Regulation of osmoadaptation in the moderate halophile Halobacillus halophilus: chloride, glutamate and switching osmolyte strategies
- 13k Downloads
The moderate halophile Halobacillus halophilus is the paradigm for chloride dependent growth in prokaryotes. Recent experiments shed light on the molecular basis of the chloride dependence that is reviewed here. In the presence of moderate salinities Halobacillus halophilus mainly accumulates glutamine and glutamate to adjust turgor. The transcription of glnA2 (encoding a glutamine synthetase) as well as the glutamine synthetase activity were identified as chloride dependent steps. Halobacillus halophilus switches its osmolyte strategy and produces proline as the main compatible solute at high salinities. Furthermore, Halobacillus halophilus also shifts its osmolyte strategy at the transition from the exponential to the stationary phase where proline is exchanged by ectoine. Glutamate was found as a “second messenger” essential for proline production. This observation leads to a new model of sensing salinity by sensing the physico-chemical properties of different anions.
KeywordsGlutamate Glutamine Synthetase Compatible Solute Glycine Betaine Glutamine Synthetase Activity
Chloride, an abundant anion in nature
Together with flourine, bromine, iodine and astatine, chlorine belongs to the seventh group in the periodic table of elements, the "halogens". Chloride is one of the most abundant anions on earth and its content can make up to 75% in non-saline soil  or 90% in saline soils  of all inorganic anions. Huge amounts of chloride can also be found in marine habitats where its concentration can reach up to 550 mM (in combination with the corresponding cation Na+). This represents about 95% of the solvated anions. Due to this ubiquitous availability it is reasonable to assume that chloride is involved in biological processes.
Chloride is essential for some extremely halophilic prokaryotes for osmoregulation
Halophilic organisms are abundant in nature and thrive in habitats where molar concentrations of salts (mainly NaCl) are present and, consequently, very low water potentials exist. However, every living cell is dependent on the availability of liquid water and therefore has to counterbalance the external osmotic conditions. To serve this purpose, and despite the different osmotic demands, only two main strategies have evolved in nature.
A very abundant strategy to counteract the loss of water under hyperosmotic conditions is the accumulation of compatible solutes [3, 4, 5, 6]. Compatible solutes are per definition small, organic molecules that are soluble in water to molar concentrations and which do not interfere with cell metabolism . Although a great spectrum of compatible solutes is used in the three domains of life, almost all can be assigned to two chemical classes: (i) the amino acids and their derivatives, such as glycine betaine, glutamine, glutamate, proline, ectoine or N-acetyl-β-lysine [8, 9, 10, 3, 11, 12, 13, 14, 15] and (ii) the sugars and polyols, such as trehalose, glycerol, di-myo-inositol-1,1'-phosphate (DIP) or sulfotrehalose [16, 17, 18, 19, 20, 21, 22]. The accumulation can be accomplished either by uptake from the medium or by de novo synthesis.
Contrary to the accumulation of compatible solutes, some halophilic, anaerobic Bacteria (Halanaerobiales) and some Archaea (Halobacteriales) accumulate KCl to molar concentrations to counterbalance the external salinity [23, 24, 25, 26, 27]. Although almost nothing is known about the mechanism of KCl accumulation in Halanaerobiales, the process is well understood in Halobacteriales. The electrical potential (Δψ) that drives the uptake of potassium ions in these organisms results from the concerted action of the membrane bound proton-pump bacteriorhodopsin and the "proton gradient-consuming" proteins ATP synthase and Na+/H+ antiporter . Potassium is taken up via a K+ uniport mechanism. To enable such a transport the electrical potential (Δψ) has to be greater than the diffusion potential of K+ (Δψ K). The counterion chloride is taken up either by primary or secondary transporters . In the dark, a light-independent Cl-/Na+ symporter is employed , but only little is known so far about this transport mechanism and the transporter is unknown. A far better understood transport of Cl- is accomplished via the light-driven chloride pump halorhodopsin . Halorhodopsin is a heptahelical membrane protein and together with bacteriorhodopsin and sensory rhodopsins belongs to the subfamily of archaeal rhodopsins [32, 33]. To its only lysine residue (Lys242) a retinal is bound covalently as a protonated Schiff base . This Schiff base, together with the surrounding amino acids, forms the active centre of the chloride pump, which is able to absorb green light with a maximal wavelength of 578 nm. The absorption of one photon triggers a photochemical reaction that leads from all-trans to a 13-cis configuration. This isomerization reaction is coupled to the transport of one molecule of Cl- from the surrounding medium into the cell [34, 35].
To date, only one representative of the aerobic bacteria is known that also shares the strategy of KCl accumulation to cope with extreme salinities. The extremely halophilic Salinibacter ruber was isolated from saltern crystallizer ponds in Spain  and was reported to need a minimal salt concentration of 1.7 M to allow growth. The optimal salinity was found between 2.5 and 3.9 M NaCl. It accumulates very high concentrations of Cl- and K+/Na+, while other solutes were found only in minor concentrations . Although biochemical analyses addressing the uptake of K+ and Cl- are not yet available, it was concluded from genome analyses that potassium could be taken up via a TrkHA transport mechanism. S. ruber possesses 4 copies of a putative trkA and two copies of a putative trkH gene that seem to originate from different sources via lateral gene transfer . TrkH is the membrane spanning translocating subunit and TrkA is the cytoplasmic membrane surface protein that binds NADH/NAD+ and which is essential for the transport activity . Similar to the halobacteria, chloride could be taken up by the means of the chloride pump halorhodopsin. Altogether 4 putative genes encoding a rhodopsin were identified in the genome. Two of which were, based on sequence similarity, assigned to be sensory rhodopsins, one to be a proton pump and one a chloride pump . Additionally, two copies of Na-K-Cl cotransporter – genes were identified that are common in eukaryotes but seldom found in prokaryotes. The physiological role of such a transporter is unknown for prokaryotes, but eventually contributes to the accumulation of K+ and Cl- in S. ruber cells .
As a prerequisite for this so called "salt-in-cytoplasm" strategy these organisms have a highly adapted, salt-tolerant or even salt-dependent enzyme equipment, that shows a high amount of acidic and a low amount of aromatic amino acid residues compared to non halophilic organisms [40, 23, 41, 42]. This adaptation, however, sets clear restrictions on the habitat in which these organisms can exist, since the proteins always need a quite high intracellular salt concentration for correct protein folding and activity . However, it should be mentioned that this is rather a tendency than a rule. It was reported for Haloferax volcanii (Halobacteriales), for example, that this organism is able to adapt to salinities between 1 M and almost saturation [43, 44], but nothing is known about the mechanism of osmoregulation in H. volcanii.
Halobacillus halophilus– the paradigm for chloride dependent processes in moderately halophilic bacteria
A comparison of the proteome of nitrate and chloride grown cells revealed 5 additional proteins that were specifically produced in the presence of chloride. Among those proteins, which were tentatively identified by BLAST searches, members of the general stress regulon, as described for B. subtilis [51, 52], were found. One was SodA, a superoxide dismutase, another YvyD, a modulator of the sigma factor σL. Furthermore, an N-acetylmuramoyl-L-alanine amidase that is essential in cell wall biosynthesis, a protein with unknown function and a protein (LuxS) that is essential in the production of autoinducer-2 or in the activated methyl cycle were found . For the latter the proteome data were confirmed by additional studies, which not only revealed a chloride dependent production of LuxS, but also showed a clear influence of chloride on luxS transcription . In sum, the data obtained from proteome analysis suggest the existence of a global regulatory network of processes that are dependent or at least are strongly influenced by chloride .
The main compatible solutes in Halobacillus halophilusand their biosynthesis
Since Cl- is indispensable for growth it has to influence a growth essential process. The nature of this process was unidentified for a long time. For a moderate halophile a definitely essential function is to measure external salinities and to adapt to changing osmolarities in the habitat. Halobacillus halophilus, as already mentioned, was isolated from salt marshes. The salt marshes represent an elevated, coastal area that is flooded during high tides with sea water that possesses an average salt concentration in the North Sea of about 3.5%. The salt is mainly NaCl. Upon evaporation and additional salt agglomeration by the sea wind the salt concentrations can rise up to 10% in the salt marshes, but drop almost to the level of freshwater after rainfall. The first hint that chloride could be involved in osmoregulation and the accumulation of compatible solutes came with investigations published by Roeßler and Müller in 2001. Transport studies with radiolabelled glycine betaine revealed a strict sodium and chloride dependence of glycine betaine uptake as response to osmotic upshock. Inhibitor studies suggested a primary transport system . In the absence of glycine betaine, Halobacillus halophilus copes with the effect of external salt by accumulating of a cocktail of different compatible solutes, mainly amino acids such as glutamine, glutamate, proline and alanine but also of amino acid derivatives such as Nε-acetyl lysine, Nδ-acetyl ornithine, glycine betaine and ectoine . The obvious question that arose from these considerations was whether the accumulation of compatible solutes via de novo biosynthesis was also a chloride-dependent process and whether this was the key to an explanation for the chloride dependence of Halobacillus halophilus. To address this question intensive biochemical and physiological studies were done to solve the pathways for compatible solute biosynthesis, to solve their regulation and to clarify the role of chloride in these processes.
The biosynthesis of glutamate and glutamine
For the biosynthesis of glutamate and glutamine three main enzymatic reactions are known in biology. The biosynthesis of glutamate can either be accomplished by the action of a glutamate synthase (GOGAT) or by the action of a glutamate dehydrogenase (GDH) while glutamine is synthesized by the action of a glutamine synthetase (GS).
Glutamine is synthesized by the action of a glutamine synthetase in Halobacillus halophilus . In the genome two open reading frames could be identified each encoding a putative glutamine synthetase. While the first one (glnA1) is clustered with a gene (glnR) encoding a regulatory protein with very high similarity to the regulator GlnR of B. subtilis that is known to be essential in nitrogen metabolism, the second one (glnA2) lies solitary and is preceded by a putative promoter that shares very high similarity to the promoter of B. subtilis that is recognized by σB, the general stress σ-factor. Analysis of mRNAs of cells cultivated at different salinities (0.4 – 3.0 M NaCl) revealed a strongly salinity-dependent expression of glnA2 with a maximal increase of transcripts of about 4-fold (compared to the value at 0.4 M NaCl) at 1.5 M NaCl or higher. Expression of glnA1, however, was not influenced by different salinities. The existence of two genes for the same enzyme can be regarded as a simple means for regulation that makes it feasible to separate and process completely different physiological demands: nitrogen-metabolism and osmoregulation.
A glutamine synthetase activity was readily detectable and it was apparent that this activity increased with increasing salinity of the medium . Interestingly, maximal activity was not found at intermediate salt concentrations as expected from the mRNA data and the glutamate concentration measured in the cells, but was found at 2.5 M NaCl or higher. This observation does not necessarily reflect a contradiction, but rather underlines the role of glutamine, but also glutamate, that can easily be made of glutamine, as precursor molecules for other building blocks and finally compatible solutes like proline or ectoine at higher salinities, as discussed later.
Expression of glnA2 appeared to be affected only slightly, but significantly by the presence or absence of the anion chloride. This is perfectly in line with previous studies, where regulation on the transcriptional level was shown to be of minor importance in Halobacillus halophilus [50, 53]. A more striking influence of chloride was found on the level of glutamine synthetase activity, which appeared to be strictly chloride dependent. The way how chloride can influence the activity of the glutamine synthetase is not elucidated yet, but principally two different modes of regulation are conceivable. First, and this is the easiest one, chloride could directly bind to the enzyme and influence its stability and/or conformational state. It is known from previous work that chloride is available within the cell  and putative basic residues to which chloride could bind were identified in the protein sequence of the Halobacillus halophilus glutamine synthetase. Second, the glutamine synthetase could be regulated by a regulatory protein that directly or indirectly senses the concentration of chloride. To clarify this question, future efforts have to be focused on the chloride dependence of purified glutamine synthetase and the question of how different anions can bind and influence the activity of the glutamine synthetase. However, the finding of a chloride dependent compatible solute production, for the first time, gave a rationale for the chloride dependence of Halobacillus halophilus, since growth in saline environments is only feasible with a functional active osmoregulation.
The biosynthesis of proline
Unlike B. subtilis , no other genes were found that encode for potential proline biosynthesis enzymes and, therefore, the genes/proteins identified in Halobacillus halophilus have to respond to different stimuli, such as low proline-content within the cell and high salinity. Analyses of proHJA transcript levels, where the molecular answer to an increase in salinity was addressed, revealed an almost immediate increase of mRNA after an osmotic upshock that reached a maximum already 1.5 hours after the upshock . At the same time the proline concentration increased and reached a maximum after 6 hours. This result demonstrated that salinity triggers the production of proline, but left open the question on the actual signal that leads to the increase in proline production preferentially at high salinities. A clue what the actual signal could be came up when the same experiment was performed with Na-glutamate instead of NaCl. Interestingly, glutamate also led to an accumulation of proline, but in contrast to NaCl the addition of Na-glutamate dramatically increased the proHJA mRNA concentration. The stimulating effect of glutamate on the transcription of the pro genes was subsequently titrated and it was found that a minimal concentration of 0.2 M glutamate is sufficient to stimulate pro gene transcription . Following these data it was speculated that not (only) NaCl is the initial signal that triggers the production of proline, but the internal glutamate concentration, that increases with increasing salinity. Glutamate is therefore regarded as a "second messenger" in Halobacillus halophilus beside of being a compatible solute.
Based on these data it is not surprising that glutamate is able to substitute chloride in growing cells . Glutamate was shown to be taken up and accumulated in a chloride-independent manner. Within the cell it then serves as the major compatible solute and as a "second messenger" leading to enforced transcription of several genes involved in osmoregulation, for example such genes as glnA2 or proHJA. Moreover, it can be assumed that transcription of additional genes is also stimulated by the presence of glutamate and, therefore, growth is allowed in the absence of chloride. However, it should be kept in mind, that 1 M Na-glutamate is artificial and not encountered in the ecosystem. Interestingly, glutamate cannot short-circuit the Cl- – regulon in general. The biosynthesis of flagellin and therefore motility cannot be achieved in the absence of chloride but presence of glutamate.
The biosynthesis of ectoine
Sequence of events in osmoadaptation in Halobacillus halophilus
Time-dependent (temporal) response
Adaptations in stationary growth-phase
Cells, however, that were situated for a prolonged time at high cell densities replace proline (at least in part) by ectoine . The ectoine biosynthesis and final concentration thereby depend on the salinity of the medium and the availability of chloride. Again, glutamate is crucial and serves as a NH2-donor in this pathway (Fig. 5). The mode of how Halobacillus halophilus cells sense the density of the culture is currently not known, but could be accomplished for example via quorum sensing. Halobacillus halophilus produces autoinducers of the furanone-type (autoinducer 2 [AI-2]) that accumulate in stationary phase cultures (Saum, Winzer, Müller unpublished data). Moreover, the transcription and translation of the key-enzyme for AI-2 production was shown to be chloride dependent as already mentioned above.
The chloride regulon of Halobacillus halophilus
Concomitantly with the identification of the growth-essential process of osmoregulation as a chloride dependent process, the question about the molecular basis for chloride as a regulator molecule in this process comes up. In this context one has to keep in mind that before solutes are accumulated as a response to hyperosmolarity the cell has to sense osmolarity somehow. The process of sensing osmolarity is generally only poorly understood and almost not investigated in moderate halophiles. Studies with the halotolerant model organism E. coli lead to the assumption that the initial signal that is sensed after an osmotic upshock is the reduced turgor pressure that establishes due to a loss of water. This reduced turgor leads to an influx of K+ within seconds via the potassium transporters Kup, Trk, Kdp [73, 74] but also activates the KdpD-KdpE regulatory system which in turn regulates the expression of the kdpFABC operon encoding the Kdp transport system. The KdpD-KdpE system is a classical, bacterial regulatory two-component system with KdpD being the membrane-bound sensor kinase and KdpE being the response regulator. The actual mode of sensing the turgor pressure that leads to the autophosphorylation of KdpD is still a matter of debate, but was postulated to be due to alterations of the transmembrane helix-helix interactions, but additionally seems to be influenced by the K+ concentration [75, 76, 29].
Contrary to this model, the osmosensing two-component system MtrB-MtrA of Corynebacterium glutamicum does not respond to a reduced turgor pressure after osmotic upshock. Instead, the addition of different chemical compounds, like sugars, amino acids or polyethylene glycols seems to trigger the autophosphorylation activity of MtrB. The proposed explanation for this is the change in the hydration state of the protein caused by the solutes . In Halobacillus halophilus two-component systems are present, but none of them could definitely be assigned to be of the KdpDE or the MtrBA type. However, the actual signal that is recognized as well as the mechanism might be different in different organisms.
However, not only two-component systems may serve as potential osmo-sensors, but also transporter proteins. BetP of C. glutamicum or E. coli is a Na+/glycine betaine symporter of the betaine-carnitine-choline transporter family (BCCT) that is strongly regulated by the osmolality of the medium. The regulatory domain was shown to be located in the C-terminus that extends into the cytoplasm. Due to its basic characteristics it is believed to interact with the cytoplasmic membrane, but loses its interaction upon K+ accumulation after osmotic upshock. The loss of interaction in turn leads to a conformational change within the protein and to an increase of specificity for its substrate glycine betaine [78, 79, 80].
A similar mechanism of "sensing" the surface charge of the membrane was proposed for the ATP-hydrolyzing ATP-transporter OpuA of Lactococcus lactis. OpuA is a high-affinity transporter for glycine betaine that effectively can be switched on or off by osmotic upshift or downshift, respectively . The molecular sensors with which the osmotic strength can be measured were identified to be the C-terminal CBS domains of the ATP-hydrolyzing subunits. They possess a very anionic character and were therefore postulated to be influenced by the negatively charged membrane. Similar to BetP the change of the ionic strength could result in a conformational change and therefore activation of the transporter .
In contrast to the enteric bacteria, like E. coli for example, or the Gram-positive bacterium C. glutamicum, that have to be able to respond to fluctuating osmolality rather than salinity, the major osmolyte Halobacillus halophilus is confronted with, is NaCl. It would therefore be an attractive explanation that Halobacillus halophilus senses changing salinities by sensing changing chloride concentrations. The actual mechanism is unclear to date, but several models are conceivable: (i) Chloride could be measured via an extracellular receptor. The binding of substrate would then trigger a signal transduction cascade into the cell similar to a two component system. The disadvantage of this model would be that it cannot explain how the intracellular osmotic state is measured. (ii) Another concept could be based on the idea that changes in salinity could be measured via a chloride transport process into the cell. The import could be measured by a specific transporter or by an associated protein which then leads to a signal transduction cascade and finally to a cellular response. A similar model was already proposed for the moderately halophilic bacterium Halomonas elongata where the transport of the compatible solute ectoine is thought to be the osmosensor . (iii) A chloride-binding regulator in the cytoplasm could directly interact with the anion and could, depending on the chloride concentration, trigger cellular responses. One putative candidate for such a function is the regulator protein YvyD that was identified in chloride-but not in nitrate-grown Halobacillus halophilus cells . The latter two models have the advantage that they provide a possible mechanism, with which changes in the external salinity – that are reflected in the intracellular chloride concentrations – can be measured. However, the fact that very different processes are affected by the presence or absence of chloride in Halobacillus halophilus demands the presence of a global regulatory system. The crucial point of these concepts is that they cannot be explained by the presence of only one regulatory protein, since no regulator is known to date that is able to regulate on such different targets as transcription, translation and enzyme activity at the same time. In Halobacillus halophilus no direct evidence was found so far, with exception of YvyD, that a multi component regulatory network of chloride dependent regulators exists.
Physico-chemical effects of (an)ions on biological systems
Based on the Hofmeister series and the effects of anions on proteins or whole cells, a different concept can be proposed for Halobacillus halophilus to measure osmolarity. Halobacillus halophilus is a moderately halophilic organism that has adapted to saline environments. Since chloride is not excluded from the cytoplasm but rather accumulates, depending on the external salinity, to molar concentrations within the cell , it is principally thinkable that the physical effects caused by the presence of chloride is of regulatory importance. A prerequisite for the usage of such a strategy is the evolutionary development of proteins that are functional in the presence of such high ion concentrations. As a consequence such enzymes might be dependent on the presence of minimal Cl- concentrations for optimal functionality. Examples for such a behavior were already found in Halobacillus halophilus in the case of the glutamine synthetase that demands an optimal salinity of 1.5 M NaCl . Such an adaptation to high salinities is already observed in extremely halophilic organisms such as members of the Halobacteriaceae or Salinibacter ruber and it would be in good correlation with the identification of 7 positively charged residues in the deduced protein sequence of GlnA2 – the postulated chloride dependent glutamine synthetase – at which chloride could bind . The Hofmeister series not only offers an explanation of the regulatory influence of chloride on cell metabolism in Halobacillus halophilus but also offers an explanation to the regulatory influence of glutamate in the process of pro-gene transcription. Under physiological pH glutamic acid is deprotonated to the anion glutamate- that accumulates within the cell to molar concentrations. Since too high concentrations of such anions are inhibitory for cell metabolism, the cell has to substitute glutamate- by an electroneutral compatible solute like proline. The same mechanism was previously proposed for E. coli that substitutes glutamate- by trehalose . Again, the cell has to "sense" the concentration of an anion (in this case glutamate-). For Halobacillus halophilus it was shown that a minimal external glutamate concentration of 0.2 M is sufficient to stimulate pro gene transcription very effectively . Following these data it was speculated that not (only) NaCl is the initial signal that triggers the production of proline, but also the internal glutamate concentration, that increases with increasing salinity. Glutamate is therefore regarded as a "second messenger" in Halobacillus halophilus beside of being a compatible solute. This idea was nicely corroborated by studies done in the group of Prof. Gralla that demonstrate the potential of glutamate as an activator or inhibitor of transcription [96, 97]. In sum, based on the Hofmeister series a very sophisticated model of regulatory sequences can be proposed for the osmoregulation in Halobacillus halophilus that is solely based on physico-chemical properties of anions.
An intermediate strategy for a moderate halophile
A still open question is why Halobacillus halophilus additionally accumulates compatible solutes to molar concentrations although there are high concentrations of chloride present in the cell (Fig. 1). The advantage of such a hybrid strategy (salt-in and compatible solute in) lies at hand. Strictly halophilic archaea are dependent on molar concentrations of NaCl in their environment and exclusively use the salt-in strategy to counterbalance the low water activity. Although single members of the haloarchaea are known to be able to grow over a broad range of salinity (H. volcanii for example), they still depend on minimum NaCl concentration of 1.5 M or higher to grow. This dependence is due to a very pronounced adaptation of the whole enzymatic machinery on high salt concentrations. Such a radical adaptation would be counterproductive for a moderate halophile like Halobacillus halophilus thriving in habitats where salinities temporarily can reach molar concentrations but also can fall to freshwater concentrations after rainfalls. Instead, an only modest adaptation to elevated salinities on the protein level would be advantageous. It could help the cell to tolerate a sudden or dramatic increase of salinity that concomitantly would result at least in a temporary increase of salt concentration in the cytoplasm, easier, since enzymes are still active. However, such an adaptation on the enzyme level requires the presence of a minimal salinity outside and within the cell for optimal operation and could therefore explain the chloride concentrations measured in Halobacillus halophilus. The nature of the cation that balances the negative charge of chloride is not yet known, but could be K+. An only modest adaptation to elevated salinities within the cell also explains the need for alternative osmoprotectants at high external salinities. Therefore, Halobacillus halophilus has to accumulate compatible solutes such as glycine betaine, glutamate, glutamine, proline or ectoine in addition.
In summary, a dual strategy in osmoprotection is able to explain the broad range of tolerated salt concentrations that is common to all moderately halophilic organisms. In the context of this model Halobacillus halophilus represents an intermediate in respect to its demands of NaCl between the non halophilic and the extreme halophilic organisms. It is therefore conceivable that this organism employs an intermediate strategy of salt-in-cytoplasm and the use of compatible solutes.
The model of Halobacillus halophilus as an organism that uses an intermediate strategy for osmoregulation and physico-chemical properties of anions to regulate the processes of compatible solute accumulation opens a whole new field of regulatory mechanisms to study. Future investigations will have to show for example whether chloride directly influences the activity of the glutamine synthetase or if the stimulation of activity is mediated by a so far unknown factor. Further, the role of glutamate will have to be investigated in more detail. For this purpose in vitro experiments have to be established to study its effect on transcription. Also the role of ectoine will be an issue for further investigations. Can ectoine production also be triggered by other stimuli than salinity? And what then happens in an ectoine-deficient mutant? The answer at least to the last question demands the availability of a genetic system that hopefully will be at hand soon.
Work by the authors' laboratory was supported by the German-Israeli Foundation for Scientific Research and Development (GIF). Volker Müller is indebted to former and present members of the "Müller salt group" for their hard work, creativity and never-ending enthusiasm.
- 1.Brinkmann R: Lehrbuch der allgemeinen Geologie. 1964, Stuttgart: Ferdinand Enke VerlagGoogle Scholar
- 2.Ott J: Meereskunde. 1996, Stuttgart: Ulmer Tachschenbuch Verlag, 2Google Scholar
- 7.Brown AD: Microbial water stress. Bacteriol Rev. 1976, 40: 803-846.Google Scholar
- 9.Sowers KR, Gunsalus RP: Halotolerance in Methanosarcina spp.: Role of Nε-acetyl-β-lysine, α-glutamate, glycine betaine, and K+ as compatible solutes for osmotic adaptation. Appl Environ Microbiol. 1995, 61: 4382-4388.Google Scholar
- 10.Cánovas D, Vargas C, Csonka LN, Ventosa A, Nieto JJ: Synthesis of glycine betaine from exogenous choline in the moderately halophilic bacterium Halomonas elongata. Appl Environ Microbiol. 1998, 64: 4095-4097.Google Scholar
- 15.Roberts MF, Lai MC, Gunsalus RP: Biosynthetic pathways of the osmolytes Nε-acetyl-β-lysine, β-glutamine, and betaine in Methanohalophilus strain FDF1 suggested by nuclear magnetic resonance analyses. J Bacteriol. 1992, 174: 6688-6693.Google Scholar
- 21.Ciulla RA, Burggraf S, Stetter KO, Roberts MF: Occurrence of di-myo -inositol-1-1'-phosphate in Methanococcus igneus. Appl Environ Microbiol. 1994, 176: 3177-3187.Google Scholar
- 22.Desmarais D, Jablonski PE, Fedarko NS, Roberts MF: 2-Sulfotrehalose, a novel osmolyte in haloalkaliphilic archaea. J Bacteriol. 1997, 179: 3146-3153.Google Scholar
- 23.Lanyi JK: Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriol Rev. 1974, 38: 272-290.Google Scholar
- 26.Oren A: The genera Haloanerobium, Halobacteroides and Sporohalobacter. The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications. Edited by: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer K-H. 1992, New York: Springer-Verlag, 1893-1900.Google Scholar
- 27.Oren A: Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev. 1999, 63: 334-348.Google Scholar
- 30.Duschl A, Wagner G: Primary and secondary chloride transport in Halobacterium halobium. J Bacteriol. 1986, 168: 548-552.Google Scholar
- 31.Schobert B, Lanyi JK: Halorhodopsin is a light-driven chloride pump. J Biol Chem. 1982, 257: 10306-10313.Google Scholar
- 38.Mongodin EF, Nelson KE, Daugherty S, Deboy RT, Wister J, Khouri H, Weidman J, Walsh DA, Papke RT, Sanchez Perez G, et al: The genome of Salinibacter ruber : convergence and gene exchange among hyperhalophilic bacteria and archaea. Proc Natl Acad Sci USA. 2005, 102: 18147-18152.CrossRefGoogle Scholar
- 44.Ferrer C, Mojica FJ, Juez G, Rodríguez-Valera F: Differentially transcribed regions of Haloferax volcanii genome depending on the medium salinity. J Bacteriol. 1996, 178: 309-313.Google Scholar
- 46.Spring S, Ludwig W, Marquez MC, Ventosa A, Schleifer K-H: Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacilus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol. 1996, 46: 492-496.CrossRefGoogle Scholar
- 47.Roeßler M, Müller V: Quantitative and physiological analyses of chloride dependence of growth of Halobacillus halophilus. Appl Environ Microbiol. 1998, 64: 3813-3817.Google Scholar
- 51.Drzewiecki K, Eymann C, Mittenhuber G, Hecker M: The yvyD gene of Bacillus subtilis is under dual control of σB and σH. J Bacteriol. 1998, 180: 6674-6680.Google Scholar
- 54.Müller V, Saum SH: The chloride regulon of Halobacillus halophilus : a novel regulatory network for salt perception and signal transduction in bacteria. Adaptation to life at high salt concentrations in Archaea, Bacteria, and Eukarya. Edited by: Gunde-Cimerman N, Oren A, Plemenitaš A. 2005, Dordrecht, The Netherlands: Springer, 9: 303-310.Google Scholar
- 56.Severin J: Kompatible Solute und Wachstumskinetik bei halophilen aeroben heterotrophen Eubakterien. Ph D thesis. 1993, University of Bonn, GermanyGoogle Scholar
- 58.Saum SH, Sydow JF, Palm P, Pfeiffer F, Oesterhelt D, Müller V: Biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium Halobacillus halophilus. J Bacteriol. 2006, 188: 6808-6815.CrossRefGoogle Scholar
- 59.Reitzer LJ: Ammonium assimilation and the biosynthesis of glutamine, glutamate, aspartate, asparagine, L-alanine, and D-alanine. Escherichia coli and Salmonella Cellular and Molecular Biology. Edited by: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. 1996, Washington, DC: Am. Soc. Microbiol, 1: 391-407.Google Scholar
- 64.Cánovas D, Borges C, Vargas A, Ventosa A, Nieto TT, Santos H: Role of Nγ-acetyldiaminobutyrate as an enzyme stabilizer an intermediate in the biosynthesis of hydroxyectoine. Appl Environ Microbiol. 1999, 65: 3774-3779.Google Scholar
- 65.Lippert K, Galinski AA: Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Appl Microbiol Biotechnol. 1992, 37: 61-65.Google Scholar
- 68.Pflüger K, Baumann S, Gottschalk G, Lin W, Santos H, Müller V: Lysine-2,3-aminomutase and β-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nε-acetyl-β-lysine and growth at high salinity. Appl Environ Microbiol. 2003, 69: 6047-6055.CrossRefGoogle Scholar
- 69.Madkour MA, Smith LT, Smith GM: Preferential osmolyte accumulation: a mechanism of osmotic stress adaptation in diazotrophic bacteria. Appl Environ Microbiol. 1990, 56: 2876-2881.Google Scholar
- 74.Csonka LN, Epstein W: Osmoregulation. Escherichia coli and Salmonella Cellular and Molecular Biology. Edited by: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE. 1996, Washington, DC: Am. Soc. Microbiol, 1: 1210-1223.Google Scholar
- 76.Wood JM: Osmosensing by bacteria: signals and membrane-based sensors. Microbiol Mol Biol Rev. 1999, 63: 230-262.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.