Inhibition of ABA-induced stomatal closure by fusicoccin is associated with cytosolic acidification-mediated hydrogen peroxide removal
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Fusicoccin (FC), a fungal phytotoxin produced by Fusicoccum amygdale, causes the inhibition of ABA-induced stomatal closure. The mechanism of inhibition is remaining unclear. We analyzed the role of hydrogen peroxide (H2O2) and relationship between H2O2 removal and cytosolic pH changes during inhibition of ABA-induced stomatal closure by FC.
According to the results, ABA treatment induced H2O2 production and stomatal closure, but FC inhibited the effects of ABA on these two parameters. Treatment with catalase (CAT) and NADPH oxidase inhibitor diphenylene iodonium (DPI) mimicked the effect of FC. These data suggest that inhibition of ABA effect by FC is related to the decrease of H2O2 levels in guard cells. Furthermore, similar to CAT, FC not only suppressed stomatal closure and H2O2 levels in guard cells treated with exogenous H2O2, but also reopened the stomata which had been closed by ABA and reduced the level of H2O2 that had been produced by ABA, indicating that FC causes H2O2 removal in guard cells. The butyric acid treatment simulated the effects of FC on the stomatal aperture and H2O2 levels in guard cells treated with exogenous H2O2 and had been closed by ABA, and both FC and butyric acid reduced cytosolic pH in guard cells of stomata treated with H2O2 and had been closed by ABA, which demonstrate that cytosolic acidification mediates FC-induced H2O2 removal.
These results suggest that FC causes cytosolic acidification in guard cells, then induces H2O2 removal and reduces H2O2 levels in guard cells, finally inhibits stomatal closure induced by ABA.
KeywordsAbscisic acid Cytosolic acidification Fusicoccin Guard cell Hydrogen peroxide
2′,7′-bis(2-carboxyethyl)-5(6)-carboxy fluorescein-acetoxy methyl ester
Abscisic acid (ABA) is a phytohormone that plays vital roles in the control of growth and development and is involved in the response to various environmental stresses. ABA has been demonstrated to affect leaf size, shoot growth, stomatal and lateral root development (Parent et al. 2009; Finkelstein et al. 2002; Arend et al. 2009; Lenoble et al. 2004; de Smet et al. 2003). Moreover, it’s generally known ABA as a stress signal in plants. Drought and high salinity resulted in strong increases of plant ABA levels, accompanied by a major change in gene expression and in adaptive physiological responses (Christmann et al. 2007; Rabbani et al. 2003; Zeller et al. 2009). ABA has been shown to induce stomatal closure and reduce the loss of transpirational water from plants under drought conditions (García-Mata and Lamattina 2001; Luan 2002). A large number of ABA signaling intermediates has been identified in guard cells, including cytosolic calcium, protein kinases, cADPR, G proteins and ion channels (Hamilton et al. 2000; Schroeder et al. 2001; Leckie et al. 1998; Wang et al. 2001). Moreover, phospholipid sphingosine-1-phosphate (SIP), phospholipase C and nitric oxide (NO) have also been suggested to involve in ABA signaling pathways in stomatal closing movement (Hetherington 2001; Ng et al. 2001; Bright et al. 2006; Zhang et al. 2007).
Fusicoccin (FC), a fungal phytotoxin produced by Fusicoccum amydali, stimulates several physiological and biochemical processes, such as cell elongation, breaking of seed dormancy, ethylene production, stomatal opening and solute transport (Marrè 1979; Malerba et al. 1995). Since several lines of evidence obtained both in vivo and in vitro proved that FC is a powerful activator of the plasma membrane (PM) H+-ATPase, most of the effects induced by the toxin in the plant tissues have been ascribed to the activation of this transport system (Marrè 1979; Beffagna et al. 1977; Palmgren 1998). It is now widely accepted that FC activates the H+-ATPase by binding to a regulatory protein belonging to the 14-3-3 family, whose association with a specific binding sequence located at the end of the C-terminal autoinhibitory domain of the PM H+-ATPase releases the autoinhibitory action (Beffagna and Lutzu 2007). FC binding promotes and stabilizes this association, releases the autoinhibitory action and thus induces the activation of H+-ATPase (. Olsson et al1998; Svennelid et al. 1999; Kinoshita and Shimazaki 2001).
Previous studies demonstrate that ABA induces H2O2 production and partially blocks both blue light- and FC-dependent activation of H+-ATPases by decreasing in phosphorylation of H+-ATPase via H2O2 (Schroeder et al. 2001; Zhang et al. 2004; Goh et al. 1996), and cytosolic alkalinization is an early step preceding the production of reactive oxygen species (ROS) in the ABA-triggered signal cascade in guard cells (Suhita et al. 2004; Gonugunta et al. 2008; Islam et al. 2010; Gehring et al. 1997). However, Irving et al. reported that acidification of guard cell cytosol by kinetin, IAA or FC preceded stomatal opening (Irving et al. 1992), H+-ATPase could be activated by FC powerfully (Marrè 1979; Beffagna et al. 1977), and a decrease of endogenous H2O2 levels were associated with auxins- and cytokinins-induced stomatal opening (Song et al. 2006). These data indicate an opposite action of FC and ABA on cytosolic pH, H+-ATPase activity and stomatal movement, and it remains unclear whether or not this effect is related to the changes of H2O2 levels. In the present work, we found that the inhibition of ABA-induced stomatal closure by FC involves a decrease in H2O2 levels in guard cells of Vicia faba, and the decrease of H2O2 levels is mediated by cytosolic acidification.
Molecular probes 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA) was obtained from Biotium (Hayward, CA). The fluorescence probes of 2′,7′-bis(2-carboxyethyl)-5n-carboxy fluorescein-acetoxy methyl ester (BCECF-AM), ABA, fusicoccin (FC), catalase (CAT, from bovine liver), diphenylene iodonium (DPI), DMSO, Pluronic F-127, MES and butyric acid were purchased from Sigma-Aldrich (St Louis, MO). Unless stated otherwise, the remaining chemicals were of the highest analytical grade available from various suppliers of Chinese companies.
Broad bean (Vicia faba L.) was grown in a controlled-environment plant growth chamber with a humidity of 80%, a photon flux density of 300 μmol m-2 s-1PAR generated by cool white fluorescent tubes (Philips, New York, NY), and an ambient temperature 25 ± 2°C with a 14-h light and 10-h dark cycle. The epidermis was peeled carefully from the abaxial surface of the youngest, fully expanded leaves of 4-week-old seedlings, and cut into pieces about 5 mm width and 5 mm lengths.
Stomatal apertures were monitored by the method of McAinsh et al. (McAinsh et al. 1996) with slight modifications. To study the effects of FC, CAT and DPI on stomatal closure caused by ABA, freshly prepared abaxial epidermal strips were incubated in CO2-free MES/KCl buffer (10 mM MES/KOH, 50 mM KCl, 100 μM CaCl2, pH 6.15) with FC, CAT or DPI for 3 h with ABA under light conditions (300 μmol m-2 s-1) at 25 ± 2°C. Final stomatal apertures were recorded with a light microscope and an eyepiece graticule previously calibrated with a stage micrometer. To study the effects of FC, CAT and butyric acid on stomatal closure caused by exogenous H2O2, epidermal strips were incubated in MES/KCl buffer with H2O2 alone, or containing FC, CAT and different concentrations of butyric acid for 3 h, and then the stomatal apertures were recorded. To study the effects of FC, CAT and butyric acid on stomata that had been closed by ABA, strips were incubated in MES/KCl buffer for 3 h with ABA and were then treated with fresh buffer alone, or containing FC, CAT and different concentrations of butyric acid for another 1 h, final stomatal apertures were recorded.
To avoid any potential rhythmic effects on stomatal aperture, experiments were always started at the same time of the day. In each treatment, we scored 30 randomly selected apertures per replicate and treatments were repeated three times. The data presented are the means of 90 measurements ± s.e.
Dyes loading of H2DCF-DA and BCECF-AM
The epidermal strips were treated as described for stomatal bioassay section, and were then loaded with 50 μM H2DCF-DA (10 min) or 20 μM BCECF-AM (10 min), in Tris–KCl loading buffer (Tris 10 mM and KCl 50 mM, pH 7.2) containing 0.05% Pluronic F-127 in the dark at 25 ± 2°C. In experiment involving time-course monitoring of H2O2 levels and cytosolic pH in guard cells, the epidermal strips were treated with ABA for 3 h and then with FC for another 10, 20, 30, 40, 50, or 60 min before loading with probes.
Laser-scanning confocal microscopy
After excess dye was washed off with fresh Tris–KCl loading buffer in darkness, TCS SP5 laser-scanning confocal microscopy (Leica Lasertechnik Gmbh, Heidelberg, Germany) was used to measure cytosolic pH or H2O2 content in guard cells of Vicia faba (excitation 488 nm, emission 505–530 nm, power 15%, PMT 959, pinhole 0.000036, zoom ~4, normal scanning speed, frame 512 × 512 pixels). Images acquired from the confocal microscope were analysed with Leica image software and Photoshop. To enable the comparison of changes in signal intensity, confocal images were taken under identical conditions (in manual setup) for all samples, and in each treatment we measured three epidermal strips, and the treatment was repeated three times. The selected confocal images represented the same results from three replications.
Statistical analyses were performed by using a one-way ANOVA followed by the least significant difference (l.s.d.) test.
FC inhibits stomatal closure caused by ABA and reduces ABA-induced H2O2 levels in guard cells
Widely researches showed that ABA-induced stomatal closure is related to the production of endogenous H2O2 (Suhita et al. 2004; Pei et al. 2000; Murata et al. 2001). To know if there is a relationship between the inhibition of ABA-induced stomatal closure by FC and the levels of H2O2 in guard cells, the strips were treated with CAT (a H2O2 scavenger) and DPI (an inhibitor of H2O2–generating enzyme NADPH oxidase), respectively. The results show that CAT significantly inhibited ABA-induced stomatal closure in a dose-dependent manner (Figure 1B), and DPI suppressed ABA-induced stomatal closure partially (Figure 1C), indicating that H2O2 is required for ABA-induced stomatal closure and NADPH oxidase contributes to H2O2 production, which is consistent with the results reported previously (Zhang et al. 2001). The optimal concentration CAT and DPI on stomatal aperture were 100 units mL-1 and 10 μM, respectively. These results suggest that, probably like CAT and DPI, FC inhibition of ABA-induced stomatal closure via decreasing H2O2 levels in guard cells.
Both FC and butyric acid suppress exogenous H2O2-induced stomatal closure and DCF fluorescence in guard cells
To further clarify whether FC can affect exogenous H2O2-induced DCF fluorescence, the epidermal strips were treated with H2O2 in the presence of FC for 3 h, and then H2O2 levels were measured. As shown in Figure 3B, a striking DCF fluorescence in guard cells was observed after treatment with 100 μM H2O2. Compared with the control, there were no changes of DCF fluorescence in guard cells treated with FC alone (Figure 3B). However, H2O2-induced DCF fluorescence in guard cells was largely prevented by FC (Figure 3B). Similarly, CAT also substantially suppressed exogenous H2O2-induced DCF fluorescence (Figure 3B). DPI had no obvious effect on fluorescence (Figure 3B). These results show that like CAT, FC really induces H2O2 removal, and consequently reduces H2O2 level in guard cells treated with exogenous H2O2.
Previous study demonstrated that FC causes guard cells cytosolic acidification (Irving et al. 1992). These results prompt us to explore whether or not guard cells cytosolic acidification mediates H2O2 decrease induced by FC. For this purpose, we studied the effects of butyric acid on exogenous H2O2-induced stomatal closure and H2O2 levels in guard cells treated with exogenous H2O2. As shown in Figure 3C, butyric acid at the concentration of ≥ 0.5 mM significantly suppressed exogenous H2O2-induced stomatal closure (P < 0.05) (Figure 3C) and markedly reduced H2O2 contents in guard cells treated with exogenous H2O2 (P < 0.05) (Figure 3D). The results indicate that cytosolic acidification really promotes the removal of H2O2 within guard cells, thereby preventing H2O2-induced stomatal closure.
Both FC and butyric acid reopen the stomata had been closed by ABA and reduce the level of H2O2 had been generated by ABA in guard cells.
The effect of FC on the level of H2O2 that had been generated by ABA in guard cells was also measured in the present studies. After an incubation of 3 h in MES/KCl buffer in ABA, the strips were treated with fresh MES/KCl buffer alone, or containing FC and CAT for another 1 h, and then were loaded with H2DCF-DA, washed, and examined by laser scanning confocal microscopy. As shown in Figure 4B, compared with the control, both FC and CAT largely abolished the DCF fluorescence of guard cells. These results suggest that, the treatment of FC induces H2O2 removal, hence reduces the level of H2O2 induced by ABA in guard cells.
To further explore whether or not cytosolic acidification is related to the removal of H2O2 in guard cells, the effects of butyric acid on the stomata had been closed by ABA and the level of H2O2 had been generated by ABA were measured. Figure 4 shows that, butyric acid at the concentration of ≥ 0.5 mM evidently reopened the stomata had been closed by ABA (P < 0.05) (Figure 4C), and markedly reduced the level of H2O2 had been generated by ABA (P < 0.05) (Figure 4D). The results provide evidence that cytosolic acidification assuredly induces H2O2 removal in guard cells, and thus reopens the stomata had been closed by ABA.
Both FC and butyric acid reduce cytosolic pH in guard cells treated with exogenous H2O2 and stomata had been closed by ABA
Guard cells control transpiration in plants and regulate gas exchange in leaves by opening and closing stomatal pores. Stomatal opening is induced by many abiotic and biotic factors, including light, indoleacetic acid (IAA), FC, cytokinins, low CO2 levels and high humidity (Mansfield and Atkinson 1990; Jewer and Incoll 1980; Pemadasa 1982; Braunsgaard et al. 1998), and stomatal closure can be promoted by dark, osmotic stress, high CO2 concentrations, decreased humidity and ABA (Schroeder et al. 2001; Kearns and Assmann 1993). Previous studies demonstrated that cytosolic alkalinization of guard cell precedes ROS production and is required for ABA- and MJ-induced stomatal closure (Suhita et al. 2004; Islam et al. 2010; Gehring et al. 1997; Gonugunta et al. 2009). FC, IAA and kinetin decreased the cytosolic pH and promoted stomatal opening (Irving et al. 1992), and a decrease of endogenous H2O2 levels were associated with auxins- and cytokinins-induced stomatal opening (Song et al. 2006). These results suggested that cytosolic pH was an important factor in the regulation of H2O2 levels and stomatal movement. However, until recently, little was known whether the inhibition of ABA-induced stomatal closure by FC is related to the change of cytosolic pH and H2O2 levels in guard cells. The results of the present studies demonstrate that similar to CAT and DPI, FC significantly prevented stomatal closure induced by ABA (Figure 1) and largely reduced H2O2 levels in guard cells induced by ABA (Figure 2). The results suggest that the inhibition of ABA-induced stomatal closure by FC is associated with a decrease of H2O2 levels in guard cells.
Previous reports demonstrated that FC stimulates production of H2O2 in cultured sycamore and Arabidopsis thaliana cells (Beffagna and Lutzu 2007; Malerba et al. 2003). However, FC was also reported to block cryptogein-induced H2O2 production of tobacco cells (Simon-Plas et al. 1997). The results of the present study show that the inhibition of ABA-induced stomatal closure by FC is associated with a decrease of H2O2 levels in stomatal guard cells. The question arises of how FC reduces H2O2 levels in guard cells. Our results show that FC suppressed exogenous H2O2-induced stomatal closure and H2O2 levels in guard cells treated with exogenous H2O2 (Figure 3A,B), and also reopened the closed stomata by ABA and abolished H2O2 that had been generated by ABA (Figure 4A,B). The above-mentioned effects of FC are similar to those of CAT (Figures 3A,B, and 4A,B), a scavenger of H2O2. These results prove that the treatment with FC induces the removal of H2O2 within guard cells, thereby prevents stomatal closure induced by exogenous H2O2, and causes the reopening of the closed stomata by ABA. Together with the facts that FC inhibits stomatal closure induced by ABA (Figure 1A) and reduces H2O2 levels in guard cells caused by ABA (Figure 2D), we conclude that FC probably initiates an unidentified mechanism, which can reduce H2O2 levels in guard cells via inducing H2O2 removal, eventually preventing stomatal closure induced by ABA.
Cytosolic pH is an important factor in the regulation of stomatal movement (Suhita et al. 2004; Gonugunta et al. 2008; Irving et al. 1992; Blatt 2000; Zhang et al. 2001). Cytosolic alkalinization is a major step in the ABA-triggered signal cascade in guard cells leading to stomatal closure (Irving et al. 1992; Blatt 2000), and further investigation found that cytosolic alkalinization preceded the production of ROS and NO during ABA induced stomatal closure (Suhita et al. 2004; Gonugunta et al. 2008, 2009). In contrast, FC, IAA or a weak acid butyrate, decreased the cytosolic pH and promoted stomatal opening (Irving et al. 1992), and auxins also reduce H2O2 levels (Song et al. 2006). These results prompted us to investigate the interaction of pH and H2O2 levels in FC-inhibited stomatal closure. In this study, we provide evidence that, similar to butyric acid, FC not only reduced cytosolic pH caused by ABA in guard cells (Figures 5 and 7) but also induced H2O2 removal (Figures 2 and 4), which suggests that cytosolic acidification in guard cells mediates H2O2 removal induced by FC. Real-time monitoring with the help of fluorescent dyes BCECF-AM and H2DCF-DA revealed that FC-induced decrease of cytosolic pH faster than that of H2O2 levels and confirmed that acidification of guard cell could be upstream of H2O2 during inhibition of ABA-induced stomatal closure by FC (Figure 8). Combined with the fact that FC reduces cytosolic pH (Figure 5D) and H2O2 levels (Figure 2D) in guard cells induced by ABA, we conclude that FC induces H2O2 removal via reducing cytosol pH, hence lessens H2O2 levels in guard cells during ABA-induced stomatal closure. It is an intriguing problem about how FC-induced cytosolic acidification causes H2O2 removal, and we suppose that some H2O2-scavenging mechanism might be activated, including change of ASA redox state, catalase or ascorbate peroxidase activities (Beffagna and Lutzu 2007; Chen and Gallie 2004).
In summary, these data suggest that FC induces H2O2 removal and reduces H2O2 level via reducing cytosol pH in guard cells, thus inhibiting ABA-induced stomatal closure.
This work was financially supported by National Natural Science Foundation of China (31000130) and Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education (zs12006).
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