Russian Journal of Physical Chemistry B

, Volume 4, Issue 8, pp 1211–1216 | Cite as

Subcritical water extraction of biologically active substances from milk thistle seed (Silybum murianum L.)

  • I. A. Platonov
  • N. V. Nikitchenko
  • L. A. Onuchak
  • Yu. I. Arutyunov
  • V. A. Kurkin
  • P. V. Smirnov


A method of subcritical water extraction (SCWE) of biologically active substances from milk thistle seed (Silybum marianum L.) that makes it possible to obtain aqueous extracts without using organic solvents is suggested. The contents of hepatoprotective biologically active substances (taxifolin, silychristin, silydianin, and silybin) in milk thistle seed extracts obtained by SCWE and by standardized liquid extraction procedures are compared.


extraction subcritical water extraction biological active substances milk thistle taxifolin silychristin silydianin silybin flavolignans high-performance liquid chromatography 


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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • I. A. Platonov
    • 1
  • N. V. Nikitchenko
    • 1
  • L. A. Onuchak
    • 1
  • Yu. I. Arutyunov
    • 1
  • V. A. Kurkin
    • 2
  • P. V. Smirnov
    • 1
  1. 1.Samara State UniversitySamaraRussia
  2. 2.Samara State Medical UniversitySamaraRussia

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