Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac
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The signaling cascades that direct the morphological differentiation of the vascular system during early embryogenesis are not well defined. Several signaling pathways, including Notch and VEGF signaling, are critical for the formation of the vasculature in the mouse. To further understand the role of Notch signaling during endothelial differentiation and the genes regulated by this pathway, both loss-of-function and gain-of-function approaches were analyzed in vivo.
Conditional transgenic models were used to expand and ablate Notch signaling in the early embryonic endothelium. Embryos with activated Notch1 signaling in the vasculature displayed a variety of defects, and died soon after E10.5. Most notably, the extraembryonic vasculature of the yolk sac displayed remodeling differentiation defects, with greatly enlarged lumens. These phenotypes were distinct from endothelial loss-of-function of RBPJ, a transcriptional regulator of Notch activity. Gene expression analysis of RNA isolated from the yolk sac endothelia of transgenic embryos indicated aberrant expression in a variety of genes in these models. In particular, a variety of secreted factors, including VEGF and TGF-β family members, displayed coordinate expression defects in the loss-of-function and gain-of-function models.
Morphological analyses of the in vivo models confirm and expand the understanding of Notch signaling in directing endothelial development, specifically in the regulation of vessel diameter in the intra- and extraembryonic vasculature. Expression analysis of these in vivo models suggests that the vascular differentiation defects may be due to the regulation of key genes through the Notch-RBPJ signaling axis. A number of these genes regulated by Notch signaling encode secreted factors, suggesting that Notch signaling may mediate remodeling and vessel diameter in the extraembryonic yolk sac via autocrine and paracrine cell communication. We propose a role for Notch signaling in elaborating the microenvironment of the nascent arteriole, suggesting novel regulatory connections between Notch signaling and other signaling pathways during endothelial differentiation.
KeywordsNotch Signaling Dorsal Aorta Vascular Endothelial Growth Factor Signaling Endothelial Differentiation Vascular Endothelial Growth Factor Family Member
The formation of the vascular system is essential for nutrient and waste transport in the growing embryo. In mice, the developing vasculature initially forms in intraembryonic and extraembryonic regions. In the extraembryonic yolk sac at approximately E7.0-7.5, angioblasts are formed from the differentiation of mesodermal cells. These angioblasts differentiate into endothelial cells, elaborate cell contacts, and lumenize into simple tubes; resulting in the formation of a capillary plexus network [1, 2]. The simple plexus of the yolk sac is remodeled and refined after E8.5 to form the larger diameter vessels. During this process, extensive movements of endothelial cells within the plexus occur through a process termed intussusceptive arborization , reallocating cells from the capillaries to larger vessels, to assemble a more complex vasculature network [4, 5]. This process forms the vitelline arteriole and venule, which participate in the contiguous blood flow with the embryonic vasculature, concomitant with the initiation of flow after E9.0. Although likely context dependent, vessel remodeling also occurs in the adult, during wound healing, reproductive cycling, and tumor progression . More work needs to be done to define the shared and distinct regulatory paths that control vascular differentiation in the various sites of development and in the adult.
Both vasculogenic and angiogenic processes are highly regulative, and under the control of a number of signaling pathways, including the vascular endothelial growth factor (VEGF) pathway, the Notch pathway, and the transforming growth factor-β (TGF-β) pathway, among others [7, 8, 9, 10]. Notch signaling is an evolutionary conserved pathway and a determinant of cell fate . Four Notch receptors (Notch 1-4) exist in mice and human along with five ligands (Jagged1 and -2, and Dll1, -3, and -4) . The Notch receptors are activated upon ligand binding, which initiates the proteolysis of its intracellular domain (N-ICD). The N-ICD translocates to the nucleus where it interacts with a family of DNA-binding proteins, termed recombination signal-binding protein for immunoglobulin kappa J region (RBPJ; also known as C-promoter binding factor 1, CBF1), forming a transcriptional activator complex at the regulatory elements of target genes, thereby directing changes in gene expression transcription .
Much work has been done to define the roles of the Notch signaling pathway during vascular differentiation. Notch1, Notch4, Dll4, Jagged1, and Jagged2 are all expressed in the arterial endothelium of vertebrates, Notch4 being solely expressed in the endothelia of mouse embryos [13, 14]. Mutations in these genes lead to defects in the vasculature, many of which are embryonic lethal. Mutant mice lacking Notch1 do not survive post E11.5 and harbor defects in vascular remodeling in the embryo, yolk sac, and placenta . Deletion of Notch4 has no visible effect and embryos are viable; however, Notch1 -/- Notch4 -/- double mutants have more severe vascular phenotypes than the Notch1 -/- and are embryonic lethal at E9.5 [10, 16]. Expression of an activated form of Notch4 or Notch1 also leads to vascular defects similar to those seen in the Notch1 -/- and Notch1 -/- Notch4 -/- mice, as well as embryonic lethality at ~E10 [17, 18].
Although Notch clearly plays important roles in the formation of the early embryonic vasculature, very little is known about the nature of the downstream targets in vivo, and how changes in Notch activity elicit the observed morphological processes. In vitro analysis has indicated novel Notch targets, including receptors of the VEGF family, VEGFR-3 (Flt4) and VEGFR-1 (Flt1) [19, 20]. Given that several signaling cascades are required for the morphological differentiation of the embryonic vasculature, it is likely that these pathways interact during vascular development.
To better define the activity of Notch signaling in vascular differentiation, a detailed morphological and molecular analysis was performed using developmental models in which the Notch signaling pathway is altered. A gain-of-function Notch1 transgenic model showed that expanded Notch1 signaling in the early vasculature results in defects in embryo growth, defective differentiation during remodeling of the yolk sac vasculature, altered patterns of gene expression, and ultimately embryonic lethality. These phenotypes were compared to embryos lacking Notch signaling in the endothelia, via a tissue-specific loss of RBPJ function. Embryos lacking endothelial Rbpj exhibited distinct growth, vascular, and gene expression defects compared to the Notch1 gain-of-function model. Gene expression analysis in the yolk sacs of these models demonstrated altered patterns of expression of a distinct subset of Notch targets. Additionally, several secreted ligands, including the TGF-β ligand TGFβ2 and the VEGF ligands VEGFC and Placenta Growth Factor (PlGF), were altered in these models, suggesting a role for an altered VEGF signaling pathway in the observed phenotypes of these models. Our data suggest a model in which Notch signaling in the endothelia is critical for elaborating a specialized local environment of the developing arterial vasculature, by influencing the expression of secreted factors, which may be important in autocrine or paracrine signaling to direct further morphological differentiation of the vasculature during remodeling.
Conditional transgenesis to modulate Notch signaling in the early endothelia
Tie2-Cre  or Flk1-Cre  transgenic mice were crossed to either Rosa Notch or Rbpj f mice. Each of these transgenes expresses the Cre recombinase gene and direct expression principally to the vascular endothelium. Identical phenotypes were observed when the Flk1-Cre and Tie2-Cre transgenic lines were crossed to the Rosa Notch and Rbpj f models (data not shown). Previous work has demonstrated that these Cre expressing transgenes exhibit restricted endothelial expression of Cre recombinase activity to the endothelial and hematopoietic lineages . The early embryonic expression and recombinase activity of the Tie2-Cre and Flk1-Cre transgenes was confirmed by crossing these transgenes to a conditional LacZ reporter . Both transgenes showed specific expression within the early endothelial and hematopoietic lineages at E8.5 (Additional File 1).
Regulated Notch signaling is essential for the growth and development of the early embryo
To ablate Notch signaling in the embryo, Tie2-Cre mice were used in a two-generation cross to generate Tie2-Cre; Rbpj f/f embryos (EC-Rbpj-KO), which lack RBPJ binding activity in the endothelia. The EC-Rbpj-KO embryos displayed severe growth retardation defects at E9.5 similar to those observed in the EC-N1ICD embryos (Figure 2C). The morphological analyses of the gain-of-function and loss-of-function embryos were consistent with other models [10, 17, 18, 26] and confirmed that the appropriate Notch signaling in the endothelia of the early embryo is critical for proper growth and development of the embryo.
Vascular defects in EC-N1ICD and EC-Rbpj-KO embryos
A detailed comparison of the vasculature was performed to define the vascular defects in the embryonic and extraembryonic vasculature of EC-N1ICD and EC-Rbpj-KO embryos. At E8.5, no overt defects were observed in the developing vasculature of either the EC-N1ICD or EC-Rbpj-KO. In particular, the vascular plexus of the EC-N1ICD yolk sac, visualized by histochemical staining of endothelial cells with an antibody to PECAM1 (CD31), appeared unaffected when compared to stage-matched wild type embryos (Additional File 2; data not shown). The most severe defects were seen in the yolk sac of the developing embryo beginning at approximately E9.5. Gross morphological examination of the vasculature of the yolk sac by whole mount light microscopy showed that at E9.5 the EC-N1ICD yolk sac was the same size as the stage-matched wild type control; however these embryos lacked large diameter vessels (Figure 2D,E), indicating a failure in appropriate blood vessel remodeling occurring at this time point . The EC-N1ICD yolk sac vessels did contain blood cells, although the blood tended to pool near the proximal end of the yolk sac adjacent to the chorioallantoic plate (Figure 2E). EC-Rbpj-KO embryos also lacked vascular remodeling in the yolk sac, failing to form the large vitelline blood vessels  (Figure 2F).
Immunofluorescence of PECAM1 stained E9.5 yolk sac revealed the failure of this remodeling in both the EC-N1ICD and EC-Rbpj-KO embryos in greater detail. In the EC-N1ICD embryos, no distinction between large caliber vessels and capillaries was observed (Figure 2H); instead, the vasculature consisted of vessels with an enlarged surface area with greatly decreased avascular inter-vessel space compared to wild type controls, as assessed by lack of PECAM1 expression ('pillars'; Figure 2G,H, arrows). In contrast to the EC-N1ICD vessel defects, the EC-Rbpj-KO embryos exhibited a qualitatively different vessel phenotype in the yolk sac. Although EC-Rbpj-KO embryos also exhibited a lack of vascular remodeling, the yolk sac of these embryos displayed numerous small avascular intervessel spaces (Figure 2I, arrow). These results indicated that the yolk sac vasculature of the EC-Rbpj-KO embryos failed to form the large vitelline blood vessels, reminiscent of the simple vascular plexus seen at E8.5.
The morphological analyses of the models of altered Notch signaling were consistent with previous analysis of Notch function in the vasculature [10, 17, 18, 26]. These results point to multiple roles for Notch signaling in the formation of both the intra- and extraembryonic vasculature, including the remodeling of the yolk sac vasculature, the regulation of vessel diameter, and the invasion of the fetal vasculature into the labyrinth layer of the placenta.
Identification of Notch regulated genes in EC-N1ICD yolk sac and EC-Rbpj-KO yolk sac
The members of the Hairy/Enhancer of split-related (HES/HEY) family are well described Notch targets, many of which are directly regulated by Notch1 signaling via its binding to RBPJ  to regulatory regions of these loci. The microarray datasets revealed that in yolk sacs of EC-N1ICD embryos, both Hey1 and Heyl were highly induced, and expression of these genes was reduced in the EC-Rbpj-KO; these results were confirmed by semi-quantitative RT-PCR (Figure 4A). Expression of Hey1 and Heyl was increased 4.4-fold and 20.5-fold in the EC-N1ICD model respectively, and decreased to 35% and 46% of wild type levels in the EC-Rbpj-KO yolk sac tissue, respectively. Interestingly, the expression of other HES family members, such as Hes1 and Hes6 was not affected in these Notch models (data not shown), consistent with a context-dependent regulation of Notch targets [29, 30]; i.e. Notch signaling invokes distinct downstream targets depending on both the cell type and the local environment.
The expression of the endogenous Notch ligands and receptors was also examined in detail. Previous work has suggested a potential autoregulatory loop for Notch signaling in the control of ligands and receptors [31, 32]. Although expression of the Notch family receptors was not altered in either the EC-N1ICD or EC-Rbpj-KO yolk sacs, the Notch ligands Delta-like 4 (Dll4) and Jagged 1 (Jag1) showed higher expression in EC-N1ICD (2.5-fold and 2.0-fold, respectively) with little or no change in expression in EC-Rbpj-KO yolk sac. These findings point to specific Notch pathway targets in the extraembryonic yolk sac vasculature.
Notch-Rbpj signaling regulates vascular expression of key signaling molecules
Given the defects in vessel diameter and remodeling in response to altered Notch activity, we wished to focus gene expression analysis on secreted factors. Genes encoding several known and putative secreted factors were differentially expressed in the Notch models (Additional File 4). Expression defects of a subset of these genes were confirmed via RT-PCR, with a focus on genes with putative roles in vascular differentiation and that display coordinate defects in expression in the EC-N1ICD and EC-Rbpj-KO models. Although the expression of some VEGF family members was not significantly different in yolk sac, the microarray datasets indicated that expression of the VEGF family members Vegfc, encoding VEGF-C, and Pgf, encoding Placenta Growth Factor (PlGF), were both increased in EC-N1ICD and Vegfc was decreased in EC-Rbpj-KO (Additional File 4). RT-PCR confirmed that Vegfc was upregulated 2.6-fold in the EC-N1ICD and decreased to 8% in the EC-Rbpj-KO compared to control yolk sac tissue; Pgf was upregulated 1.9-fold in the EC-N1ICD and decreased to 74% in the EC-Rbpj-KO compared to control yolk sac tissue. The secreted cytokine Tgfb2 also exhibited increased expression in the yolk sacs of EC-N1ICD yolk sac tissue (4.7-fold) and decreased expression in EC-Rbpj-KO (49% of wild type levels) (Figure 4B).
The Notch-responsive genes identified in the previous analysis of whole yolk sac tissues were then examined in the sorted yolk sac endothelial cells. The endothelial marker, Pecam1 exhibited statistically equivalent expression between the EC-N1CD and wild type sorted cells, similar to that seen in whole yolk sac tissues (data not shown). However, expression of Hey1 and Heyl was increased 4.4-fold and 16.7-fold in the EC-N1ICD sorted endothelial cells respectively. Similarly, the VEGF family members Vegfc and Pgf were upregulated 2.1-fold and 1.9-fold, respectively in sorted EC-N1ICD endothelial cells compared to wild type, while Tgfb2 was increased 6.9-fold (Figure 5B). These results demonstrate that the expression of these genes is upregulated specifically in the endothelial lineages in EC-N1ICD yolk sac. Taken together, the gene expression defects demonstrate that Notch-Rbpj signaling acts to regulate many key genes specifically in the endothelia of the developing yolk sac. The misexpression of some of these factors may contribute to the vascular differentiation defects seen in the transgenic models.
Putative Notch regulated genes contain potential RBPJ binding sites in the upstream regulatory region
The in vivo studies have identified a number of putative Notch regulated genes in the developing vasculature. Many of these genes are known Notch targets, including Hey1 and Heyl, with known RBPJ binding sites within regulatory elements . These studies however also identified altered expression of several genes, including Vegfc, Tgfb2, and Pgf, for which their regulation by Notch signaling has not previously been described. Bioinformatic tools were used to determine if these genes are potentially direct targets of Notch signaling through RBPJ. Several RBPJ binding sites, with a core consensus binding sequence GTGGGAA , have been previously identified in known Notch targets such as the HES family members [28, 35]. The bioinformatic tool, ECR browser , was used to determine if canonical RBPJ binding sites were observed in the promoter proximal regions of loci encoding the secreted factors, Vegfc, Pgf, and Tgfb2. The mouse, human, and rat genomic sequences of each of the target genes were aligned and examined for the RBPJ conserved transcription factor binding sites (TFBS) within the evolutionary conserved regions (ECRs) upstream and downstream of the start site. In each of the genes a GTGGGAA consensus sequence was found, indicating a potential RBPJ site (Additional File 6). In the Vegfc locus, an RBPJ binding site is apparent approximately 7.8kb upstream of the start site, while the Pgf locus harbors an RBPJ binding site 4.1kb upstream and one 3.8kb downstream of the transcriptional start site. RBPJ sites were observed at approximately 2.5kb and 5.8kb upstream of the start site of the Tgfb2 locus. The putative Notch binding sites in each gene suggests that these genes may be direct targets of Notch signaling.
Numerous studies have indicated critical roles for Notch signaling in the proper formation and maintenance of the early vascular system in the mouse. However, the mechanisms by which Notch signaling regulates such diverse aspects of endothelial differentiation in its various contexts are active areas of research. Our analysis details the morphological and molecular defects associated with altered Notch signaling in vivo, with a focus on the extraembryonic vasculature of the yolk sac. This work has characterized distinct morphological defects in the early embryonic and extraembryonic vasculature associated with loss or gain of Notch activity. Substantial gene expression differences in these models point to putative Notch target genes, and suggest potential mechanisms by which Notch signaling directs endothelial cell differentiation.
Given that Notch signaling is associated with arterial identity [13, 14], this function of Notch signaling to control vessel size may represent an aspect of Notch function in the definition of the arteriole. The mechanisms of how Notch and other signaling pathways control vessel size in the various regions of the developing embryo are not well defined, and are likely context dependent. Some models suggest that directional cell division is important to dictate whether endothelial cell proliferation within a patent vessel results in an increase in luminal diameter . Other models suggest the recruitment of endothelial or angioblast cells into existing vessels as an important mechanism to increase vessel diameter . The remodeling process of the yolk sac makes extensive use of reallocation of cells during remodeling (discussed below), suggesting complex roles for Notch signaling in controlling endothelial cell behavior during remodeling.
The yolk sac vasculature represents a genetically tractable model to study endothelial differentiation, and the work presented here has made extensive analysis of this tissue. Endothelial differentiation within the yolk sac is initiated as groups of cells from the proximally situated extraembryonic mesoderm condense into blood islands by approximately embryonic day 7.0, which subsequently migrate toward the distal region of the yolk sac. The peripheral cells will differentiate to the endothelial cells lining the vasculature, whereas the inner cells become blood cells. The endothelial cells expand and fuse to form the vascular plexus, which is contiguous with the embryonic vasculature at the onset of blood flow . The capillaries are then remodeled into the hierarchical vascular network of vitelline artery, capillaries, and vitelline vein. Observations of vessel remodeling in the yolk sac, particularly in the chick embryo, suggest that the formation of large diameter vessels is from pre-existing capillary-derived endothelial cells. This process involves the collapse of some capillary microvessels, and the endothelial cells from the capillary are then recruited into the nascent vessels to result in a larger diameter vessel (Figure 2G, asterisk). This mode of remodeling resembles the process of intussusceptive arborization, which has been suggested to be an efficient and rapid mode of angiogenesis in a variety of sites . Although Notch signaling does play a significant role in sprouting angiogenesis  in many other contexts, the remodeling of the yolk sac in the early embryo may not utilize a sprouting angiogenesis method. Instead, this early vascular remodeling is a rather distinct vascular mechanism that is not entirely clear and remains to be studied in detail.
Based on this model of endothelial remodeling within the yolk sac plexus, the defects in vessel diameter and the remodeling failure in the Notch models used in this study indicate a possible defect in the reallocation of endothelial cells from capillaries into arterioles, suggesting that Notch plays a significant role in this migration. The phenotype observed in the EC-N1ICD yolk sac vasculature is possibly due to increased mobilization and disorganized recruitment of capillary-derived endothelial cells, resulting in a field of enlarged vessels. Conversely the failure of the yolk sac remodeling in the EC-Rbpj-KO model is due to the abrogation of endothelial cell migration to form the larger caliber vessels. Live imaging of the behavior of endothelial yolk sac cells in the Notch models used in this study will be required to elaborate this model.
A detailed understanding of the downstream effectors of Notch signaling during vascular differentiation in vivo, particularly within the extraembryonic tissues, has been lacking. The molecular analysis of the Notch models presented here identified a variety of gene expression defects associated with altered Notch activity. Both the gain- and loss-of-function in vivo models suggest misregulation of a number of genes. Data indicated that two of the Notch family ligands, Dll4 and Jag1 were upregulated in EC-N1ICD yolk sac, indicating a possible positive feedback loop for Notch signaling to direct expression of its ligands. There is some precedence for a positive regulatory loop in which Notch regulates the expression of ligands, including Jag1 in NIH 3T3 cells  and Dll1 in glioma cells .
Importantly, the gene expression analysis also identified a number of secreted factors whose expression within the endothelia is altered in the Notch models. These data suggest that the Notch signaling pathway regulates a number of secreted factors important in endothelial differentiation such as, Pgf, Vegfc, and Tgfb2, either directly or indirectly. Targeted deletions of select TGF-β signaling components in mice result in the improper formation of the yolk sac vasculature, indicating the importance for TGF-β signaling in the formation of the early vascular system . Vegf signaling has critical early roles in the formation of the blood vessels in the early embryo [43, 44]. VEGF has also been shown to act as an upstream component of Notch in the signaling cascade directing the differentiation of the zebrafish vasculature . PlGF has known roles in pathological angiogenesis [46, 47], and may act as a VEGF agonist by countering the effects of the VEGFA antagonist Flt1. In addition to the essential functions of VEGFC in the developmental origin of the lymphatic system [48, 49], there is conflicting data to suggest it plays a broader role in angiogenesis. Although this factor has known angiogenic activities in certain assays , no vascular defects have been reported in mice lacking Vegfc . The expression of VEGFC within the yolk sac however suggests a potential non-lymphatic function for this factor in this tissue. A putative role for VEGFC in angiogenesis may involve the modulation of its receptors, Kdr (VEGFR2) and Flt4 (VEGFR3), both of which play a critical role in early angiogenesis.
Notch signaling is essential for vessel remodeling, as our data shows that Notch regulates the expression of a variety of secreted factors. Notch signaling may play potential nonautonomous roles in the remodeling of the yolk sac capillary plexus (Figure 6B). Notch signaling is important for the regulation of select signaling molecules, including members of the VEGF family and TGF-β family, which would emanate from the developing arteriole where activity of Notch signaling is highest. These molecules then act in a paracrine and autocrine manner to elaborate the local arterial microenvironment about Notch expressing cells, which may potentially influence adjacent capillary endothelial cells, smooth muscle or mural cells, and hematopoietic cells. Indeed, nonautonomous functions for Notch signaling in the endothelium have been suggested, including attenuating proliferation of smooth muscle cells . Testing of the nonautonomous functions of Notch signaling in the vasculature will require determining the roles of Notch-regulated secreted factors in vivo. These experiments may include the use of conditional transgenics and knockouts of these factors in the endothelia, and determining remodeling and endothelial differentiation defects in these models.
Yolk sac vessel remodeling occurs after the initiation of blood flow, which initiates at approximately E8.5 in the mouse , and this flow is essential for the remodeling process. It is formally possible that the enlarged luminal diameter of the dorsal aorta in the Notch gain-of-function model could give rise to a secondary failure of yolk sac remodeling solely due to reduced flow and associated shear stress. However, the vessel architecture reported in mouse models with remodelling defects solely due to altered blood flow  display very different phenotypes from those observed in the N1ICD model. This comparison suggests that the remodeling defects and some of the associated gene expression defects in the yolk sac in this Notch1 gain-of-function model are not secondary to embryonic vessel defects or defects in blood flow. The cellular and subsequent molecular response to the shear stress associated with blood flow is not completely understood, but may involve a complex cell surface signalling molecules including KDR, PECAM1, and VE-cadherin . Shear stress thus initiates a molecular cascade in endothelial cells to direct further morphological changes to direct remodeling. An important question is what is the link between blood flow and Notch signaling, both of which are essential for vessel remodeling. During remodeling, an arteriole rudiment is first observed at the site of yolk sac vasculature contact with the omphalomesentric artery. Early Dll4 expression is observed at this arteriole rudiment within the yolk sac , indicating it is likely the Notch ligand that initiates Notch activity within this restricted region of the yolk sac vasculature. It remains to be seen the extent to which fluid dynamics control the expression of Notch signalling components and the subsequent remodeling process.
The transcriptional network controlled by Notch signaling during extraembryonic endothelial differentiation is largely unknown. The present data demonstrate a role for Notch signaling in the regulation of a number of key genes in the embryonic vasculature of the yolk sac, including a variety of secreted factors important for endothelial differentiation. These downstream targets suggest a mechanism for Notch regulation of vessel diameter size during the remodeling process in the yolk sac vasculature. Further work on the in vivo models will help to further define the relationship between the transcriptional networks regulated by Notch to direct endothelial differentiation, and the role of these Notch downstream targets in endothelial migration and vascular remodeling. The understanding of these interactions and processes will aid in the development of treatments affecting vascular differentiation, including heart disease and tumor progression.
Mice and embryos
The generation of Rosa Notch mice, loxP-flanked Rbpj, Tie2-Cre and Flk1-Cre mice have been described previously [21, 22, 23, 24]. Tie2-Cre mice and loxP-flanked Rbpj mice were mated to obtain Tie2-Cre; Rbpj f/+ males. Breeding pairs of Tie2-Cre or Flk1-Cre mice and Rosa Notch mice or Tie2-Cre; Rbpj f/+ males and Rbpj f/+ females were intercrossed and the presence of a vaginal plug was identified as E0.5. Embryos were dissected from the decidual tissue at E8.5, E9.5, or E10.5 and treated according to the intended protocol. Placenta were separated from the embryo along with the mesometrial portion of the decidua and prepared for histology. All experimental procedures were performed with prior approval of the University Institutional Animal Care and Use Committee in accordance with guidelines established by the American Veterinary Medical Association.
Genotyping of Progeny
The genotypes of all offspring were analyzed by PCR on genomic DNA isolated from ear punches, yolk sac samples, or embryonic tissue depending on the intended protocol. Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen). The Tie2-Cre and Flk1-Cre transgene presence was tested with the primers Cre-R3, 5'-AAT GCT TCT GTC CGT TTG-3' and Cre-F3, 5'-GGA TTA ACA TTC TCC CAC C-3', giving a 458-bp band . PCR genotyping for Rosa Notch mice was performed as described . The wild type, floxed, and deleted Rbpj alleles were genotyped as described .
Embryos with or without the surrounding yolk sac were fixed in 4% paraformaldehyde/PBS overnight at 4°C. The following day they were rinsed in PBS, dehydrated in methanol, and bleached in 1mL of 1.5% H2O2 in methanol for 4-5 hours at room temperature. Embryos were then rehydrated through methanol into PBS, blocked in PBSMT (PBS, 3% nonfat dry milk, 0.1% Triton X-100) for 2 hours at room temperature and incubated with 1:50 anti-PECAM1 antibody (BD Pharmingen) overnight at 4°C. Embryos were washed 5 times for 1 hour each with PBSMT and incubated with 1:100 HRP-coupled anti-rat IgG (#14-16-12, kpl.com) overnight at 4°C. Embryos were again washed 5 times in PBSMT with a final wash in PBT (PBS, 0.2% BSA, 0.1% Triton X-100). Then embryos were incubated in developing solution (0.3 mg/mL DAB, 0.5% NiCl2 in PBT) for 20 minutes at room temperature followed by the addition of H2O2 at a 0.03% final concentration. Once the color developed (approximately 10min.), the embryos were rinsed in PBT and then PBS and fixed overnight in 2% paraformaldehyde/0.1% gluteraldehyde/PBS at 4°C. Embryos were then rinsed in PBS and either equilibrated into 70% glycerol for imaging or dehydrated in ethanol for paraffin embedding.
Untreated whole-mount E8.0 and E8.5 embryos with surrounding yolk sac were fixed on ice for 10 min in a fixation solution. The samples were then washed 3 times in 1X PBS pH 7.4 and stained overnight at 37°C per established procedures . The next day, the samples were rinsed in PBS and fixed overnight in a 3.7% paraformaldehyde/PBS solution. The following day, the samples were again rinsed, imaged and stored in 70% EtOH for paraffin embedding.
Untreated whole-mount embryos, whole-mount PECAM1 stained embryos and placenta were fixed in 3% paraformaldehyde overnight, dehydrated into 70% ethanol and embedded in paraffin wax. Embryos were then sectioned on the transverse generally in 8 μm sections. For histological observation, Hematoxylin and Eosin or Nuclear Fast Red staining was conducted on the paraffin sections and sections were observed.
Preparation of single yolk sac cell suspension
E9.5 embryos were obtained from timed matings between Flk1-Cre females and Rosa Notch males. Yolk sacs were incubated in 0.1% collagenase (StemCell Technologies Inc)/phosphate-buffered saline (PBS)/20% fetal bovine serum at 37°C for 30 minutes. The digested yolk sacs were aspirated through 27-gauge needles to fully separate the cells. Genotyping was performed on DNA isolated from corresponding embryo tissues.
Cell sorting with PECAM1 and analysis of PECAM1+ cells
Single-cell suspensions from yolk sacs were incubated for 30 minutes at 4°C with anti-PECAM1 antibody conjugated to phycoerythrin cyanine 7 (eBioscience Inc). The PECAM1+ cells were isolated by cell sorting with the use of a BD FACSAria cell sorter (BD Biosciences). Total RNA was isolated from the sorted cells using an RNeasy mini kit (Qiagen) and RNA was used for real time PCR analysis.
Embryos were dissected at E9.5. The uterus and decidua were carefully removed and discarded. The yolk sac was separated from the embryo and the embryo was used for genotyping. Total RNA was isolated from the yolk sac using an RNeasy mini kit (Qiagen). cDNA was generated using SuperScript III reverse transcriptase (Invitrogen). Semiquantitative RT-PCR was performed using a number of primers from IDT (idtdna.com) (Additional File 7), with ribosomal protein L7 (5'-GAA GCT CAT CTA TGA GAA GGC-3' and 5'-AAG ACG AAG GAG CTG CAG AAC-3') as a control. The annealing temperature and number of PCR cycles was optimized for each reaction.
Real Time PCR
RNA was isolated from sorted endothelial cells using an RNeasy mini kit (Qiagen). cDNA from endothelial cells was generated using Superscrpit III reverse transcriptase (Invitrogen) and quantitative real-time PCR analysis was performed using Taqman primer sets with the 7500 Real Time PCR system (Applied Biosystems). Gene expression was normalized to GAPDH. Specific ABI Taqman primer/probe assay IDs are available upon request.
RNA was isolated from yolk sac tissues using an RNeasy mini kit (Qiagen). RNA was initially analyzed with the Mouse Genome 430 A Array from Affymetrix. Microarray data was deposited to the Gene Expression Omnibus (GSE22418).
Yolk sac immunofluorescence
Embryos were dissected at E9.5 in PBS. The uterus and decidua were carefully removed and discarded. The yolk sac was separated from the embryo by severing the vitelline arteries and the embryo was used for genotyping. The yolk sacs were collected in separate wells of a 24 well plate and then fixed in 1mL of 3% paraformaldehyde/PBS for 15 minutes on ice. Then the yolk sacs were permeabilized in 1mL of PBS containing 0.02% Triton X-100 for 30 minutes. Yolk sacs were transferred to separate 1.5mL Epindorf tubes containing 500uL of blocking solution (PBS, 3% BSA, 5% donkey serum (Jackson ImmunoResearch)) for 2 hours at room temperature. Blocking solution was changed once at 1 hour. Yolk sacs were then incubated with 1:33 anti-PECAM1 antibody (#557355, BD Pharmingen) overnight at 4°C. Embryos were washed 5 times for 1 hour each with blocking solution and incubated with 1:75 FITC-conjugated AffiniPure Anti-Rat IgG (#712-095-153, Jackson ImmunoResearch) overnight at 4°C. Embryos were again washed 5 times in blocking solution with a final 20-minute wash in PBS. The yolk sacs were transferred via Pasteur pipette to a drop of SlowFade Gold antifade reagent (Molecular Probes) on a glass slide and covered with a cover slip.
Embryos were prepared following the above protocol for histology. Tissues were deparrafinized through xylene and EtOH into cold tap water. Antigen retrieval was performed by immersing the slides in 10 mM calcium citrate for 20 minutes in a steam chamber. Sections were washed first with water and then 2 times with PBS. Sections were then incubated in PBT (PBS, 0.2% BSA, 0.1% Triton X-100) for 2 hours at room temperature. Sections were stained with a-smooth muscle actin (1:250 dilution; catalog no. A2547; Sigma-Aldrich, Inc) for 1 hour at 4°C. Sections were washed 3 times with PBT and then stained with Alexa Fluor 546 goat anti-mouse IgG (1:250 dilution; catalog no. A11003; Invitrogen) for 30 minutes at room temperature in the dark. Sections were again washed 3 times with blocking solution. A small amount of ProLong Gold antifade reagent with DAPI (Invitrogen) was applied and the samples were covered with a cover slip, allowed to set, and imaged. Slides were stored at -20°C.
Identification of TFBSs
The ECR browser  was used to determine the location of potential RBPJ binding sites. The evolutionary conserved regions of the mouse, human, and rat were examined upstream and flanking the transcription start site of each gene for the presence of the RBPJ binding sequence (GTGGGAA) .
Microscopy and image acquisition
Images were acquired with a Nikon SMZ800 dissecting microscope for whole embryos and a Nikon ECLIPSE 55i for embryonic sections using a Leica DFC480 camera and Leica FireCam 3.0 software. Images for yolk sac immunofluorescence were acquired with an Olympus 1X71 microscope and Olympus DP71 camera using Olympus DP71 controller software. Adobe Photoshop CS2 was used for photograph editing.
Data bars represent the means +/- standard error of the mean. RNA analyses of yolk sac tissues were performed with an n of 5. The statistical significance of the data was determined using a t-test with a p-value of < 0.05 considered statistically significant.
We thank Stanton Fernald of the KU Medical Center Imaging Core for aid in image acquisition, Lucy Xiu for technical assistance, Katie Burgess for assistance with statistical analysis, the KUMC-Microarray Facility for generating array data sets, and the KUMC Flow Cytometry Core. This work was supported by an American Heart Association grant 0665481Z and National Institutes of Health COBRE grant P20RR024214 (to JLV) and an American Heart Association Heartland Affiliate predoctoral fellowship 0715516Z (to JNC).
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