Malaria Journal

, 11:291 | Cite as

The baseline distribution of malaria in the initial phase of elimination in Sabang Municipality, Aceh Province, Indonesia

  • Puji BS Asih
  • Ismail E Rozi
  • Herdiana
  • Nandha R Pratama
  • Anggi PN Hidayati
  • Sylvia S Marantina
  • Sully Kosasih
  • Krisin Chand
  • Suradi Wangsamuda
  • Faisal A Rusdjy
  • Maria E Sumiwi
  • Ali Imran
  • Titik Yuniarti
  • Tahi Sianturi
  • Jamilah Nur
  • Asnita
  • Bukhari
  • Cut Barussanah
  • Muhammad Yani
  • Cut Ainun
  • Kurnia Jamil
  • Cut Mariam
  • Simon P Sengkerij
  • Ferdinand J Laihad
  • William Hawley
  • Din Syafruddin
Open Access
Research

Abstract

Background

Sabang Municipality, in Aceh Province, Indonesia, plans to initiate a malaria elimination programme in 2013. A baseline survey of the distribution of malaria in the municipality was conducted to lay the foundations for an evidence-based programme and to assess the island’s readiness to begin the elimination process.

Methods

The entire population of the municipality was screened for malaria infection and G6PD deficiency. Specimens collected included blood slides, blots and tubes for selected households.

Results and Discussion

Samples were collected from 16,229 residents. Microscopic examination of the blood smears revealed 12 malaria infections; 10 with Plasmodium falciparum and 2 with Plasmodium vivax. To confirm the parasite prevalence, polymerase chain reaction (PCR) diagnosis was performed on the entire positive cases by microscopy and randomized 10% of the microscopically negative blood samples. PCR revealed an additional 11 subjects with malaria; one P. falciparum infection from the village of Paya Keunekai, and nine P. vivax infections and one mixed P. falciparum/P. vivax infection from the village of Batee Shok. The overall slide positivity rate was 0.074% (CI 95%: 0.070 – 0.078) and PCR corrected prevalence 0,590% (CI 95%: 0.582 – 0.597). Analysis of 937 blood samples for G6PD deficiency revealed two subjects (0.2%) of deficient G6PD. Analysis of several genes of the parasite, such as Pfdhfr, Pfdhps, Pfmdr1, Pfcrt, Pfmsp1, Pfmsp2, Pvdhfr, Pvdhps, Pvmdr1 and host gene, such as G6PD gene revealed that both P. falciparum and P. vivax carried the mutation associated with chloroquine resistance.

Conclusion

Malariometric and host genetic analysis indicated that there is a low prevalence of both malaria and G6PD deficiency in the population of Sabang Municipality. Nevertheless, malaria cases were clustered in three rural villages and efforts for malaria elimination in Sabang should be particularly focused on those three villages.

Keywords

Malaria Mass Drug Administration G6PD Deficiency Falciparum Isolate Vivax Isolate 

Background

Malaria remains a major public health problem in Indonesia, with 30 million cases and 120,000 deaths annually. Recently measures of annual parasite incidence (API) have varied substantially between provinces, but the highest API is consistently detected in the eastern parts of Indonesia. Aceh Province, Sumatera, is the western most point of the archipelago, and malaria has been documented throughout. However, there have been few published reports of malaria prevalence in Sumatera in general and in Aceh in particular. Malaria is endemic in 21 districts and municipalities in Aceh Province, where the annual malaria incidences (AMI) in 2003 and 2004 were 4.94% and 3.2% respectively[1]. Malaria prevalence surveys in 2005 and 2006 in 11,763 subjects from 3,771 households in five districts along the tsunami-affected western coastline reported a slide positivity rate for all Plasmodium species of 2.1%[2].

Malaria in Sabang has been documented by several previous surveys both before and after the 2004 tsunami that destroyed much of the coastal region in Aceh[1]. Resistance to chloroquine and sulphadoxine-pyrimethamine, two historical anti-malarial mainstays, was reported in an in vitro study conducted during 1984–1985[3], findings which were supported by an in vivo study in 2004[4]. The AMI of Sabang Municipality fell from 269 cases per 1,000 populations in 2001 to 33 cases per 1,000 populations in 2009, while the API similarly fell from 101 cases in 2001 to 3 cases per 1,000 populations in 2009[3]. Malariometric surveys in the villages of Suka Jaya and Paya Seunara found that the slide positivity rates decreased from 7.4% in 2005 to 5.4% in 2006. Measurement of the prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency using a colorimetric assay identified 0.8% subjects with less G6PD activity, and molecular analyses found Vianchang type G6PD deficiency mutation (Syafruddin, unpublished report).

The Indonesian Ministry of Health announced a country wide malaria elimination policy in 2009 that envisioned a combat against the disease lasting from 2010 to 2030. Aceh Province aims to eliminate malaria by 2015 but for Sabang Municipality, which includes the islands of Weh, Rondo and Rubiah, the target for malaria elimination is 2013[5]. An optimal strategy for the elimination programme will be based on a contemporary reassessment of the malaria situation as well as the G6PD prevalence in the islands. Appropriate knowledge on G6PD prevalence is important, haemolysis may be induced during primaquine treatment among G6PD-deficient individuals. To accomplish this, a study was conducted in Sabang Municipality to obtain baseline data on malaria prevalence as well as host genetic factors, such as G6PD deficiency, that may contribute to malaria morbidity.

Methods

Study site

Sabang Municipality is administratively included in Aceh Province, Indonesia. It is located at latitude 5° 49' 30" north and longitude 95° 18' 28" east (Figure1). It occupies an area of 4,051 sq km, and in 2009 its total population was 24,815. The rainy season usually occurs from December to April, during which time the temperature centres around 18–20°C. Dry season temperatures are around 25-33°C.
Figure 1

Aceh Province with Sabang Municipality indicated by the arrow and location within the Indonesian archipelago (inset). Not to scale.

Malariometric and G6PD surveys

Malariometric and G6PD surveys were conducted from May to October 2010, in 14 villages with known malaria transmission. Urban areas with no recorded transmission were excluded from the survey. Malariometric surveys include interview, physical examination of malaria symptoms and signs, body weight and axillary temperature. For each individual, thick and thin blood smears were collected along with a blood spot for PCR on filter paper and whole blood drops for G6PD test and haemoglobin (Hb) tests. All slides were reviewed by an expert microscopist. Slide positive cases were confirmed with PCR. In addition, 10% of negative cases were screened with PCR, and all blood samples from villages with microscopically positive cases. Selection of the 10% negative samples was done through computer programme. All negative samples in each village were listed sequentially in microsoft excel. A Random option was used to generate sample sequence and the samples were selected in ten-fold fashion. At the end, 1,725 samples were proportionally selected. Locations of confirmed positive cases were geocoded with GPS (Figure2). For G6PD test, blood were directly dropped onto a 1.5 ml microtube containing 760 μl of water, 20ul of substrate mixture and 20 μl of dye mixture of G6PD assay kit. The tube was then vigorously shaked for 5 sec and incubated at 25-37°C for 20–30 minutes. The reaction was stopped by adding 10 μl of 1 mol/l HCL and compared the developed color with those of positive and negative controls. This method used water-soluble tetrazolium salt, WST-8, that also produces water-soluble formazan (Dojindo Laboratories, Tokyo, Japan). The G6PD enzyme activity was divided into five categories: A: 0% activity (G6PD-deficient), B: 25% activity, C: 50% activity, D: 75% activity and E: 100% activity (normal activity). The sample selection for G6PD screening was based on households in urban village of Cot Bau where the population of the entire municipality was well represented. In each selected household, chief of the household and his wife were enrolled. The total subjects enrolled in G6PD survey was 937 subjects and represented over 50% of the total households in this village. To determine the Hb status, a portable digital haemocytometer (Haemocue AB Hb201+, Angelholm, Sweden) was used.
Figure 2

Malaria case (★) distribution and Primary Health Center (+) in Sabang Municipality. Not to scale.

Laboratory methods

Thick and thin blood films were stained with Giemsa and examined using 1,000X oil immersion light microscopy. At least 200 ocular fields were examined before considering a slide negative, and parasite densities were counted as parasites per 200 leukocytes and reported as parasites/mm3 assuming a white blood cell count of 8,000/mm3[6].

Extraction of DNA

Parasite and human host DNA was extracted from blood samples using chelex-100 ion exchange (Biorad Laboratories, Hercules, CA, USA) according to a procedure described previously[7]. The DNA was either used immediately for PCR amplification or stored at −20°C for later analysis.

Molecular analyses on the parasite and host

Molecular analyses were performed using PCR amplification, restriction fragment length polymorphisms (RFLP), and sequencing on several genes of the parasite such as Pfdhfr, Pfdhps, pfmdr1, pfcrt[8, 9, 10], Pfmsp1, Pfmsp2[11, 12], Pvdhfr, Pvdhps[13, 14], Pvmdr1[15] and host gene such as G6PD gene[16]. The PCR reactions were carried out in a condition similar to the previously published report[8, 9, 10, 11, 12, 13, 14, 15, 16]. PCR diagnosis to further confirm the parasite rate on the entire positive cases was performed using 18S rRNA gene primer in a condition similar to the previously published report[17].

Results

Malariometric surveys

In all, 16,229 individuals were surveyed from 14 villages. The surveys covered 83.47% of the total inhabitants of the targeted survey areas and by microscopy 10 subjects were found with Plasmodium falciparum infection: seven from the village of Batee Shok, two from Paya Keunekai and one from Ie Meuleu. Plasmodium vivax malaria was found in one subject from Iboih and one from Rondo island. PCR testing was performed on seven malaria falciparum slide positive cases and one malaria vivax slide positive case (blood blots were unavailable for three malaria falciparum slide positive cases and one malaria vivax slide positive case). PCR (10% of negative cases and all blood samples from villages with microscopically positive) revealed an additional 11 malaria-positive subjects: one mixed P. falciparum/P. vivax infection from Batee Shok, one P. falciparum from Paya Keunekai village, and nine P. vivax from Batee Shok village (Table1 and2). The slide positive rate was 0.074% (CI 95%: 0.070 – 0.078) and PCR corrected 0.590% (CI 95%: 0.582 – 0.597). Of those that were positive for malaria by microscopy, 9 (75%) had clinical symptoms such as fever and/or history of headache. The remaining 14 subjects, all from the village of Batee Shok, had neither symptoms nor history of recent fever. Overall, of the total 23 positive cases by microscopy and PCR, 17 (73.9%) cases were found at Batee Shok and the remainder were found in Iboi, Paya Keunekai, Ie Meulee and Rondo island. However, malaria prevalence peaked in the village of Batee Shok, which harboured 17 malaria-positive subjects among a total 1,109 subjects examined (1.53%).
Table 1

Malaria positives by village in Sabang Municipality

 

Detection method

 

Location in Sabang

Microscopy

 

PCR*

Total

 

Pf

Pv

 

Pf

Pv

 

Iboih

-

1

 

-

-

1

Batee Shok

7

-

 

1

9

17

Paya Keunekai

2

-

 

1

-

3

Ie Meulee

1

-

 

-

-

1

Rondo Island

-

1

 

-

-

1

Total

10

2

 

2

9

23

* = 10% of negative cases and all blood samples from villages with microscopically positive.

Table 2

Number of PCR positives samples from different groups

No

Sample sources

Total PCR

PCR Results

Pf

Pv

Mixed

1

Microscopy positive subjects

8*

10

2

0

2

Villages with microscopy positive

    

Iboih

284

0

0

0

Batee Shok

898

0

9

1

Paya Keunekai

308

0

1

0

3

10% random sample microscopy negative from cencus data in each village

1,725

0

0

0

 

Total

3,223

10

12

1

Total PCR corrected prevalence = 0.590% (19/3223) (CI 95%: 0.582 – 0.597).

Total microscopy prevalence = 0.074% (12/16229) (CI 95%: 0.070 – 0.078).

(*) 4 of 12 samples have no blood blots.

Prevalence of G6PD deficiency

The head of each household and his wife were enrolled in the G6PD survey. Of the total 937 subjects enrolled, two (0.2%) tested positive for G6PD deficiency. Analysis for the presence of the most commonly found mutations: Mediterranean (563C/T), Coimbra (592 C/T), Vianchang Canton (1376 G/T), Vanua Lava (383 T/C), Mahidol (487 G/T), Chatam (1003 G/A), Kaiping (1388 G/A) and Vianchang (871 G/A, 1311 C/T) found none of these in the two positive subjects.

Haemoglobin status

A total of 16,229 subjects was analysed for weight and Hb levels. The mean of Hb level was relatively high in each age group except for the below five years, which had a Hb level slightly above or below the borderline (anaemia cut-off for Hb level is 11 g/dL). Distribution of age, children, sex and mean of Hb level per village also indicated relatively high in each group. However, due to the low prevalence of malaria, the relationship between anaemia and malaria could not be determined. Malnutrition was found in one village Kreung Raya (Additional files1,2,3,4, and5).

Genotypic profiles of Plasmodium falciparum isolates

Molecular analysis of the parasites collected throughout the survey found the 76 T allele of the pfcrt gene, a molecular marker for parasite resistance to chloroquine (Table3). The proportion of the isolates carrying the 86Y polymorphism of the pfmdr1 gene was 37.5%. The 1042D polymorphism of pfmdr1 was detected in one isolate. No polymorphisms at codons 1032 and 1246 of the pfmdr 1 gene were observed in any of the isolates examined. Amplification of the dhfr gene indicated that the majority of the isolates carried the mutant 108 N allele. 108T, 59R, 16V, 50R, 51I, 164L, 436A 437G, 540E, 581G and 613 S/T polymorphisms were not detected in any of the isolates examined. Analysis of the parasite genotype indicated infection with multiple parasite strains (Table3). All samples carried the 3D7 type of which three samples were mixed with the FC27 type. Analysis on MSP1 gene revealed the K1, MAD20 and RO33 types but three samples could not be determined.
Table 3

Genotypic profile of Plasmodium falciparum

Gene analysed

Sample Code

PfMSP2

PfMSP1

PfCRT

PfMDR1

PfDHFR

PfDHPS

 

3D7

FC27

KI

MAD20

RO33

K76T

N86Y

N1042D

A16V

C59R

S108N/T

A437G

K540E

ASK10057

+

--

+

+

--

T

Y

N

A

C

N

A

K

ASK20503

+

+

--

--

--

T

N

N

A

C

S/N

A

K

ASK20505

+

--

+

--

--

T

N

N

A

C

N

A

K

ASK20512

+

--

--

--

--

T

Y

N

A

C

N

A

K

ASK20535

+

--

--

--

--

T

N

N

A

C

S

A

K

ASJ10083

+

+

--

+

--

T

Y

N

A

C

N

A

K

ASJA0324

+

--

--

+

--

T

N

N

A

C

N

A

K

MC-HN

+

+

+

--

+

T

N

N/D

A

C

S/N

A

K

No blood blots for three samples.

Genotypic profiles of Plasmodium vivax isolates

Molecular analysis of the parasites collected throughout the survey indicated that the 976 F allele of the pvmdr1 gene, a molecular marker for resistance to chloroquine, was found in 10 of 11 isolates examined. Amplification of the Pvdhfr gene indicated that 63.6% of the isolates carried the wild type 117S allele and the reminder carried the 117 T. Mixed allelic infection of 117 T/N was found in one isolate. Analysis of codon 57 and 58 of the Pvdhfr gene revealed three isolates carried the 57 F allele and five isolates carried the 58S allele, three of which are mixed allelic infection with 58R. No polymorphisms were observed at codons 13 or 16 in any of the isolates examined. Furthermore, no polymorphisms were observed at codons 383 and 553 of Pvdhps gene in any of the isolates examined in this study (Table4).
Table 4

Genotypic profile of Plasmodium vivax

No Sample

Pvmdr1

PvDHFR

PvDHPS

Y976F

S117T/N

F57I/L

I13L

T61M

S58R

A383G

A553G

ASK20097

F

S

L

I

M

S

G

A

ASK20143

F

S

L

I

M

R

G

A

ASK20260

F

T

L

I

M

R

G

A

ASK20328

F

S

L

I

M

R

G

A

ASK20334

F

S

L

I

M

R

G

A

ASK20483

F

T

L

I

M

S

G

A

ASK20712

F

S

L

I

M

S + R

G

A

ASK20747

F

S

F

I

M

R

G

A

ASK20803

F

T + N

F

I

M

S + R

G

A

ASK20953

F

T

L

I

M

S + R

G

A

ASJ10148

Y

S

F

I

M

R

G

A

No blood blots for one sample.

Discussion

Mass blood surveys conducted in three periods between May and October 2010 describe a low prevalence of malaria in Sabang Municipality. P. falciparum and P. vivax were found, with P. falciparum predominant. The findings that most of the malaria-positive subjects had clinical symptoms suggest that malaria endemicity in the island is relatively unstable. Nevertheless, the relatively high proportion of asymptomatic, sub-patent (microscopy negative and PCR positive) infection alerts to the need of a more reliable detection method as the cases might be potential source of infection as that found in South America[18]. Malaria cases were found in only three rural villages, corroborating previous observations[4]. It is highly recommended that efforts be concentrated on containing malaria in this village through active and passive case-finding and prompt, appropriate treatment.

Genotyping found that all of the P. falciparum isolates examined carried chloroquine-resistant alleles. All of the isolates examined carried the 76 T mutant allele of the pfcrt gene and in addition 30% of isolates carried the 86Y allele of the pfmdr1 gene. This result is in line with previous observations that the parasite on this island is resistant to chloroquine[1]. In addition, among the P. vivax isolates examined, 10 carried the 976 F allele of the pvmdr1 gene that has been associated with chloroquine resistance[9, 11]. Both P. falciparum and P. vivax in this island are resistant to chloroquine, providing a rational basis for the use of arteminisin-based combination therapy for any malaria cases found.

Analysis of the Pfdhfr gene also indicated a high proportion of the P. falciparum isolates carry mutant alleles associated with resistance to pyrimethamine. However, the frequency with which the mutant allele of Pfdhfr gene was found is slightly lower than that of a previous study done in 2006, likely due to the larger sample size of our study. Pfdhps alleles associated with sulphadoxine resistance were not found in any of the isolates examined, indicating that the parasites on this island are still sensitive to treatment with sulphadoxine-pyrimethamine, quintuple mutations in dhfr and dhps being associated with sulphadoxine-pyrimethamine treatment failure[12].

The prevalence of G6PD-deficiency cases was also low (0.2%) among the 937 subjects screened. These subjects represent households and the prevalence reflects a larger population, but the G6PD deficiency prevalence found in this survey is much lower than that found in a previous survey (0.8%) that was conducted 2006 (unpublished observation). The difference might be attributed to the much higher number of subjects involved in this study. In the previous G6PD survey, the Vianchang mutation (871 G/A, 1311 C/T) was found in one subject but during the current survey none of the most common G6PD mutations were found among the G6PD-deficient cases.

Conclusions

This combined malariometric and host genetic analysis indicated that both malaria and G6PD deficiency exist in low prevalence among the examined population in Sabang Municipality. In the context of the malaria elimination programme, several rational actions are implied; i) more flexible use of primaquine, the only available anti-malarial for antigametocyte and radical cures; ii) to contain malaria transmission in this island, a limited mass drug administration could be performed at the three villages where the malaria cases was detected; and iii) active case monitoring and treatment on migrants should be prepared.

Financial support

This study was funded by UNICEF Indonesia.

Notes

Acknowledgements

The authors are grateful for the support of the Eijkman Institute Jakarta, UNICEF Indonesia and Department of Health, Republic of Indonesia. The authors wish to thank Dian Sidiq Arsyad, M Kes from University of Hasanuddin for his help in statistical analysis, Michael Kinzer MD, CDC USA for critical reading of the manuscript, all colleagues from University of Syiah Kuala, professional staff at Aceh Provincial Health Department, professional staff at Provincial Health Laboratory (Balai Laboratorium Kesehatan Aceh), professional staff at Department of Health Sabang Municipality, and Puskesmas at Sabang Municipality for their assistance during sample collection, also to Sabang Mayoralty Officer and the Sabang Mayoralty People Council (DPRK) and people from Sabang Mayoralty for their cooperation and support during the surveys.

Supplementary material

12936_2012_2425_MOESM1_ESM.doc (55 kb)
Additional file 1: Detailed number of MBS subjects and coverage percentage of enrollment.(DOC 55 KB)
12936_2012_2425_MOESM2_ESM.doc (36 kb)
Additional file 2: Distribution of respondents by age group.(DOC 36 KB)
12936_2012_2425_MOESM3_ESM.doc (50 kb)
Additional file 3: Descriptive Statistic for Hb level by village.(DOC 50 KB)
12936_2012_2425_MOESM4_ESM.doc (51 kb)
Additional file 4: Distribution of anaemic status by village.(DOC 51 KB)
12936_2012_2425_MOESM5_ESM.doc (36 kb)
Additional file 5: Age distribution of participants in MBS divided per villages.(DOC 36 KB)
12936_2012_2425_MOESM6_ESM.png (245 kb)
Authors’ original file for figure 1
12936_2012_2425_MOESM7_ESM.png (362 kb)
Authors’ original file for figure 2

References

  1. 1.
    Ministry of health, Indonesia: Buletin Penelitian Kesehatan. 1984,  , JakartaGoogle Scholar
  2. 2.
    Muriuki D, Hahn S, Hexom B, Allan R: Cross-sectional survey of malaria prevalence in tsunami-affected districts of Aceh Province, Indonesia. Int J Emergency Med. 2012, 5: 11-10.1186/1865-1380-5-11.CrossRefGoogle Scholar
  3. 3.
    Tjitra E, Gunawan S, Laihad F, Sulaksono S, Arjoso S, Manurung N: Evaluation of antimalarial drugs in Indonesia 1981–1995. Buletin Penelitian Kesehatan. 1997, 25: 27-58.Google Scholar
  4. 4.
    Maryatun: Evaluation of chloroquine treatment failure among of uncomplicated malaria falciparum and its contributing factors in Sabang, Nanggoe Aceh Darussalam Province, (Penilaian kasus kegagalan pengobatan klorokuin terhadap penderita malaria falciparum dan faktor-faktor yang mempengaruhinya (kajian secara in vivo pada penderita malaria falciparum ringan/tanpa komplikasi di Kota Sabang. 2004, Gajah Mada University, Yogyakarta, IndonesiaGoogle Scholar
  5. 5.
    Ministry of Health 2009: Regarding malaria elimination in Indonesia. 2009, Minister Decree No. 293/MENKES/SK/IV/2009, JakartaGoogle Scholar
  6. 6.
    Gilles HM: Diagnostic method in malaria. Bruce-Chwatt’s Essential Malariology. Edited by: Gilles HM, Warrel DA. 1993, Oxford University press, New York, 79-95.Google Scholar
  7. 7.
    Wooden J, Kyes S, Sibley CH: PCR and strain identification in Plasmodium falciparum. Parasitol Today. 1993, 9: 303-305. 10.1016/0169-4758(93)90131-X.CrossRefPubMedGoogle Scholar
  8. 8.
    Duraisingh MT, Curtis J, Warhurst DC: Plasmodium falciparum: detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion. Exp Parasitol. 1998, 89: 1-8. 10.1006/expr.1998.4274.CrossRefPubMedGoogle Scholar
  9. 9.
    Babiker HA, Pringle SJ, Abdel-Muchsin A, Mackinnon M, Hunt P, Walliker D: High- level chloroquine resistance in Sudanese isolates of P. falciparum is associated with mutations in the chloroquine resistance transporter gene, pfcrt and the multidrug resistance gene pfmdr1. J Infect Dis. 2001, 183: 1535-1538. 10.1086/320195.CrossRefPubMedGoogle Scholar
  10. 10.
    Syafruddin D, Asih PBS, Casey G, Maquire J, Baird JK, Nagesha HS, Cowman AF, Reeder J: Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia. AmJTrop Med Hyg. 2005, 72: 174-181.Google Scholar
  11. 11.
    Snounou G, Beck HP: The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol Today. 1998, 14: 462-467. 10.1016/S0169-4758(98)01340-4.CrossRefPubMedGoogle Scholar
  12. 12.
    Snounou G, Färnert A: Genotyping of Plasmodium falciparum parasites. 2008, Methods in malaria research, 238-242.Google Scholar
  13. 13.
    Hawkins VN, Auliff A, Prajapati SK, Rungsihirunrat K, Hapuarachchi HC, Maestre A, TO'Neil M, Cheng Q, Joshi H, NaBangchang K, Sibley CH: Multiple origins of resistance-conferring mutations in Plasmodium vivax dihydrofolate reductase. Malar J. 2008, 7: 72-10.1186/1475-2875-7-72.PubMedCentralCrossRefPubMedGoogle Scholar
  14. 14.
    Hawkins VN, Joshi H, Rungsihirunrat K, Na-Bangchang K, Sibley CH: Antifolates can have a role in the treatment of Plasmodium vivax. Trends Parasitol. 2007, 23: 5-10.1016/j.pt.2006.11.002.CrossRefGoogle Scholar
  15. 15.
    Brega S, Meslin B, de Monbrison F, Severini C, Gradoni L, Udomsangpetch R, Sutanto I, Peyron F, Picot S: Identification of the Plasmodium vivax mdr-like gene (pvmdr1) and analysis of single-nucleotide polymorphisms among isolates from different areas of endemicity. J Infect Dis. 2005, 191: 272-277. 10.1086/426830.CrossRefPubMedGoogle Scholar
  16. 16.
    Matsuoka H, Vinh Thuan DT, Thien H, Kanbe T, Jalloh A, Hirai M, Arai M, Dung NT: Kawamoto: Seven different glucose-6-phospate dehydrogenase variants including a new variant distributed in Lam Dong Province in Southern Vietnam. Acta Med Okayama. 2007, 61: 213-219.PubMedGoogle Scholar
  17. 17.
    Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA: A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. AmJTrop Med Hyg. 1999, 60: 687-692.Google Scholar
  18. 18.
    Coura JR, Suarez-Mutiz M: A new challenge for malaria control in Brazil: asymptomatic Plasmodium infection – A Review. Mem Ins Oswaldo Cruz. 2006, 101: 229-237.Google Scholar

Copyright information

© Asih et al.; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Puji BS Asih
    • 1
  • Ismail E Rozi
    • 1
  • Herdiana
    • 2
  • Nandha R Pratama
    • 1
  • Anggi PN Hidayati
    • 1
  • Sylvia S Marantina
    • 1
  • Sully Kosasih
    • 1
  • Krisin Chand
    • 1
  • Suradi Wangsamuda
    • 1
  • Faisal A Rusdjy
    • 1
  • Maria E Sumiwi
    • 1
  • Ali Imran
    • 3
  • Titik Yuniarti
    • 3
  • Tahi Sianturi
    • 3
  • Jamilah Nur
    • 3
  • Asnita
    • 3
  • Bukhari
    • 3
  • Cut Barussanah
    • 4
  • Muhammad Yani
    • 4
  • Cut Ainun
    • 5
  • Kurnia Jamil
    • 5
  • Cut Mariam
    • 6
  • Simon P Sengkerij
    • 2
  • Ferdinand J Laihad
    • 2
  • William Hawley
    • 2
  • Din Syafruddin
    • 1
    • 7
  1. 1.Eijkman Institute for Molecular BiologyJakartaIndonesia
  2. 2.UNICEFJakartaIndonesia
  3. 3.Office Health DepartmentSabang MunicipalityAceh ProvinceIndonesia
  4. 4.Provincial Health LaboratoryAceh ProvinceIndonesia
  5. 5.Department of Internal Medicine, Faculty of MedicineUniversity of Syah KualaSyah KualaIndonesia
  6. 6.Department of Parasitology, Faculty of MedicineUniversity of Syah KualaSyah KualaIndonesia
  7. 7.Department of Parasitology, Faculty of MedicineHasanuddin UniversityMakassarIndonesia

Personalised recommendations