Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus
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Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) has led to serious economic losses to the swine industry worldwide. In this study, an oral recombinant Lactobacillus casei vaccine against PEDV infection targeting the intestinal microfold (M) cells and dendritic cells (DCs) for delivering the core neutralizing epitope (COE) of PEDV spike protein was developed with M cell-targeting peptide (Col) and dendritic cell-targeting peptide (DCpep). The immunogenicity of the orally administered recombinant strains was evaluated.
After immunization, significantly higher levels of anti-PEDV specific IgG antibodies with PEDV neutralizing activity in the sera and mucosal sIgA antibodies in the tractus genitalis, intestinal mucus, and stools were detected in mice orally administered with the recombinant strain pPG-COE-Col-DCpep/L393, which expressed DCpep and Col targeting ligands fused with the PEDV COE antigen, compared to mice orally immunized with the recombinant strain pPG-COE/L393 without the DCpep and Col targeting ligands. Moreover, in response to restimulation with the PEDV COE antigen in vitro, a significant difference in splenocyte proliferation response and Th2-associated cytokine IL-4 level was observed in the group of mice orally immunized with pPG-COE-Col-DCpep/L393 (p < 0.05) compared to the groups of mice that received pPG-COE-Col/L393 and pPG-COE-DCpep/L393, respectively.
KeywordsLactobacillus Porcine epidemic diarrhea virus Dendritic cell-targeting peptide Microfold cell-targeting peptide Oral immunization
Porcine epidemic diarrhea (PED) characterized by vomiting, diarrhea, and dehydration, is a highly contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV). PEDV, a member of the Coronaviridae family, is an enveloped, single-stranded RNA virus whose genome comprises five open reading frames (ORFs) encoding an accessory protein and four structural proteins . Of these, the spike on the surface of PEDV mediates binding and fusion between the virus and cells. In addition, the neutralizing epitope region of PEDV (COE) has been shown to induce virus neutralization antibodies and is used as a potential candidate immunogen against PEDV [2, 3]. PEDV has caused serious economic losses to the swine industry in Europe, America, Australia, and especially Asia. Currently, commercial vaccines, which mostly include inactivated vaccines  and attenuated PEDV vaccines, have provided some protection, but their efficacy has been poor [5, 6]. Since PEDV causes mainly intestinal infections , secretory IgA (sIgA), which can bind microbes and toxins in the intestine to prevent their adherence to epithelial cells , is desirable for defending the mucosa against PEDV. Thus, research on vaccination inducing a more efficacious sIgA-based protective mucosal immunity is urgently needed to prevent PEDV infections.
Oral mucosal vaccination has the great advantage of improving practicality for mass vaccination during pandemics, along with ease of production and administration. Above all, local protective mucosal immunity which is pivotal in response to PEDV invasion is generally not induced with parenteral vaccination . Induction of mucosal immunity should be focused on the interaction between the antigen and lymphocytes within the mucosa. One promising approach involves the use of live recombinant Lactobacillus which can colonize the gastrointestinal tract and compete with pathogens for mucosal binding sites . Cell wall associated or secreted factors from some Lactobacillus strains can enhance innate immune responses and epithelial barrier function, modulate the intestinal micro-environment, regulate immune cell behavior, and elicit release of cytokines . In addition to natural immune-stimulating adjuvants with weak immunogenicity  and capacity to survive the gastric acid and digestive enzymes, several Lactobacillus strains have been applied for the delivery of heterologous antigens to trigger mucosal immune responses against pathogens [13, 14, 15].
Although a live recombinant Lactobacillus vaccine can propagate in the porcine intestine, only a small amount of antigen can reach the effective immune sites such as Peyer’s patches (PPs) . To increase the bioavailability of the immunogen of interest, the addition of dendritic cell-targeting peptide (DCpep) and microfold cell (M cell)-targeting peptide (Col) to this oral Lactobacillus delivery system has been proposed. Microbial sampling commonly includes two principal mechanisms: (1) M cells, predominantly localized at PPs can transfer the antigen to DCs and macrophages for processing and (2) DCs pierce the epithelial layers to sample the antigen directly . Previous studies have demonstrated that DCpep directs the protective antigen to DCs using Lactobacillus as a vector to induce the desired intestinal and systemic immune response [18, 19, 20]. Furthermore, M cell ligand-fused antigens have been shown to enhance the specific mucosal immune response [21, 22]. A combination of targeting to DCs and M cells simultaneously may further strengthen and improve the antigen uptake and presentation.
Therefore, in the present study, genetically engineered Lactobacillus casei 393 (L. casei 393, L393) strains, were constructed to constitutively express the COE of PEDV with either one or both of DCpep and Col on their cell surface. The immunogenicity of these recombinant strains was then investigated by oral administration in mice.
Virus, bacteria, plasmids, and cell line
PEDV HLJ-2012 was isolated from the intestines of piglets with severe diarrhea in Heilongjiang, China, and was maintained in our laboratory. Lactobacillus casei ATCC 393 was cultured in de Man Rogosa and Sharpe (MRS) broth at 37 °C. The constitutive expression plasmid pPG-T7g10-PPT, previously constructed by our laboratory, contained the HCE strong constitutive promoter, T7g10 transcriptional enhancer, PgsA anchor from Bacillus subtilis for stabilizing the heterologous protein in the cell membrane (surface-displaying), and the rrnBT1T2 terminator , and was used to construct COE plasmids as described below.
Construction of recombinant Lactobacillus strains
Sequence of primers
GGGCCCTTATGGACGTTGTGGAGTTGAATGATATGATGGATAAAA a TGAGCCACCGCCACC b TGGTAATGGTGAACGAGCTGGTAATTGATGAAATGA c TGAACCTCTAGAAACGTCCGTGACACCTTC
Analysis of protein expression by western blotting and immunofluorescence
Briefly, 2 mL of recombinant L. casei 393 was grown in MRS broth containing chloramphenicol (10 μg mL−1) at 37 °C for 12 h (OD600 ≈ 1), harvested by centrifugation, and washed twice with sterile PBS. After lysis of the sedimented cells with lysozyme, the lysate was incubated with sodium dodecyl sulfate (SDS) loading buffer in a boiling water bath for 10 min. The bacterial proteins were then separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and electrotransferred onto a polyvinylidene fluoride (PVDF) membrane. The immunoblots were probed with a mouse anti-COE serum (1:200) prepared in our laboratory as the primary antibody, and a horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:4000) as the secondary antibody.
Furthermore, for detection of the surface-displayed COE and targeting peptide of recombinant L. casei, immunofluorescence was used as described previously. In brief, recombinant strains were cultured overnight in MRS at 37 °C. The cell pellets were subsequently incubated with anti-PEDV COE serum (1:100) followed by fluorescein isothiocyanate-conjugated goat anti-mouse IgG (Invitrogen, USA) secondary antibodies (1:1000). Samples were then washed thrice with PBS, stained with 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen, USA) for 30 min at 4 °C, washed three times, resuspended in 200 μL PBS, and then smeared on a microscope slide. Images were viewed by laser confocal microscopy (model LSM510 META; Zeiss, Germany).
Six-week-old female specific pathogen-free (SPF) BALB/c mice were purchased from Liaoning Changsheng Biotechnology Company in Liaoning, China, and kept under SPF conditions with free access to standard water and diet. All animal procedures were approved by the Ethical Committee for Animal Experiments (Northeast Agricultural University, Harbin, China). Prior to oral administration, the recombinant Lactobacillus strains were cultured overnight in MRS medium, washed with sterile PBS, and resuspended in PBS at a concentration of 1010 CFU mL−1. Sixty mice were randomly divided into six groups (10 mice per group): PBS, pPG/L393, pPG-COE/L393, pPG-COE-Col/L393, pPG-COE-DCpep/L393, and pPG -COE-Col-DCpep/L393 groups. The immunization dosage was 100 μL (109 CFU) per mice, administered on 3 consecutive days (days 1, 2, and 3). Booster immunizations were administered on days 14, 15, and 16.
Serum and mucosal lavage samples from the external genital tract were obtained on days 0, 10, 17, 27, 34, 41 after immunization. Immunized mice (five mice in each group) were sacrificed to collect the intestinal tract (from duodenum to ileum) before the primary immunization. And five mice were sacrificed on day 40 after immunization to collect the intestinal tract and spleens, followed by washing with sterile PBS. Samples of the intestinal tract were stored at − 40 °C until analysis. In addition, fecal samples were collected on days 0, 4, 6, 8, 10, 12, 14, 16, 20, 22, 24, 26, 28, 30, 32, 34, and 36 after immunization, and treated according to a previously described method . Briefly, 0.1 g of fecal pellets were suspended in 400 μL of PBS containing 1 mmol L−1 phenylmethylsulfonyl fluoride (Sigma, USA) and 1% bovine serum albumin (BSA), and then incubated at 4 °C for 16 h. After centrifugation, the supernatants were stored at − 40 °C until use.
ELISA analysis of antibody levels
The levels of IgG in the sera and IgA in the external genital tract, intestinal tract, and stools were measured by ELISA. Polystyrene microtiter plates were coated overnight at 4 °C with PEDV NJ propagated on Vero cells and the culture of Vero cells used as a negative control for the antigen. After blocking with 5% skimmed milk, the collected samples were serially diluted in PBS, added in triplicate and incubated at 37 °C for 1 h. Then, an HRP-conjugated goat anti-mouse IgG or IgA antibody (Invitrogen, USA) was added to each well (1:5000) and incubated for 1 h at 37 °C. Color was then developed using o-phenylenediamine dihydrochloride (Sigma, USA) as a substrate, and absorbance at OD490 was measured.
Proliferation of lymphocytes from immunized mice and cytokine ELISA
On day 40 post-immunization, splenocytes were obtained from immunized mice subjected to euthanasia for lymphocyte proliferation assay. In brief, 100 μL of the cell suspension (5 × 106 cells mL−1), in eight duplicates, four of which were prepared for cytokine ELISA, were incubated in a 96-well plate containing RPMI 1640 medium plus 10% fetal calf serum at 37 °C in a 5% CO2 incubator. The cells were then restimulated with 5.0 μg mL−1 concanavalin A (ConA), 0.5 or 5.0 μg mL−1 of purified recombinant COE protein, and culture medium for 72 h. Control wells contained medium alone. Lymphocyte proliferation was assessed by a CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay according to the manufacturer’s instructions (Promega, USA), with absorbance measured at 570 nm. The stimulation index was calculated as follows: SI = OD570: sample/OD570: blank control.
Supernatant fluids (from the lymphocyte proliferation assay) were collected at 72 h, after which IL-4 and IFN-γ assays were performed using ELISA kits, according to the manufacturer’s instructions (Biosource International, USA). The cytokine concentrations were calculated according to the standard curve obtained for each ELISA plate.
Briefly, sera from mice on day 41 post-immunization were filtered and inactivated at 56 °C for 30 min. The sera were serially diluted twofold (1:2, 1:4, 1:8, 1:16, 1:32, 1:64……), mixed with an equal volume of virus suspension (200 TCID50), and incubated at 37 °C for 1 h. The intermixture was inoculated onto a Vero cell monolayer at 37 °C in a 5% CO2 incubator. The positive serum control, negative serum control, virus control, and blank control were prearranged. Cytopathic effect (CPE) was observed daily. The Reed–Muench statistical method was used to calculate the results .
Data are shown as the mean ± standard errors of three replicates per test in a single experiment repeated three times. Tukey’s multiple comparison tests were used to analyze differences between groups. A p value less than 0.05 was considered statistically significant, and a p value less than 0.01 was considered highly significant.
Expression of the fusion protein
Immune responses induced in mice by oral administration of the recombinant strains
Proliferation of lymphocytes and cytokine ELISA
The neutralizing activity of sera from mice orally administered with the recombinant L. casei was evaluated by diluting antibodies and mixing with a maintained dose of the virus. Neutralization titer was calculated by the Reed–Muench statistical method. The neutralization titer of PBS and pPG/L393 groups was less than 1:2. The titers for PPG-COE/L393 (1:6), pPG-COE-Col/L393 (1:24), pPG-COE-DCpep/L393 (1:24), pPG-COE-Col-DCpep/L393 (1:36) indicated that mice administered with L. casei expressing COE-Col-DCpep possessed a stronger ability to neutralize the PEDV than ones administered with L. casei expressing COE-Col or COE-DCpep. The neutralizing activity of sera from COE-Col and COE-DCpep groups are similar. Recombinant strains with targeting ligand groups conferred a higher level of neutralization on PEDV infection compared with pPG-COE/L393. Moreover, no neutralizing activity was observed in the sera of mice administered with pPG/L393 or PBS.
Currently, PEDV has caused significant economic losses because of high mortality and loss of productivity. Subsequently, attenuated and inactivated PEDV vaccines have been developed [28, 29]. For reasons unknown, some vaccines based on classical PEDV strains have failed to control the more recent virulent PEDV strains in Asia . The evolution of developing effective vaccines against PEDV has never ceased due to the presence of epidemic and variant strains. The S protein of coronaviruses is reported to be responsible for binding to cellular receptors in the early steps of infection and induces neutralizing antibodies in vivo . Immunogens based on local virus isolates may help to prevent and control the outbreaks in this region. In this study, the gene homology of COE (499–638 amino acids in S protein) between classical PEDV strains and HLJ-2012 strain was 93.6–98.8%, and the COE cloned in this study did not show any insertion or deletion in its gene sequence.
Parenteral vaccination generally fails to induce mucosal immunity where PEDV infection is localized. Oral vaccination can stimulate a protective mucosal immune response as well as be crucial for passive lactogenic immunity . A recent study suggested that successful generation of PEDV neutralizing antibodies in milk depends on oral immunization or oral exposure of sows, and that serum IgG antibody may not be a correlative immunity for PEDV . IgG was dominant in the colostrum, but was decreased in sow milk with time. SIgA accounts for 40% of the total milk antibodies . In order to deliver COE to the gastrointestinal tract by oral administration, genetically engineered lactobacillus constitutively surface-displaying COE was constructed as a particulate antigen for induction of immune responses and immunocyte memory. L. casei 393 can survive in bile salts or low pH in the gastrointestinal tract as well as colonize the intestines of mice and swine for a few days  to enhance the systemic and mucosal immune responses for orally administered antigens . Constitutive expression without an inducer is generally preferred for in situ delivery of antigens by lactobacillus in consideration of productive stability and the high cost of inducers.
One of the essential considerations for oral vaccines is that the immunogen needs to penetrate the gut wall where epithelial cells are lined tightly, to be taken up by antigen presenting cells, which influence the immune efficiency. Antigen sampling occurs by transcytosis in the membrane of M cells, as well as in the dendrite extensions of dendritic cells into the lumen . In view of this, L. casei 393 strains that expressed mammalian DCpep and Col fused separately or together with the immunogen for targeted vaccination were evaluated for their immune efficiency in mice. Theoretically, recombinant lactobacillus first adheres to the intestinal mucus competing for binding epitopes with pathogenic bacteria. Simultaneously, the recombinant Lactobacillus was guided by Col binding to the receptor of M cells to be imported into the dome area of the gut associated lymphoid tissue where DCs are present. Then DCpep allows COE to be captured by DCs by binding to its receptors. Moreover, COE fused with DCpep can be directly engulfed by DCs through their dendrites between the epithelial cells. Protective immunity can also be elicited by activated B cells secreting sIgA [37, 38] and activated T cells differentiation. DCs can present processed COE to T and B cells directly or migrate into the mesenteric lymph nodes for antigen presentation. DC-activated T and B cells can migrate to the periphery to induce specific immunity against the pathogen challenge . Our data showed that recombinant strains with targeting ligands, which likely recruited DCs and M cells, promoted more rapid and stronger immune responses at the mucosal and systemic levels, compared with those induced by pPG-COE/L393.
In this study, COE-Col, COE-DCpep, and COE-Col-DCpep groups showed higher sIgA levels in the feces compared with the COE group, from the sixth day post immunization, and higher IgG and sIgA in the tractus genitalis from day 10 post immunization, which illustrates that in the early stages, targeting ligands can help elicit better immunogenicity more rapidly, especially sIgA levels, indicating the significance of our oral vaccine. To evaluate the effect on cellular immunity, splenocyte proliferation and cytokine levels were determined. Differential effects on specific cell-mediated immunity depend on the adjuvant dosage, Lactobacillus strains, and antigen used [39, 40]. Higher levels of Th2-associated IL-4 and Th1-associated IFN-γ in vaccine groups than those in the control group illustrated cellular immune response was induced. The trend of splenocyte proliferation and IL-4 levels indicate that targeting ligands may enhance the production of memory lymphocytes and humoral immunity (COE-Col-DCpep group > COE-Col group > COE-DCpep group > COE group > PBS group, p < 0.05). Other relevant researches also demonstrate that targeting peptides can enhance antigen-specific IgG, IgA, T cell responses [19, 21]. Of these, it is reported that the antibody levels from mice orally administrated Lactobacillus expressing antigen-DCpep, were comparable with that from mice in the group vaccinated with antigen-adjuvant (single subcutaneous injection) . Another research showed that the Co1 ligand did not induce oral tolerance and the use of Co1 resulted in a skewed Th2-type immune response . In addition, we evaluated neutralizing antibodies, which can neutralize the viral pathogen by binding to epitopes on antigens associated with virus neutralization. After neutralization, some parts of the virus’s infection cycle were inhibited, including cellular surface binding, fusion, entry, endocytosis, and replication . Neutralizing antibodies have provided a quality correlate of vaccine efficacy for many licensed vaccines . Assessment of PEDV-neutralizing activity illustrated that the COE-Col-DCpep group has the best neutralization potential suggesting that pPG-COE-Col-DCpep/L393 may be a promising vaccine candidate against PEDV infections.
The mucosal immune system properly balances pathogen surveillance and tolerance to dietary antigens and commensal microbes. And this indicates that mucosal antigens are generally less immunogenic than antigens delivered by other route. Potent adjuvants and delivery platforms are required for effective mucosal vaccination [43, 44]. It is reported that DCpep and Co1 would reduce the need for adjuvants to enhance immune responses and have mucosal adjuvant ability [9, 18, 21]. In this study, L. casei 393, which can colonize the intestine transiently, was applied as delivery vector and potent adjuvant themselves. Moreover, targeting ligands can favorably increase the bioavailability of the vaccine and help to elicit mucosal and systemic immune responses.
Further studies should focus on swine zoopery to investigate immune efficacy and protection. Although the BALB/c mouse is not a susceptible animal model for PEDV, our results to some extent indicate that surface-displaying COE-Col-DCpep L. casei could serve as a novel mucosal vaccine that provides opportunities for PEDV vaccine development.
We used L. casei 393 as an antigen carrier to deliver COE fused with M cell and DC targeting ligands as an oral vaccine. Our results showed that pPG-COE-Col-DCpep/L393, pPG-COE-Col/L393, pPG-COE-DCpep/L393, were able to effectively induce immune responses at both the mucosal and systemic levels compared with pPG-COE/L393. Moreover, we found that L. casei 393 delivering the COE antigens in combination with DCpep and Col can promote stronger, more rapid antigen-specific immune responses in mice, suggesting it as a promising strategy for vaccine development against PEDV infection.
CS, WXN, SW performed gene cloning, electrotransformation, expression characterization (western blot and FACS). HXW, WL, QXY and JYP presided over laboratory animals, performed all immunizations, collected samples and ELISA. MST, TLJ, XYG, LYJ analyzed data and wrote the paper. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Availability of data and materials
All the data analyzed have been included in this article.
Consent for publication
Ethics approval and consent to participate
Animal experiments were carried out in accordance with the recommendations in the institutional and national guidelines for animal care and use. The protocol was approved by the Committee on the Ethics of Animal Experiments of Northeast Agricultural University, Harbin, China (2016NEFU-315, 13 April 2017). All procedures were performed under ether anesthesia, and made to minimize suffering.
This research was supported by the National Key Research and Development Program of China (2016YFD0500100), the National Natural Science Foundation of China (31672591, 31472226) and the National Science and Technology Support Program in Rural Areas of the12th Five-Year Plan (Grant 2015BAD12B02-7).
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