Drawing the line
The pea aphid Acyrthosiphon pisum is an agriculturally important pest of leguminous plants including peas and broad beans. The widespread use of chemical pesticides impacts heavily on the environment, and increases pesticide-resistant pea aphid populations, so alternative strategies are being actively sought. Pseudomonas syringae bacteria are known to infect and kill the pea aphid, and offer a possible control strategy. In this study, the authors measured the effects of injecting P. syringae on the survival of pea aphid populations at 24 and 48 hours. The pea aphid population was killed more rapidly (98 % after 24 hours) with a higher concentration of injected bacteria than in the control or with lower concentrations, indicating that a P. syringae-based control strategy may be a useful alternative to conventional pesticides.
KeywordsBacterial Culture Line Graph Percent Survival Critical Difference Broad Bean
However, drawing a line through just three points, as the authors have done, is unlikely to be an accurate representation of the relationship between percent survival and hours post-injection. It may be that the survival of insects injected with the bacteria at an OD of 10 decreased more rapidly; for example, the graph indicates that survival was 45 % after 12 hours, but it could equally have been 20 %, which is a considerably shorter time frame than this graph suggests. This could affect the authors’ conclusions: in this case, the critical difference may not be the total number of aphids killed by 24 hours post-infection, but how many were killed within 6 or 12 hours, as pea aphids can very rapidly cause damage on crop plants. More data from intermediate time points are clearly required to describe the relationship.
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