad Rodent Diet) and water ad libitum. One week after arrival, animals were subject to daily vaginal smears to determine estrous status as reported previously . Briefly, collected vaginal cells were placed on slides and cell cytology was examined. Middle-aged female rats were selected if cell cytology indicated they were in constant diestrus for at least seven consecutive days. To confirm estrus status, serum samples were collected and measured for estradiol (Cayman Chemical, MI). Serum estradiol levels in this group were below the detection limit (< 6.6 pg/ml) of the assay.
Middle cerebral artery occlusion (MCAo) and sodium butyrate treatment
MCAo was induced by intracerebral injection of endothelin-1 (ET-1) to the MCA as previously described . Animals were anesthetized (100 mg/ml/kg ketamine and 20 mg/ml/kg xylazine) and placed in a stereotaxic apparatus. ET-1 (3 microliters of 0.5 μg/μl, 600 pmol) was injected at a rate of 1 microliter per minute to the left middle cerebral artery (AP: +0.9, ML: −3.4, relative to bregma, DV: −8.5, relative to dura). After the surgery, rats were treated with NaB (Sigma-Aldrich, MO, 300 mg/kg, i.p.) at 6 and 30 h following ET-1 injection.
Animals were euthanized at the early (2 days) or late (5 days) acute phase after MCAo. Brains were quickly removed and sliced coronally at 2-mm thickness. The sections were immersed in 2% 2,3,5-triphenyltetrazolium chloride (TTC) in DPBS for 30 min at 37 °C and processed for imaging. The digitized images were used to quantify infarct volume using the Quantity One software package (Bio-Rad, CA). Infarct volume estimation was performed as reported previously . Briefly, the posterior (caudal) face of each section from three consecutive sections was analyzed in each animal. The infarct area of two adjacent slices was averaged and then multiplied by the thickness of the slice, and values across all slices were added to obtain the volume of the infarct. The volume of the ischemic zone and the total volume of non-ischemic hemisphere was measured separately, and infarct volume is reported as the ratio of the ischemic volume to the non-ischemic hemisphere.
Motor impairment following MCAo was assessed using the vibrissae-evoked forelimb placement task  and the adhesive-tape test  as described previously. The vibrissae-elicited forelimb placement test was used 3 and 2 days before and 2 and 5 days after the MCAo surgery. Animals were subject to same-side placing trials and cross-midline placing trials elicited by brushing the ipsi- and contra-lesional vibrissae against the edge of a table. During the same-side forelimb placing trials, the animal’s ipsilesional vibrissae were stimulated against the edge of a table and forelimb placing response on that side was scored by an investigator, who was blinded to experimental conditions. In the cross-midline placing trials, the animal was held gently by the upper body such that the ipsilesional vibrissae lie perpendicular to the table top and the forelimb on that side is gently restrained as the vibrissae was brushed on the top of the table to evoke a response from the contralesional limb and vice versa. Between each trial the animal was allowed to rest all four limbs briefly on the table top to help relax its muscles. Ten trials were performed during each test.
The adhesive tape test was performed 2 days before and 2 or 5 days after surgery. Two pieces of adhesive-backed foam tape (Scotch Permanent Mounting Squares, 12.7 × 12.7 mm) were used as bilateral tactile stimuli attached to the palmar surface of the paw of each forelimb. For each forelimb, the time it took to remove each stimulus (tape) from the forelimbs was recorded during three trials per day for each forepaw. Animals were allowed to rest for 1 min between sessions, and each test session had a maximum time limit of 120 s.
Protein extraction and quantification
Cortex (parietal and temporal) and striatum from the ischemic and non-ischemic hemisphere, liver, and spleen samples were collected and homogenized in lysis buffer (50 mM Tris, pH 7.4, 150 mm NaCl, 10% glycerol, 1 mM EGTA, 1 mM Na-orthovanadate, pH 10, 5 μM ZnCl2, 100 mM NaF, 10 μg/ml aprotinin, 1 μg/ml leupeptin, 1 mM phenylmethylsulfonyl fluoride in dimethylsulfoxide, 1% Triton X-100), and lysates were collected after centrifugation at 20,000g for 30 min. Protein concentrations were determined using the BCA protein assay kit (Pierce, IL) and the plates were read at 562 nm in a microplate reader (Tecan Infinite® 200 PRO).
Expression levels of a panel of inflammatory cytokine/chemokine were quantified using a rat cytokine/chemokine panel (Millipore, MA). The procedure was performed according to the manufacturer's directions. Samples, standards, and controls were added to appropriate wells in a 96-well plate as stated in the kit protocol and incubated with premixed beads at room temperature for 2 h on a horizontal orbital microplate shaker. After washes (2X), 25 μL of detection antibodies was added to each well, incubated at room temperature for 1 h on the shaker, and 25 μL of streptavidin-phycoerythrin was added to each well containing the 25 μL of detection antibodies. After 30 min of incubation at room temperature, the wells were washed (2X) and filled with sheath fluid. The plate was read on a Bio-Plex System (Bio-Rad, CA). Brain cytokines and chemokine levels were normalized to total protein concentrations.
Measurement of serum and tissue IGF-1 levels
IGF-1 levels were measured using a commercial solid phase sandwich rat ELISA kit (R&D systems, MN) as per manufacturer's instruction. Samples, standards, and controls were added to appropriate wells in a 96 well plate as stated in the kit protocol and incubated at room temperature for 2 h on a horizontal orbital microplate shaker. After wash, 100 μL of conjugate was added to each well and incubated at room temperature for 2 h on the shaker. After wash and incubation in substrate solution for 30 min, the plates were read at 450 nm in a plate reader with wavelength correction to 540 nm (Tecan US Inc., Durham, NC). Sample measurements were interpolated from the standard curve, and values from tissue lysates were normalized to total protein concentrations.
Measurement of serum and tissue IGFBP-3 levels
IGFBP-3 levels were measured using a commercial ELISA kit (Crystal Chem, IL) as per manufacturer's instruction. Samples, standards, and controls were added to appropriate wells in a 96 well plate as stated in the kit protocol and incubated at room temperature for 1 h on a horizontal orbital microplate shaker. After washes (5X), 100 μL of antibody conjugate was added to each well and incubated at room temperature for 1 h on the shaker. After washes (5X), 100 μL of enzyme conjugate was added to each well and incubated at room temperature for 15 min on the shaker. After wash and incubation in 100 μL of substrate solution for 15 min in a dark room, 100 μL of stop solution was added and the plates were read at 450 nm in a plate reader with wavelength correction to 630 nm (Tecan US Inc., Durham, NC). Sample measurements were interpolated from the standard curve, and values from tissue lysates were normalized to total protein concentrations.
Measurement of serum GFAP levels
Glial fibrillary acidic protein (GFAP) levels were measured using a commercial ELISA kit (Millipore, MA) as per manufacturer’s instruction. Samples, standards, and controls were added to appropriate wells in a 96 well plate as stated in the kit protocol and incubated at room temperature for 2 h on a horizontal orbital microplate shaker. After washes (4X), 100 μL of biotinylated anti-GFAP detection antibody was added to each well and incubated at room temperature for 1 h on the shaker. After wash, 100 μL of enzyme solution was added to each well and incubated at room temperature for 30 min on the shaker. After washes (6X) and incubation in 100 μL of substrate solution for 15 min in dark room, 100 μL of stop solution was added and the plates were read at 450 nm in a plate reader (Tecan US Inc., Durham, NC). Sample measurements were interpolated from the standard curve.
Thiobarbituric acid reactive substances (TBARS) assay
Lipid peroxidation in the ischemic and non-ischemic hemispheres was determined using the TBARS assay kit (Cayman Chemical, MI) according to manufacturer’s instructions and as described previously . Briefly, a mixture of 25 μl of sample, standard and 25 μl of SDS was prepared. To this mixture, 1 ml of color reagent was added and boiled for an hour in boiling water bath. The reaction was stopped on ice by 10 min incubation and centrifuged for 10 min at 1600g at 4 °C. The supernatant (150 μl) was loaded on a 96-well plate and absorbance was read at 540 nm in Tecan plate reader. TBARS concentration was calculated from a malondialdehyde standard curve using Magellan software and normalized to the amount of total protein.
Power analysis, using data from pilot studies and other experiments using middle aged females, estimated sample size at five. Most groups had a larger sample size, with five as the minimum. For infarct volume, a two-way ANOVA coded for treatment and day was used. For behavioral tests, a paired Student’s t test was used for each group, comparing the values obtained pre- and post-stroke. For all other comparisons an unpaired Student’s t-test or a two-way ANOVA was used. Group differences were considered significant at p < 0.05 in each case. All data presented in bar graphs are the mean ± S.E.M. from multiple determinations. Specific animal numbers used for an assay is described in each figure legend.
Sodium butyrate reduces post-stroke brain infarct volume
Percent body weight change, as an indicator of sickness and safety of NaB treatment, was also measured during 24 h and 5 days post stroke (Fig. 1c). The extent of body weight change was significantly affected by treatment [F (4, 46), 4.46, p = 0.0409] (Fig. 1c). Relative body weight change showed that the percent weight loss post MCAo was significantly reduced in NaB-treated animals (−2.013 ± 0.52%, n = 13) compared to control (−5.112 ± 0.63%, n = 14) during 24 h after MCAo (Fig. 1c).
Sodium butyrate treatment ameliorates stroke-induced loss of sensory motor function
NaB reduces blood brain barrier permeability in the early acute phase
NaB reduces lipid oxidation in brain in the early acute phase
HDAC inhibitors including NaB have shown to attenuate oxidative damage in animal models of bipolar disorder, diabetes, and cancer [28, 29, 30, 31]. To assess the effect of NaB on MCAo induced oxidative stress, we measured the levels of thiobarbituric reactive species (TBARS), as a proxy marker of reactive oxygen species [32, 33]. As shown in Fig. 4b, c, at 2 days post stroke TBARS values display a main effect of treatment (NaB) in both hemispheres (Fig. 4b, p = 0.0025) whereas at 5 days there was no differences among the treatment groups (Fig. 4c, p = 0.2210), indicating that NaB promotes an early protective effect on ischemia-induced oxidative stress in brain.
NaB-mediated neuroprotection after stroke is associated with the anti-inflammatory effect in circulation and brain
The protective effect of NaB against ischemia is associated with elevated levels of insulin-like growth factor-1 (IGF-1)
IGF-binding protein-3 (IGFBP-3) is increased in ischemic hemisphere as compared to non-ischemic hemisphere post stroke
Our data provide the first evidence that post-stroke NaB treatment is neuroprotective in middle-aged reproductive senescent female rats. In this study, we show that two i.p. injections (6 and 30 h after MCAo) of NaB significantly reduced cortical and striatal infarct volume and ameliorated stroke-induced loss of sensory motor function. Preclinical studies have identified NaB as a potential therapeutic drug for ischemic stroke [11, 40, 41], although these studies have mainly utilized young male animals. While older women have a higher risk for stroke and poorer recovery as compared to aged men, no studies have evaluated the effectiveness of HDAC inhibitors in clinically relevant animal models such as aged animals or females. Furthermore, few studies have treated NaB at a delayed time point (>6 h) following stroke, which is a critical question for stroke therapy . The present study convincingly demonstrates that delayed NaB administration is effective for middle-aged female rats after cerebral ischemia.
While NaB is generally shown to improve stroke outcomes, its mechanism of action appears to be pleiotropic. During the acute phase poststroke, NaB reduces oxidative stress and blood-brain barrier permeability, whereas, during the delayed phase poststroke, NaB promotes cell survival and tissue repair and recovery [43, 44]. At the early phase (ranging from minutes to hours), reactive oxygen species are released from injured cells, further stimulating the release of inflammatory cytokines as well as matrix metalloproteinases, which act in concert to increase blood brain barrier permeability and trafficking of leukocytes [15, 16]. Delayed actions of NaB may contribute to stroke recovery and repair in two ways: one, by elevating IGF-1 in periphery tissues, which may trigger remodeling responses in both endothelial cells and astrocytes [22, 45] and additionally, by decreasing pro-inflammatory cytokines such as IL-17. IL-17 is produced by gammadeltaT lymphocytes, and infiltration of this T cell cohort in the brain during the acute phase of stroke been shown to promote infarction and brain damage . Decreased IL-17 production in NaB group is thus consistent with the reduced infarct volume seen in this group and reducing this inflammatory cytokine may further promote repair processes in delayed phase of stroke.
Unlike its action on lipid peroxides and IGF-1, NaB suppression of inflammatory cytokines spanned the early and late acute phase of stroke. Our data show that NaB decreased pro-inflammatory cytokine levels of IL-1beta in circulation at 2 days post stroke and both IL-18 and IL-1beta at 5 days post stroke. In the ischemic hemisphere, NaB decreased IL-18 at 2 days post stroke and IL-1beta, IL-17a, and IL-18 at 5 days post stroke. Our previous studies show that neither of these proteins are significantly different in young or middle aged females , and are likely driven by ischemic injury. Proinflammatory cytokines released during ischemic stroke have a detrimental effect on neuronal survival and functional recovery [47, 48]. Lower levels of IL-18 by NaB at both 2 and 5 days post stroke is particularly interesting in view of the data that this cytokine is reported to have prognostic value in patients with acute ischemic stroke [49, 50, 51]. IL-18 is synthesized peripherally by macrophages and human peripheral blood mononuclear cells [52, 53] and by microglia, astrocytes, and neurons in central nervous system as the first response of immune defense [54, 55, 56]. Zaremba and colleagues have shown that serum IL-18 level is significantly elevated in stroke patients as compared to controls, and IL-18 levels were negatively correlated with both the Scandinavian Stroke Score and the Barthel Index . Moreover, circulating IL-18 levels from blood drawn 48 h after ischemic stroke was a major predictor of 90-day major adverse clinical outcomes in this cohort . These clinical reports are consistent with our observation in middle-aged female rats where higher IL-18 levels in the saline-treated group is accompanied by worse sensory motor function as compared to the post-stroke NaB-treated group. In addition to the clinical data, preclinical studies using myeloid cells have shown that HDAC inhibitors including NaB, valproic acid and TSA increase expression of IL-18 mRNA .
We also found lower levels of IL-17A by NaB in the ischemic hemisphere only at later acute phase of stroke (5 days, Fig. 6e), but not at early acute phase (Fig. 6b), while in peripheral tissues lower levels of IL17A were found both at 2 and 5 days (Fig. 5b). This may imply migration of Th17 cells to the brain at later acute phase of stroke, which is initially activated from periphery at the earlier acute phase. It is plausible because HDAC inhibitors are reported to regulate T cell polarization, by which HDAC inhibition suppresses the polarization toward the pro-inflammatory Th17 cells . Our data also suggest that NaB protect the brain from neuroinflammatory responses, in part, by suppressing peripheral immune responses by reducing trafficking of T-cells.
While the effects of NaB on inflammatory cytokines are extensively studied, its effects on IGF-1 are not well known. The IGF-1 signaling pathway is a critical biological modulator of cellular growth, development, and metabolism . Our laboratory has previously demonstrated that stroke severity in older females is associated with decreased IGF-1 availability  and IGF-1 treatment, provided intracerebroventricularly, after stroke protects the aging brain by reducing blood brain barrier disruption and neuroinflammation . Interestingly, while NaB significantly increases IGF-1 in peripheral tissues in the post stroke animal, there was no elevation of IGF-1 in the brain with this treatment. In a study examining the biodistribution of exogenous fatty acids including n-butyric acid (BA), 4-phenylbutyric acid (PBA) and valproic acid in primates , only a very small amount (<0.006%) is taken up by the brain, whereas majority of these fatty acids were taken up by spleen (BA) and liver (PBA). Considering this biodistribution, it is not surprising that NaB was most effective in IGF-1 level in liver, spleen, and serum. The relationship between NaB and IGF-1 is poorly understood. NaB has been shown to cause a dose dependent increase in IGFBP3 and IGFBP2 protein and mRNA , proteins that are known to store and increase the half-life of IGF-I. Since the dose of NaB used in this study has been reported to restore MCAo-induced decrease in histone acetylation , another likely possibility is that increased IGF-1 expression pathway may result from increased transcription due to preservation of histone acetylation.
Stroke-induced inflammation, and the trafficking of immune cells to the ischemic brain is a critical feature of stroke pathophysiology. Young, cycling females typically display small infarct volumes with fewer T cells in the brain after stroke , which is positively correlated with serum estradiol levels. Loss of this ovarian hormone in aged females was associated with increased stroke outcome and splenic contraction. In a comprehensive analysis of the central and peripheral immune response to MCAo, Zhang et al. reported that in estrogen-deficient females, stroke increased splenic Treg cells and reduced splenocyte proliferation and thymocyte numbers, while estrogen replacement reduced infarct volume, decreased splenic Treg cell numbers and increased splenic proliferation . These data suggest that the loss of estrogen in middle aged females likely exacerbates the immune cell invasion to the brain and causes worse outcome post stroke. It is also consistent with the idea that NaB may improve infarct volume in this population by modulating the inflammatory response.
While aged women are likely to have a higher risk for stroke, worse outcomes and poorer recovery after the event compared to aged men, preclinical studies routinely failed to utilize clinically relevant animal models, such as aged female model. The present study suggests that readily supplementable and clinically well-tolerated NaB could be an effective treatment to improve sensory motor function and outcome after acute ischemic stroke in middle-aged females, suggesting that HDAC inhibitors might have utility in treating acute stroke in a broad range of demographics. While several studies show that sodium butyrate is neuroprotective, the precise mechanism is unclear, and is likely to be multi-pronged, involving anti-inflammatory, anti-apoptotic and growth factor mediated pathways . The current study shows that NaB may target peripheral organs to promote central nervous system health.
The results of the present study with a HDAC inhibitor, sodium butyrate (NaB) provide the first evidence that delayed NaB treatment post-stroke is neuroprotective in middle-aged reproductive senescent female rats. Our data indicate that two doses of NaB by i.p. injection (6 and 30 h after MCAo) significantly reduce corticostriatal infarct volume and significantly attenuates loss of sensory motor function. The neuroprotection of NaB against MCAo is associated with an anti-inflammatory effect of this HDAC inhibitor. Additionally, our data show that NaB also an early anti-oxidant effect and a later trophic effect, specifically, elevating IGF-1, which we have previously shown is a robust neuroprotectant for stroke in aging females.
The authors would like to thank Dr. Amutha Selvamani (Texas A&M University Health Science Center) for the excellent guidance and technical assistance on behavioral assays.
This work was supported by NIH/NS074895 and AG042189 to FS and a Women’s Health in Neuroscience (WHIN) Fellowship to MJP.
Availability of data and material
All of the data is provided in the manuscript.
FS and MJP conceived and designed the experiments. MJP performed the experiments. MJP and FS analyzed the data. MJP and FS wrote the paper. Both authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
All experimental protocols involving the use of animals were approved by Texas A&M University Institutional Animal Care and Use Committee. All animal care and use was conducted in accordance with the Guide for the Care and Use of Laboratory Animals (National Research Council).
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