Abstract.
In view of recent theories of “active” membranes, we have studied multilamellar phospholipid membrane stacks with reconstituted transmembrane protein bacteriorhodopsin (BR) under different illumination conditions by X-ray scattering. The light-active protein is considered as an active constituent which drives the system out of equilibrium and is predicted to change the collective fluctuation properties of the membranes. Using X-ray reflectivity, X-ray non-specular (diffuse) scattering, and grazing incidence scattering, we find no detectable change in the scattering curves when changing the illumination condition. In particular the intermembrane spacing d remains constant, after eliminating hydration-related artifacts by design of a suitable sample environment. The absence of any observable non-equilibrium effects in the experimental window is discussed in view of the relevant parameters and recent theories.
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Giahi, A., El Alaoui Faris, M., Bassereau, P. et al. Active membranes studied by X-ray scattering. Eur. Phys. J. E 23, 431–437 (2007). https://doi.org/10.1140/epje/i2007-10203-7
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DOI: https://doi.org/10.1140/epje/i2007-10203-7