Escherichia coli porcine peritonitis induces histological and transcriptome evidence of cardiac injury
- 1.5k Downloads
KeywordsCardiac Output Peritonitis Cardiac Dysfunction Infected Animal Post Infection
Cardiac dysfunction is a feature of sepsis. In order to gain insight into the fundamental mechanisms of this phenotype, gene expression analysis (Affymetrix) was applied to serial cardiac biopsies of sham (n = 2) and E. coli infected pigs (n = 3).
Cardiac samples were taken basal and hourly after infection for gene analysis and at the end of the experiment for histopathological examination. Genes were determined to be differentially regulated at a greater than or less than twofold change and P < 0.05.
These results will provide biomarker and mechanistic insights to pathogenesis of cardiac dysfunction of septic peritonitis and may also help identify some altered novel gene transcription pathways that can serve as new targets for diagnostic tools and therapeutic strategies. All candidate genes will be validated by quantitative PCR.